Team:Brasil-SP/Notebook

From 2014.igem.org

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<h1 >WELCOME TO iGEM 2014! </h1>
 
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<p>Your team has been approved and you are ready to start the iGEM season!
 
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
 
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<br>
 
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Brasil-SP/Notebook&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
 
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<h1 align=center>Main Assembly Map</h1>
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<a href="https://2014.igem.org/Team:Brasil-SP"style="color:#000000">Home </a> </td>
 
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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<p><div align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The main assembly map describes all the constructions that would be done in the project, but some of them have not been performed in time of the iGEM deadline. The succesfully constructed assemblies is indicated by a green check point and the unsuccessfull constructions are indicated by a red "X". Click on these assemblies to access the lab form.</div></p>
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<a href="https://2014.igem.org/Team:Brasil-SP/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Brasil-SP"style="color:#000000"> Official Team Profile </a></td>
 
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<div align = center>
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<a href="https://2014.igem.org/Team:Brasil-SP/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:Brasil-SP/Parts"style="color:#000000"> Parts</a></td>
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  <div class="map_image" style="background-image: url('https://static.igem.org/mediawiki/2014/8/87/Mapa.png');">
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<a href="https://2014.igem.org/Team:Brasil-SP/Modeling"style="color:#000000"> Modeling</a></td>
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<a class="map_link" id="map_link_1" title="" href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>
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<a class="map_link" id="map_link_2" title="" href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>
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<a class="map_link" id="map_link_3" title="" href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a>
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<a class="map_link" id="map_link_4" title="" href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>
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<a class="map_link" id="map_link_5" title="" href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>
 +
<a class="map_link" id="map_link_6" title="" href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a>
 +
<a class="map_link" id="map_link_7" title="" href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>
 +
<a class="map_link" id="map_link_8" title="" href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>
 +
<a class="map_link" id="map_link_9" title="" href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>
 +
<a class="map_link" id="map_link_10" title="" href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>
 +
        <a class="map_link" id="map_link_11" title="" href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a>
 +
  </div>
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<h1 align=center>Assemblies forms</h1>
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<a href="https://2014.igem.org/Team:Brasil-SP/Notebook"style="color:#000000"> Notebook</a></td>
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<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
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<p><strong>Assembly form template</strong></p>
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<a href="https://2014.igem.org/Team:Brasil-SP/Safety"style=" color:#000000"> Safety </a></td>
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<p>This is the template designed to help with the laboratory organization during the assemble of biological parts. If you want to use the assembly method we used you can print the form and complete it for each construction. Hope it helps :)</p>
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<div align=center>
-
<a href="https://2014.igem.org/Team:Brasil-SP/Attributions"style="color:#000000"> Attributions </a></td>
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<a href="https://static.igem.org/mediawiki/2014/e/eb/Assembly_Form.pdf"><img src="https://static.igem.org/mediawiki/2014/3/32/Assembly_form_pg3.png" style="width:700px;height:400px"/></a>
 +
</div>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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-
</tr>
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 +
<h1 align=center><strong>Life Inside the LAB</strong></h1>
 +
 
 +
<thead><h2 align=center>July</h2>
 +
 
 +
</thead>
 +
 
 +
 
 +
<tbody>
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 +
<table>
 +
  <tr>
 +
    <th style="background-color:#bfacc0">Monday</th>
 +
    <th style="background-color:#bfacc0">Tuesday</th>
 +
    <th style="background-color:#bfacc0">Wednesday</th>
 +
    <th style="background-color:#bfacc0">Thursday</th>
 +
    <th style="background-color:#bfacc0">Friday</th>
 +
    <th style="background-color:#bfacc0">Saturday</th>
 +
    <th style="background-color:#bfacc0">Sunday</th>
 +
  </tr> <tr>
 +
    <td>30/06
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> lasR (<a href="http://parts.igem.org/Part:BBa_C0079" style="color:#3ab473">BBa_C0079</a>). The purpose of this PCR was to add the RBS (<a href="http://parts.igem.org/Part:BBa_K143021" style="color:#3ab473">BBa_K143021</a>), through addition of the sequence in the primer foward, and also to remove the LVA tag. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this part will be called <a href="http://parts.igem.org/Part:BBa_K1521001" style="color:#3ab473">BBa_K1521001</a>.</li>
 +
    </ul>
 +
    </td>
 +
    <td>01/07
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> of lasR <a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> product </li>
 +
    </ul>
 +
    </td>
 +
    <td>02/07
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the Biobricks:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li>
 +
      </ul>
 +
      <li>Inoculum of the Biobricks sent by iGEM HQ:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316018" style="color:#3ab473">BBa_K316018<a/></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316015" style="color:#3ab473">BBa_K316015<a/></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>03/07
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the Biobricks sent by the Imperial College Team:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016<a/></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K143031" style="color:#3ab473">BBa_K143031<a/></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification of the plasmidial DNA samples and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316018" style="color:#3ab473">BBa_K316018<a/></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316015" style="color:#3ab473">BBa_K316015<a/></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>04/07</td>
 +
    <td>05/07</td>
 +
    <td>06/07
 +
    <ul>
 +
      <li>Inoculum:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
  </tr>
 +
<tr>
 +
    <td>07/07
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li>
 +
        <li<a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
 +
      <ul>
 +
        <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>08/07</td>
 +
    <td>09/07
 +
    <ul>
 +
    <li>Inoculum:</li>
 +
      <ul>
 +
        <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>10/07
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li>
 +
      <ul>
 +
        <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a> (restriction analysis fail, repeat)</li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> the qteE gene for amplification. We also added the RBS (<a href="http://parts.igem.org/Part:BBa_K143021" style="color:#3ab473">BBa_K143021,</a>) using the foward primer. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this biobrick will be called <a href="http://parts.igem.org/Part:BBa_K1521000" style="color:#3ab473">BBa_K1521000</a>.</li>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of RBS+lasR in PTZ57R/T vector (instaclone kit)</li>
 +
    </ul>
 +
    </td>
 +
    <td>11/07
 +
    <ul>
 +
      <li>Prepare 40% glycerol solution</li>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
 +
      <ul>
 +
        <li>RBS + lasR (<a href="http://parts.igem.org/Part:BBa_C0079"style="color:#3ab473">BBa_C0079</a>)</li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a>:</li>
 +
      <ul>
 +
        <li>RBS + qteE</li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>12/07</td>
 +
    <td>13/07</td>
 +
  </tr> <tr>
 +
    <td>14/07
 +
    <ul>
 +
      <li>Assembly:<a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
      <li>Prepare X-gal</li>
 +
      <li>Prepare more LB medium (solid + liquid)</li>
 +
    </ul>
 +
    </td>
 +
    <td>15/07
 +
    <ul>
 +
      <li>Assembly:<a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a></li>
 +
      <ul>
 +
        <li>Quantification of digestion EXSP products</li>
 +
      </ul>
 +
      <li>Replate RBS+lasR</li>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of <a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> qteE in pGEM T-easy vector</li>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> <a href="" style="color:#3ab473"> BBa_J04450</a></a></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li>
 +
      <ul>
 +
        <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>16/07
 +
    <ul>
 +
    <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151">Transformation</a>:</li>
 +
      <ul>
 +
      <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></li>
 +
      <li>RBS+qteE</li>
 +
      </ul>
 +
    <li>Electrophoresis analysis:</li>
 +
      <ul>
 +
      <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>17/07
 +
    <ul>
 +
      <li>Glycerol Stock and <a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:<li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li>
 +
        <li>RBS+lasR</li>
 +
        <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (EP)</li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (XS)</li>
 +
      </ul>
 +
      <li>Inoculum:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>18/07
 +
    <ul>
 +
      <li>Glycerol Stock and <a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li>
 +
        <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (EP)</li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (XS)</li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>19/07</td>
 +
    <td>20/07</td>
 +
  </tr> <tr>
 +
    <td>21/07
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li>
 +
      </ul>
 +
      <li>Inoculum:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
 +
        <li>RBS+qteE</li>
 +
      </ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>22/07
 +
    <ul>
 +
      <li>Inoculum:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li>
 +
      <ul>
 +
        <li>RBS+qteE</li>
 +
      </ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>23/07
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      </ul>
 +
      <li>Prepare more <a href="https://static.igem.org/mediawiki/2014/9/96/Competent_Cells_using_Calcium_Chloride.pdf" style="color:#f05151"> competent cells</a></li>
 +
      <li>Prepare LB medium (solid and liquid)</li>
 +
      <li>Inoculum</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li>
 +
      </ul>
 +
      <li>Prepare RBS+lasR and RBS+qteE for sequencing</li>
 +
    </ul>
 +
    </td>
 +
    <td>24/07
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>25/07
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
 +
      <ul>
 +
        <li>Glycerol Stock</li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>26/07</td>
 +
    <td>27/07</td>
 +
  </tr> <tr>
 +
    <td>28/07
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (+NdeI enzyme)</li>
 +
        <li>OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that both the construction and the pSB1C3 vector had similar size.</li>
 +
      </ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>29/07
 +
    <ul>
 +
      <li>>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>30/07
 +
    <ul>
 +
      <li>>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      </ul>
 +
    </ul>   
 +
    </td>
 +
    <td>31/07
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> for RBS removal</li>
 +
      <li>OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this  Lac promotor :) </li>
 +
      <li>PCR of Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> was followed by a <a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> and <a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> in the PUC19 vector</li>
 +
    </ul>
 +
    </td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
  </tr>
</table>
</table>
 +
<br>
 +
<br>
 +
<br>
-
</tr>
 
 +
</tbody>
-
</tr>
+
<thead><h2 align=center>August</h2>
-
+
 +
</thead>
 +
</tbody>
 +
<table style="width:100%">
 +
  <tr>
 +
    <th style="background-color:#bfacc0">Monday</th>
 +
    <th style="background-color:#bfacc0">Tuesday</th>
 +
    <th style="background-color:#bfacc0">Wednesday</th>
 +
    <th style="background-color:#bfacc0">Thursday</th>
 +
    <th style="background-color:#bfacc0">Friday</th>
 +
    <th style="background-color:#bfacc0">Saturday</th>
 +
    <th style="background-color:#bfacc0">Sunday</th>
 +
  </tr> <tr>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
    <td>01/08
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
 +
      </ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
 +
      <ul>
 +
        <li>Glycerol Stock</li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>02/08</td>
 +
    <td>03/08</td>
 +
  </tr>
 +
<tr>
 +
    <td>04/08
 +
    <ul>
 +
      <li>Inoculum</li>
 +
      <ul>
 +
        <li>E. coli DH5-alpha</li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a></li>
 +
        <li>Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li>
 +
      </ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li>
 +
      <ul>
 +
        <li>Prepare more inoculum</li>
 +
        <li>Repeat <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>05//08
 +
    <ul>
 +
      <li>Prepare assemblies <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a> for cytometry characterization</li>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li>
 +
      <ul>
 +
        <li>Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
 +
      </ul>
 +
      <li>Inoculum</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li>
 +
      </ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
 +
        <li>And once again! <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (this <a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a> is tough)</li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>06/08</td>
 +
    <td>07/08
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>08/08</td>
 +
    <td>09/08</td>
 +
    <td>10/08</td>
 +
  </tr> <tr>
 +
    <td>11/08
 +
    <ul>
 +
      <li>Prepare Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> for sequencing</li>
 +
      <li>Analyse the RBS+qteE and RBS+lasR sequencing file</li>
 +
    </ul>
 +
    </td>
 +
    <td>12/08
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li>
 +
      <ul>
 +
        <li>RBS+qteE</li>
 +
        <li>RBS+lasR</li>
 +
        <li>OBS: this digestion was performed so we clone this parts in the pSB1C3 vector to put them in Biobrick standard</li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>13/08
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>14/08
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> and <a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a> in pSB1C3 for BBiobrick standard:</li>
 +
      <ul>
 +
        <li>RBS+qteE</li>
 +
        <li>RBS+lasR</li>
 +
      </ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
        <li>OBS: we had some problems here with the purification and we lost all our digestions, so we're repeating the digestion</li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>15/08</td>
 +
    <td>16/08
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>17/08
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
  </tr> <tr>
 +
    <td>18/08
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
      </ul>
 +
      <li>Prepare LB medium (liquid and solid)</li>
 +
    </ul>
 +
    </td>
 +
    <td>19/08
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>20/08
 +
    <ul>
 +
      <li>RBS+qteE, RBS+lasR and Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>21/08
 +
    <ul>
 +
      <li>RBS+qteE, RBS+lasR and Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
 +
      <ul>
 +
        <li>Glycerol Stock</li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf">Miniprep</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>22/08
 +
    <ul>
 +
      <li>Prepare LB medium (liquid and solid)</li>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a>:</li>
 +
      <ul>
 +
        <li>Repeat <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">restriction analysis</a> adding the enzyme NdeI.</li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>23/08</td>
 +
    <td>24/08</td>
 +
  </tr> <tr>
 +
    <td>25/08
 +
    <ul>
 +
      <li>Inoculum</li>
 +
      <ul>
 +
        <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li>
 +
        <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> with higher melting temperature.</li>
 +
    <ul>
 +
    </td>
 +
    <td>26/08
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
 +
        <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>27/08
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
 +
        <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>28/08</td>
 +
    <td>29/08</td>
 +
    <td>30/08</td>
 +
    <td>31/08</td>
 +
  </tr>
 +
</table>
 +
<br>
 +
<br>
 +
<br>
-
</td>
+
<thead><h2 align=center>September</h2>
-
<tr> <td colspan="3"  height="15px"> </td></tr>
+
</thead>
-
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
+
-
<tr> <td colspan="3"  height="5px"> </td></tr>
+
 +
<tbody>
 +
 +
<table style="width:100%">
 +
  <tr>
 +
    <th style="background-color:#bfacc0">Monday</th>
 +
    <th style="background-color:#bfacc0">Tuesday</th>
 +
    <th style="background-color:#bfacc0">Wednesday</th>
 +
    <th style="background-color:#bfacc0">Thursday</th>
 +
    <th style="background-color:#bfacc0">Friday</th>
 +
    <th style="background-color:#bfacc0">Saturday</th>
 +
    <th style="background-color:#bfacc0">Sunday</th>
 +
  </tr> <tr>
 +
    <td>01/09
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a> of the part BII with EcoRV.
 +
      </ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
 +
        <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>02/09
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
 +
        <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>03/09
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
 +
        <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>04/09
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
 +
        <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
 +
        <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
 +
      <ul>
 +
        <li>Glycerol Stock</li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>05/09</td>
 +
    <td>06/09</td>
 +
    <td>07/09</td>
 +
  </tr>
 +
<tr>
 +
    <td>08/09
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf" style="color:#f05151">QuickChange PCR</a> to mutate the cleavage site of the <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037</a> to a Cathepsin S recognition site</li>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li>
 +
    </ul>
 +
    </td>
 +
    <td>09/09</td>
 +
    <td>10/09
 +
    <ul>
 +
      <li>The QuickChange protocol didn't work well. So try again with more attention!</li>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf" style="color:#f05151">QuickChange PCR</a> to mutate the cleavage site of the <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037</a> to a Cathepsin S recognition site</li>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>11/09
 +
    <ul>
 +
      <li>Prepare inoculum of the mutated <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
      </ul> 
 +
    </ul>
 +
    </td>
 +
    <td>12/09
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151">Transformation</a></li>
 +
      </ul>
 +
      <li>We had far to many difficulties trying to remove the RBS from the <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a>, so we decided to synthesize it using PCR.</li>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/3/35/PCR_for_synthesis.pdf" style="color:#f05151">PCR for synthesis</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a> (<a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> without RBS)</li>
 +
    </ul>
 +
    </td>
 +
    <td>13/09
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
 +
      <ul>
 +
        <li>Repeat <a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
      </ul>
 +
      <li>Prepare LB medium (solid and liquid)</li>
 +
    </ul>
 +
    </td>
 +
    <td>14/09
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a>
 +
      <ul>
 +
        <li>Glycerol Stock</li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
 +
      </ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
  </tr> <tr>
 +
    <td>15/09
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li>
 +
    </ul>     
 +
    </td>
 +
    <td>16/09
 +
    <ul>
 +
      <li>Prepare 40% glycerol solution</li>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
 +
      <ul>
 +
        <li>Glycerol Stock</li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>17/09
 +
    <ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of the synthesized <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a> in TOPO vector followed by <a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
    </ul>
 +
 +
    <ul>
 +
      <li>Measurement Interlab Study - Samples growth biobrick devices 1, 2 and 3</li>
 +
    </ul>
 +
   
 +
    </td>
 +
    <td>18/09
 +
    <ul>
 +
      <li>Replate <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a>. We forgot the X-gal :(</li>
 +
    </ul>
 +
    <ul>
 +
      <li>Measurement Interlab Study - Sample preparation and characterization by Flow Cytometry and Fluorometry</li>
 +
    </ul>
 +
    </td>
 +
    <td>19/09</td>
 +
    <td>20/09</td>
 +
    <td>21/09</td>
 +
  </tr> <tr>
 +
    <td>22/09
 +
    <ul>
 +
      <li>Inoculum of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
   
 +
    </td>
 +
    <td>23/09
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
      <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf">Miniprep</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li>
 +
    </ul>
 +
    </td>
 +
    <td>24/09
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>25/09
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>26/09
 +
    <ul>
 +
      <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
 +
      <ul>
 +
        <li>Glycerol Stock</li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
      <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
 +
                    <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
 +
                    <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>27/09</td>
 +
    <td>28/09</td>
 +
  </tr> <tr>
 +
    <td>29/09
 +
    <ul>
 +
      <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
 +
                    <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
 +
                    <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>30/09
 +
    <ul>
 +
      <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
 +
                    <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
 +
                    <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
  </tr>
 +
</table>
 +
<br>
 +
<br>
 +
<br>
-
<!--requirements section -->
 
-
<tr><td colspan="3"> <h3>Notebook</h3></td></tr>
 
-
</tr>
 
-
<tr>
+
<thead><h2 align=center>October</h2>
-
<td width="45%"  valign="top">
+
-
<p>You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well. </p>
+
-
</td>
+
-
<td></td>
+
</thead>
-
<td></td>
+
-
</tr>
+
 +
<tbody>
 +
<table style="width:100%">
 +
  <tr>
 +
    <th style="background-color:#bfacc0">Monday</th>
 +
    <th style="background-color:#bfacc0">Tuesday</th>
 +
    <th style="background-color:#bfacc0">Wednesday</th>
 +
    <th style="background-color:#bfacc0">Thursday</th>
 +
    <th style="background-color:#bfacc0">Friday</th>
 +
    <th style="background-color:#bfacc0">Saturday</th>
 +
    <th style="background-color:#bfacc0">Sunday</th>
 +
  </tr> <tr>
 +
    <td></td>
 +
    <td></td>
 +
    <td>01/10
 +
    <ul>
 +
      <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
 +
                    <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
 +
                    <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
 +
      <ul>
 +
        <li>Glycerol Stock</li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>02/10</td>
 +
    <td>03/10</td>
 +
    <td>04/10</td>
 +
    <td>05/10</td>
 +
  </tr>
 +
<tr>
 +
    <td>06/10
 +
    <ul>
 +
      <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
 +
                    <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
 +
                    <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
 +
          <ahref="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>07/10 
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>08/10
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      </ul>
 +
    <ul>
 +
    </td>
 +
    <td>09/10
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
    </td>
 +
    <td>10/10
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>11/10</td>
 +
    <td>12/10</td>
 +
  </tr> <tr>
 +
    <td>13/10</td>
 +
    <td>14/10</td>
 +
    <td>15/10</td>
 +
    <td>16/10</td>
 +
    <td>17/10</td>
 +
    <td>18/10</td>
 +
    <td>19/10</td>
 +
  </tr> <tr>
 +
    <td>20/10</td>
 +
    <td>21/10</td>
 +
    <td>22/10</td>
 +
    <td>23/10</td>
 +
    <td>24/10</td>
 +
    <td>25/10</td>
 +
    <td>26/10</td>
 +
  </tr> <tr>
 +
    <td>27/10</td>
 +
    <td>28/10</td>
 +
    <td>29/10</td>
 +
    <td>30/10</td>
 +
    <td>31/10</td>
 +
    <td></td>
 +
    <td></td>
 +
  </tr>
</table>
</table>
-
<body>
 
-
  <h1> <strong>Lab Protocols</strong> </h1>
 
-
  <h2> Competent Cell with Calcium Chloride </h2>
 
-
      <ol>
 
-
        <li><h3>Materials</h3></li>
 
-
            <ul>
 
-
              <li> Solid LB medium - 1 plate </li>
 
-
              <li> Liquid LB medium </li>
 
-
              <li> 2 250ml centrifuge tubes </li>
 
-
              <li> Centrifuge </li>
 
-
              <li> Cell sample (DH5-alpha in our case) </li>
 
-
              <li> Autoclave </li>
 
-
              <li> Inoculation loop </li>
 
-
              <li> Liquid nitrogen </li>
 
-
              <li> 37°C oven </li>
 
-
              <li> CaCl<sub>2</sub>:</li>
 
-
                  <ul>
 
-
                    <li> 60mM CaCl<sub>2</sub> </li>
 
-
                    <li> 10mM HEPES </li>
 
-
                    <li> 15% glicerol </li>
 
-
                    <li> H<sub>2</sub>O to 100ml </li>
 
-
                  </ul>
 
-
            </ul>
 
-
        <li><h3> Methods </h3> </li>
 
-
        <p><strong>Day 1</strong></p>
 
-
            <ol>
 
-
              <li> Risk the LB plate with the cell sample (DH5-alpha) </li>
 
-
              <li> Incubate the plate at 37°C overnight </li>
 
-
            </ol>
 
-
        <p><strong>Day 2</strong></p>
 
-
            <ol>
 
-
              <li> Sterilize in the Autoclave </li>
 
-
              <ul>
 
-
                  <li> 100ml of liquid LB medium </li>
 
-
                  <li> 100 ml of CaCl<sub>2</sub>
 
-
                  <li> 2 250ml centrifuge tubes </li>
 
-
              </ul>
 
-
              <li> Prepare a 6ml LB inoculum with a DH5-alpha colony and incubate at 37°C/250rpm overnight</li>
 
-
            </ol>
 
-
        <p><strong>Day 3</strong></p>
 
-
            <ol>
 
-
              <li>Add 2ml of the inoculum in 100ml of liquid LB medium. Incubate at 37°C/250rpm until the OD reaches 0,375</li>
 
-
              <li> Distribute the volume in 2 250ml centrifuge tubes (pre chilled) and spin at 10000rpm/7min/4°C</li>
 
-
              <li> Discard the supernatant and ressuspend the pellet in 10ml of CaCl<sub>2</sub> solution</li>
 
-
              <li> Spin the tubes at 7000rpm/7min/4°C</li>
 
-
              <li> Discard the supernatant and ressuspend the pellet in 10ml of CaCl<sub>2</sub> solution. Incubate 30min on ice</li>
 
-
              <li> Spin at 7000rpm/7min/4°C</li>
 
-
              <li> Discard the supernatant and ressuspend the pellet in 2ml of the CaCl<sub>2</sub> solution</li>
 
-
              <li> Distribute the 2ml in 500ul tubes adding 50ul in each.</li>
 
-
              <li> Freeze the samples in liquid nitrogen </li>
 
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              <li> Stock at -80°C </li>
 
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            </ol>
 
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      </ol>
 
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<h2> Transformation in <em>Escherichia coli</em> (DH5-alpha)</h2>
 
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  <ol>
 
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      <li><h3> Materials</h3></li>
 
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        <ul>
 
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            <li> 1,5ml tube</li>
 
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            <li> Styrofoam box with ice</li>
 
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            <li> Agar plate with antibiotic </li>
 
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            <li> Competent cells </li>
 
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            <li> Plasmidial DNA </li>
 
-
            <li> Centrifuge </li>
 
-
            <li> Water bath at 42°C </li>
 
-
            <li> Liquid LB medium </li>       
 
-
            <li> Shaker </li>
 
-
        </ul>
 
-
      <li><h3>Methods</h3></li>
 
-
        <ol>
 
-
            <li>Briefly spin the competent cells and put then on ice </li>
 
-
            <li> Add 50ng of plasmidial DNA in a 1,5ml tube </li>
 
-
            <li> Add the 50ul of competent cell in the same tube</li>
 
-
            <li> Keep the tube on ice for 25min </li>
 
-
            <li> Put the tube in a 42° water bath for 2 min</li>
 
-
            <li> Put the tube on ice for 5 min</li>
 
-
            <li> Add 200ul of liquid LB </li>
 
-
            <li> Incubate at 250rpm/37°C/1 hour </li>
 
-
            <li> Plate the the solution in a Agar plate with the appropriate antibiotic </li>
 
-
            <li> Incubate the plate at 37°C overnight </li>
 
-
        </ol>
 
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  </ol>
 
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<h1>Assemblies</h1>
 
-
<!--estou colocando os fichamentos dos assemblies aqui, ainda preciso acabar os que faltam mas ja da pra experimentar com a organização destes-->
 
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  <ul>
 
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      <li><a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a></li>
 
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      <li><a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a></li>
 
-
  </ul>
 
</body>
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Latest revision as of 20:32, 17 October 2014

HeaderNotebookBRASILAP.png

Main Assembly Map

     The main assembly map describes all the constructions that would be done in the project, but some of them have not been performed in time of the iGEM deadline. The succesfully constructed assemblies is indicated by a green check point and the unsuccessfull constructions are indicated by a red "X". Click on these assemblies to access the lab form.

AI AII AIII BIII AIV BII CII DI AVI AV BIV

Assemblies forms

Assembly form template

This is the template designed to help with the laboratory organization during the assemble of biological parts. If you want to use the assembly method we used you can print the form and complete it for each construction. Hope it helps :)

Life Inside the LAB

July

Monday Tuesday Wednesday Thursday Friday Saturday Sunday
30/06
  • PCR lasR (BBa_C0079). The purpose of this PCR was to add the RBS (BBa_K143021), through addition of the sequence in the primer foward, and also to remove the LVA tag. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this part will be called BBa_K1521001.
 01/07 02/07 03/07 04/07 05/07 06/07
07/07 08/07 09/07 10/07
  • Miniprep, Quantification and Restriction Analysis:
    • BBa_B0015 (restriction analysis fail, repeat)
  • PCR the qteE gene for amplification. We also added the RBS (BBa_K143021,) using the foward primer. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this biobrick will be called BBa_K1521000.
  • Ligation of RBS+lasR in PTZ57R/T vector (instaclone kit)
 11/07 12/07 13/07
14/07 15/07 16/07 17/07 18/07 19/07 20/07
21/07 22/07 23/07 24/07 25/07 26/07 27/07
28/07
  • Assembly AIII:
    • Restriction analysis (+NdeI enzyme)
    • OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that both the construction and the pSB1C3 vector had similar size.
  • Assembly AII, AV and AVI:
 29/07 30/07 31/07
  • Assembly AII, AV and AVI:
    • Inoculum
  • PCR BBa_K143055 for RBS removal
  • OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this Lac promotor :)
  • PCR of Plac BBa_K143055 was followed by a Gel Purification and Ligation in the PUC19 vector



August

Monday Tuesday Wednesday Thursday Friday Saturday Sunday
01/08 02/08 03/08
04/08 05//08 06/08 07/08 08/08 09/08 10/08
11/08
  • Prepare Plac BBa_K143055 for sequencing
  • Analyse the RBS+qteE and RBS+lasR sequencing file
 12/08
  • Digestion:
    • RBS+qteE
    • RBS+lasR
    • OBS: this digestion was performed so we clone this parts in the pSB1C3 vector to put them in Biobrick standard
 13/08 14/08
  • Gel Purification and Ligation in pSB1C3 for BBiobrick standard:
    • RBS+qteE
    • RBS+lasR
  • Assemblies BII and BIII:
    • Gel Purification
    • OBS: we had some problems here with the purification and we lost all our digestions, so we're repeating the digestion
 15/08 16/08 17/08
18/08 19/08 20/08
  • RBS+qteE, RBS+lasR and Assemblies BII and BIII:
    • Inoculum
 21/08 22/08 23/08 24/08
25/08 26/08 27/08 28/08 29/08 30/08 31/08



September

Monday Tuesday Wednesday Thursday Friday Saturday Sunday
01/09 02/09 03/09 04/09 05/09 06/09 07/09
08/09 09/09 10/09 11/09 12/09 13/09
  • Assembly KX
    • Inoculum
  • Assemblies DI, KVIII and KXI:
  • Prepare LB medium (solid and liquid)
 14/09
15/09 16/09 17/09
  • Measurement Interlab Study - Samples growth biobrick devices 1, 2 and 3
 18/09
  • Measurement Interlab Study - Sample preparation and characterization by Flow Cytometry and Fluorometry
 19/09 20/09 21/09
22/09 23/09 24/09 25/09
  • Assembly KXIV:
    • Inoculum
 26/09 27/09 28/09
29/09 30/09



October

Monday Tuesday Wednesday Thursday Friday Saturday Sunday
01/10 02/10 03/10 04/10 05/10
06/10 07/10 08/10 09/10
  • Assemblies KIX, BIV and KXVI
    • Inoculum
    		 10/10 11/10 12/10
    13/10 14/10 15/10 16/10 17/10 18/10 19/10
    20/10 21/10 22/10 23/10 24/10 25/10 26/10
    27/10 28/10 29/10 30/10 31/10

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