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Team:Evry/Interlab Study/08-29-2014
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<br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2> | <br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2> | ||
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| - | + | Selected colonies PCR products from the 28/08/2014 were purified with the NucleoSpin kit (Macherey Nagel). DNA was quantify by Nanodrop 2000. | |
| - | + | <ul> | |
| + | <li> Colony n°2: 41.4 ng/µl | ||
| + | <li> Colony n°3: 65.8 ng/µl | ||
| + | <li> Colony n°4: 27.4 ng/µL | ||
| + | </ul> | ||
| + | <br/> Colony n°3 was sent to sequence with the identification 26AE07 (VF2) and 26AE08 (VR) | ||
| + | <br/> | ||
| + | <br/> | ||
| + | <h2>Other constructions of the Anderson library of constitutive promoters</h2> | ||
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Transformation in DH5 alpha chemocompetent <i>E. coli</i> as doing on 21th August, with ampicilline LB agar plates | Transformation in DH5 alpha chemocompetent <i>E. coli</i> as doing on 21th August, with ampicilline LB agar plates | ||
<br/> Incubation overnight at 37°C. | <br/> Incubation overnight at 37°C. | ||
| + | <br/> | ||
<br/> | <br/> | ||
<span class="cd-date">Aug 29</span> | <span class="cd-date">Aug 29</span> | ||
Latest revision as of 02:02, 20 September 2014
Construction n°1: PSB1C3 with I20260
Selected colonies PCR products from the 28/08/2014 were purified with the NucleoSpin kit (Macherey Nagel). DNA was quantify by Nanodrop 2000.
- Colony n°2: 30.7 ng/µl and A260/280 1.92
- Colony n°4: 52.3 ng/µl and A260/280 1.75
- Colony n°7: 26.1 ng/µL and A260/280 1.72
Samples were send to sequencing. N° 26DJ96, 26DJ97, 26DJ98, 26DJ99, 26EA00 and 26EA01.
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Selected colonies PCR products from the 28/08/2014 were purified with the NucleoSpin kit (Macherey Nagel). DNA was quantify by Nanodrop 2000.
- Colony n°2: 41.4 ng/µl
- Colony n°3: 65.8 ng/µl
- Colony n°4: 27.4 ng/µL
Colony n°3 was sent to sequence with the identification 26AE07 (VF2) and 26AE08 (VR)
Other constructions of the Anderson library of constitutive promoters
Transformation in DH5 alpha chemocompetent E. coli as doing on 21th August, with ampicilline LB agar plates
Incubation overnight at 37°C.
Aug 29
