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Team:Evry/Notebook/Transformation/09-02-2014
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| + | <u> <b> <big><FONT COLOR=#003333> Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Results </font></big></b> </u> <br> | ||
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| + | <li> Positive control with E.Coli : <br> | ||
| + | <div align="center"><img src="https://static.igem.org/mediawiki/2014/0/05/ColiTransfo.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" style="width:60%;height:60%" /> <br> | ||
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<li> Resulst with Pseudovibrio : <br> | <li> Resulst with Pseudovibrio : <br> | ||
| - | <img src="https://static.igem.org/mediawiki/2014/f/f5/PlatesPseudoTransfo.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" /> <br> | + | <div align="center"><img src="https://static.igem.org/mediawiki/2014/f/f5/PlatesPseudoTransfo.jpg" alt="GROSSE NAZE T'AS PAS REUSSI A METTRE L'IMAGE" style="width:60%;heigth:60%" /> </div><br> |
NB: The negative control with E.Coli does'nt work on any plates. The culture was probably too old. <br> | NB: The negative control with E.Coli does'nt work on any plates. The culture was probably too old. <br> | ||
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A problem occurs with stocks of electro-competent Pseudovibrio because wa can see that it growth on Kanamycine 1:1000. | A problem occurs with stocks of electro-competent Pseudovibrio because wa can see that it growth on Kanamycine 1:1000. | ||
On Chloramphenicol, the contamination does'nt seem to grow. <br> | On Chloramphenicol, the contamination does'nt seem to grow. <br> | ||
Latest revision as of 21:58, 17 October 2014
Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Results
NB: The negative control with E.Coli does'nt work on any plates. The culture was probably too old.
A problem occurs with stocks of electro-competent Pseudovibrio because wa can see that it growth on Kanamycine 1:1000. On Chloramphenicol, the contamination does'nt seem to grow.
We have to prove that it's Pseudovibrio wich growth on Chloramphenicol. Sep 02
