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Team:UNIK Copenhagen/Quenchibodies
From 2014.igem.org
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<p>Gene construct 2: ProXtag-HeavyChain-HIStag</p> | <p>Gene construct 2: ProXtag-HeavyChain-HIStag</p> | ||
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<area shape="rect" coords="58,2,169,50" title="Viable part of the heavy chain" type="button" onclick="heavyVFunction();"> | <area shape="rect" coords="58,2,169,50" title="Viable part of the heavy chain" type="button" onclick="heavyVFunction();"> | ||
<area shape="rect" coords="170,2,280,50" title="Conserved part of the heavy chain" type="button" onclick="heavyCFunction();"> | <area shape="rect" coords="170,2,280,50" title="Conserved part of the heavy chain" type="button" onclick="heavyCFunction();"> | ||
| - | <area shape="rect" coords="281,2,336,50" title=" | + | <area shape="rect" coords="281,2,336,50" title="His-tag" type="button" onclick="histFunction();"> |
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<table class="sequence_description" id="about_gene"></table></div> | <table class="sequence_description" id="about_gene"></table></div> | ||
Latest revision as of 18:55, 11 September 2014
QUENCHIBODIES
The Ultra Quenchibody Project focuses on the construction of a FAB covalently linked to a fluorophore at the N-terminal site. Light emission from the attached fluorophore is quenched by tryptophan residues in the FAB fragment.
Upon binding of the antigen, the FAB fragment undergoes structural changes, which displace the fluorophore away from the tryptophan residues eliminating the quenching effect. In this way binding of the antigen results in fluorescence.
Estradiol and progesterone are both environmentally damaging hormones, which in excess are environmentally hazardous due to interference with the reproductive systems of animals, and can lead to various types of cancer including breast and uterus cancer. Within the Quenchibody project we will construct two fluorescent detection FAB fragments. The FAB fragments will be derived from antibodies binding to the sex hormones estradiol and progesterone. These products will thus allow for fast and easy step detection method of the levels of both hormones.
GENE CONSTRUCTS
Touch the lego bricks to see what sequences the gene consist of and click on the sequences to read more about their function. Note that the information box will be shown under the pictures.
Gene construct 1: LightChain-FLAG
Gene construct 2: ProXtag-HeavyChain-HIStag
