Team:TCU Taiwan/Attributions

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     <td width="0%">&nbsp;</td>
     <td width="0%">&nbsp;</td>
     <td width="128" height="128" align="left" valign="middle"><img src="https://static.igem.org/mediawiki/2014/5/51/TCU-Attributions-logo.png" width="128" height="128"></td>
     <td width="128" height="128" align="left" valign="middle"><img src="https://static.igem.org/mediawiki/2014/5/51/TCU-Attributions-logo.png" width="128" height="128"></td>
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     <td width="68%" align="left" valign="middle"><font face="Trebuchet MS" size="7">Attributions</font></td>
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     <td width="68%" align="left" valign="middle"><h6><font face="Trebuchet MS" size="7" color="#484644">Attributions</font></h6></td>
     <td width="19%" align="center"><img src="https://static.igem.org/mediawiki/2014/c/c2/TCU-Logo-up.png" width="90%"></td>
     <td width="19%" align="center"><img src="https://static.igem.org/mediawiki/2014/c/c2/TCU-Logo-up.png" width="90%"></td>
     <td width="0%">&nbsp;</td>
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<li><a href="#" style="cursor:default"><font size="5">Team</font></a>
<li><a href="#" style="cursor:default"><font size="5">Team</font></a>
     <ul>
     <ul>
-
       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Team">Team Members</a></li>
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       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Team"><font size="3">Members</font></a></li>
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       <li><a href="https://igem.org/Team.cgi?year=2014">Official Team Profile</a></li>
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      <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Achievements"><font size="3">Achievements</font></a></li>
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       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Contact">Contact Us</a></li>
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       <li><a href="https://igem.org/Team.cgi?id=1473" target="_blank"><font size="3">Official Team Profile</font></a></li>
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       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Contact"><font size="3">Contact</font></a></li>
</ul>
</ul>
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   <li><a href="#" style="cursor:default"><font size="5">Project</font></a>
   <li><a href="#" style="cursor:default"><font size="5">Project</font></a>
     <ul>
     <ul>
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       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Project">Project</a></li>
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       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Project"><font size="3">Introduction</font></a></li>
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       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Parts">Parts</a></li>
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       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Parts"><font size="3">Parts</font></a></li>
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       <li><a href="">Human Pratics</a></li>
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       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/SystemDesign"><font size="3">System Design</font></a></li>
 +
      <li><a href="https://2014.igem.org/Team:TCU_Taiwan/M13Phage"><font size="3">M13 Phage</font></a></li>
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      <li><a href="https://2014.igem.org/Team:TCU_Taiwan/CRISPR"><font size="3">CRISPR</font></a></li>
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      <li><a href="https://2014.igem.org/Team:TCU_Taiwan/FutureWorks"><font size="3">Future Works</font></a></li>
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      <li><a href="https://2014.igem.org/Team:TCU_Taiwan/HumanPratics"><font size="3">Human Pratics</font></a></li>
     </ul>
     </ul>
   </li>
   </li>
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<!--Attributions content -->
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<tr><td > <h3> iGEM Team attributions page</h3></td>
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<td > <h3> </h3></td>
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    <td align="justify"><font size="5" face="Verdana" color="#D92332">We would like to thank all the people who came to our progress presentations and gave useful feedback.</font></td>
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  </tr>
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        <tr>
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Each team must clearly attribute work done by the student team members on this pageThe team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster.
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          <td height="30px">&nbsp;</td>
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          <td>
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          <table width="100%" border="0" cellspacing="0" cellpadding="0">
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              <td bgcolor="#FFF2B5height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">
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Instructors support</font></td>
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            </tr>
 +
            <tr>
 +
              <td height="20px" colspan="2">&nbsp;</td>
 +
            </tr>
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            <tr>
 +
              <td height="20px" colspan="2">
 +
              <table width="90%" border="0" cellspacing="0" cellpadding="0" align="center">
 +
                <tr>
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                  <td>
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                  <fieldset>
 +
<legend><font face="Trebuchet MS" size="6" color="#fff">Lin,Guang-Huey</font></legend>
 +
                <p align="justify"><img src="https://static.igem.org/mediawiki/2014/6/65/TCU_%E6%9E%97%E5%85%89%E6%85%A7.jpg" width="120" height="150" style="float: left; margin-right: 5px;"><font face="Trebuchet MS" size="4" color="#484644">Professor Lin teaches knowledge about microbiology for TCU iGEM team each year, and she also guides our experimental design. Besides, she provides her lab for us to working on our experiment.</font></p>
 +
</fieldset>
 +
                </td>
 +
                  <td width="15">&nbsp;</td>
 +
                  <td>
 +
                  <fieldset>
 +
<legend><font face="Trebuchet MS" size="6" color="#fff">Lin,Nien-Tsung</font></legend>
 +
                <p align="justify"><img src="https://static.igem.org/mediawiki/2014/f/f8/TCU_%E6%9E%97%E5%BF%B5%E7%92%81.jpg" width="120" height="150" style="float: left; margin-right: 5px;"><font face="Trebuchet MS" size="4" color="#484644">Prof. Lin, a gracious teacher, is the guiders of our experiment on M13 phage display. She provides us a lot knowledge about M13 phage and DNA cloning.</font></p>
 +
</fieldset>
 +
                </td>
 +
                </tr>
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                <tr>
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                  <td height="15">&nbsp;</td>
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                  <td>&nbsp;</td>
 +
                  <td>&nbsp;</td>
 +
                </tr>
 +
                <tr>
 +
                  <td><fieldset>
 +
<legend><font face="Trebuchet MS" size="6" color="#fff">Ching,Yung-Hao</font></legend>
 +
                <p align="justify"><img src="https://static.igem.org/mediawiki/2014/1/1c/TCU_%E9%9D%96%E6%B0%B8%E7%9A%93.jpg" width="129" height="177" style="float: left; margin-right: 5px;"><font face="Trebuchet MS" size="4" color="#484644">Prof. Ching is one of the leader teachers of our team.
 +
She is a kind teacher who always encourages us, and she also gives us much advice and direction on safety concerns. </font></p>
 +
</fieldset></td>
 +
                  <td>&nbsp;</td>
 +
                  <td><fieldset>
 +
<legend><font face="Trebuchet MS" size="6" color="#fff">Woon,Peng-Yeong</font></legend>
 +
                <p align="justify"><img src="https://static.igem.org/mediawiki/2014/2/2b/TCU_%E6%BA%AB%E7%A7%89%E7%A5%A5.jpg" width="125" height="150" style="float: left; margin-right: 5px;"><font face="Trebuchet MS" size="4" color="#484644">Prof. Woon is one of the leader teachers for this year. He helps us the basic concept of iGEM and reminds us many iGEM rules or requests we nearly missed. He is a lively professor.</font></p>
 +
</fieldset></td>
 +
                </tr>
 +
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 +
            </tr>
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            <tr>
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              <td width="50%" height="30px">&nbsp;</td>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
          </table>
 +
          <table width="100%" border="0" cellspacing="0" cellpadding="0">
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            <tr>
 +
              <td bgcolor="#FFF2B5"  height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">Experiment</font></td>
 +
            </tr>
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            <tr>
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              <td height="20px" colspan="2">&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td colspan="2"><font size="3" face="Verdana" color="#0092C7">Bobo, Regina, Denise, Eating</font>
 +
</td>
 +
            </tr>
 +
            <tr>
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              <td colspan="2" height="20px">&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td colspan="2"><font face="Trebuchet MS" size="5" color="#90B849">Contributions</font></td>
 +
            </tr>
 +
            <tr>
 +
              <td colspan="2" height="10px">&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td width="50%" valign="top"><font size="3" face="Verdana" color="#333"><li>Transformation ( Cas9 in  pSB1C3 into DH5α)<br>
 +
                <li>Extract plasmid with pSB1C3  from DH5α.<br>
 +
                <li>Extract plasmid pSB1C3 with BBa_I13521、BBa_ K914003、BBa_ K1218011.<br>
 +
                <li>Use Enzyme digestion (<em>Pst</em>I and <em>EcoR</em>I) to confirm whether pSB1C3 and pBluescript is successfully  extracted.<br>
 +
                <li>Purification of RFP sequence from pSB1C3 <li>Prepare Cm. plate、LB. plate.<br>
 +
                <li>Ligation of RFP and pBluescript SK(-).<br>
 +
                <li>Transformation clone RFP/pBluescript SK(-) to DH5α.<br>
 +
                <li>Extract plasmid clone RFP/pBluescript SK(-).</li></font></td>
 +
              <td rowspan="3"><p><img src="https://static.igem.org/mediawiki/2014/f/ff/TCU_TEAM_BO_n.jpg" style="height: 150px; float: left; margin-right: 20px; margin-left: 30px; margin-bottom:10px"><img src="https://static.igem.org/mediawiki/2014/7/7f/TCU_TEAM_RE_n.jpg" style="height: 150px; float: left; margin-right: 20px; margin-left: 30px; margin-bottom:10px"></p><p><img src="https://static.igem.org/mediawiki/2014/3/3d/TCU_TEAM_REN_n.jpg" style="height: 150px; float: right; margin-right: 30px; margin-left: 20px; margin-bottom:30px"><img src="https://static.igem.org/mediawiki/2014/3/31/TCU_TEAM_n.jpg" style="height: 150px; float: right; margin-right: 30px; margin-left: 20px; margin-bottom:30px"></p>
 +
              <p align="center"><img src="https://static.igem.org/mediawiki/2014/e/ec/TCU_ATT_10726611_854696624548888_1884588712_n.jpg" width="50%"></p></td>
 +
            </tr>
 +
            <tr>
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              <td>
 +
              <fieldset>
 +
<legend><font face="Trebuchet MS" size="4" color="#fff">Experiment Design</font></legend>
 +
                <p><font size="3" face="Verdana" color="#90B849">Ted and                  Bobo </font><font size="3" face="Verdana" color="#90B849"><br><br>
 +
                </font><font size="3" face="Verdana" color="#333">
 +
Design gRNA for AmpR.
 +
                </font></p>
 +
</fieldset>
 +
</td>
 +
            </tr>
 +
            <tr>
 +
              <td><fieldset>
 +
<legend><font face="Trebuchet MS" size="4" color="#fff">Biobricks Design</font></legend>
 +
                <p><font size="3" face="Verdana" color="#90B849">Ted</font><font size="3" face="Verdana" color="#90B849"><br><br>
 +
                </font><font size="3" face="Verdana" color="#333">
 +
Design the gRNA for different resistance gene.
 +
                </font></p>
 +
</fieldset></td>
 +
            </tr>
 +
            <tr>
 +
              <td height="30px">&nbsp;</td>
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              <td>&nbsp;</td>
 +
            </tr>
 +
          </table></td>
 +
        </tr>
 +
        <tr>
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          <td> <table width="100%" border="0" cellspacing="0" cellpadding="0">
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              <td bgcolor="#FFF2B5"  height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">Modeling</font></td>
 +
            </tr>
 +
            <tr>
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              <td height="10px" colspan="2">&nbsp;</td>
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            </tr>
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            <tr>
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              <td colspan="2"><font size="3" face="Verdana" color="#0092C7">House, Bobo, Chao-Di Chang(National Chiao Tung University)</font></td>
 +
            </tr>
 +
            <tr>
 +
              <td height="10px" colspan="2">&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td colspan="2"><font face="Trebuchet MS" size="5" color="#90B849">Contributions</font></td>
 +
            </tr>
 +
            <tr>
 +
              <td colspan="2" height="10px">&nbsp;</td>
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            </tr>
 +
            <tr>
 +
              <td width="50%" valign="top"><font size="3" face="Verdana" color="#333"><li>Infectional efficiency ratio of  M13KO7.<br>
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                <li> Release titer.(Releasing titer of phagemid which is <em>E.coli</em> infected by helper phage)<br>
 +
                </font></td>
 +
              <td rowspan="4"><p align="center"><img src="https://static.igem.org/mediawiki/2014/1/10/TCU_ATT_MODEL_10728809_854696611215556_2059562591_n.jpg" width="80%"></p>
 +
              </td>
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            </tr>
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            <tr>
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              <td valign="top"><fieldset>
 +
<legend><font face="Trebuchet MS" size="4" color="#fff">Special Thanks</font></legend>
 +
                <p align="justify"><img src="https://static.igem.org/mediawiki/2014/1/14/TCU_TEAM_SUPo.jpg" width="204" height="200" style="float: right; margin-right: 5px;margin-left: 5px;""><font face="Trebuchet MS" size="4" color="#90B849"><p>Modeling Advisor</p></font><font face="Trebuchet MS" size="4" color="#A66B38"><p>Chang,Chao-Di</p></font><br>
 +
                <font face="Trebuchet MS" size="4" color="#333"><p>Thanks for Chang,Chao-Di strong support on our modeling.</p></font>
 +
  </fieldset></td>
 +
            </tr>
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            <tr>
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              <td>
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</td>
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              <td></td>
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            </tr>
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            <tr>
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              <td height="30px">&nbsp;</td>
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              <td>&nbsp;</td>
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            </tr>
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          </table></td>
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        </tr>
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    </table>
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            <tr>
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              <td bgcolor="#FFF2B5"  height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">Human Practice</font></td>
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            </tr>
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            <tr>
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              <td height="20px" colspan="2">&nbsp;</td>
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            </tr>
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              <td colspan="2"><font size="3" face="Verdana" color="#0092C7">Eating</font>
</td>
</td>
 +
            </tr>
 +
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              <td colspan="2" height="20px">&nbsp;</td>
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            </tr>
 +
            <tr>
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              <td colspan="2"><font face="Trebuchet MS" size="5" color="#90B849">Contributions</font></td>
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            </tr>
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            <tr>
 +
              <td colspan="2" height="10px">&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td width="50%" valign="top"><font size="3" face="Verdana" color="#333"><li>Held the 2014 Synthetic Biology information meeting of local senior high school and Tzu Chi University as a chief convener.<br>
 +
                <li>Contact with National Chiao Tung University for cooperation.<br></font></td>
 +
              <td rowspan="3"> </td>
 +
            </tr>
 +
            <tr>
 +
              <td>
 +
             
 +
</td>
 +
            </tr>
 +
            <tr>
 +
              <td></td>
 +
            </tr>
 +
            <tr>
 +
              <td height="30px">&nbsp;</td>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
          </table>
 +
          <table width="100%" border="0" cellspacing="0" cellpadding="0">
 +
            <tr>
 +
              <td bgcolor="#FFF2B5"  height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">Wiki</font></td>
 +
            </tr>
 +
            <tr>
 +
              <td height="20px" colspan="2">&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td colspan="2"><font size="3" face="Verdana" color="#0092C7">House</font>
 +
</td>
 +
            </tr>
 +
            <tr>
 +
              <td colspan="2" height="20px">&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td colspan="2"><font face="Trebuchet MS" size="5" color="#90B849">Contributions</font></td>
 +
            </tr>
 +
            <tr>
 +
              <td colspan="2" height="10px">&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td width="50%" valign="top"><font size="3" face="Verdana" color="#333"><li>Construct the wiki page.<br>
 +
                <li>Coding,coding and coding the wiki page.<br> <li>Study all about Synthetic Biology from wetlab to drylab.</font></td>
 +
              <td rowspan="3"><table width="80%" border="0" cellspacing="0" cellpadding="0" align="center">
 +
                <tr>
 +
                  <td><img src="https://static.igem.org/mediawiki/2014/a/af/TCU_ATT_HOU_10742613_948400915176300_1493079925_o.jpg" width="100%"></td>
 +
                </tr>
 +
              </table></td>
 +
            </tr>
 +
            <tr>
 +
              <td>
 +
             
 +
</td>
 +
            </tr>
 +
            <tr>
 +
              <td></td>
 +
            </tr>
 +
            <tr>
 +
              <td height="30"></td>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
          </table>
 +
          <table width="100%" border="0" cellspacing="0" cellpadding="0">
 +
            <tr>
 +
              <td bgcolor="#FFF2B5"  height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">And last but not least</font></td>
 +
            </tr>
 +
            <tr>
 +
              <td height="20px" colspan="2">
 +
              <table width="90%" border="0" cellspacing="0" cellpadding="0" align="center">
 +
                <tr>
 +
                  <td width="48%">
 +
                  <fieldset>
 +
<legend><font face="Trebuchet MS" size="4" color="#fff">Art Design</font></legend>
 +
                <p><font size="3" face="Verdana" color="#90B849">Denise, Regina, Eating, House</font><font size="3" face="Verdana" color="#90B849"><br><br>
 +
                </font><font size="3" face="Verdana" color="#333">Design of poster and PPT.
 +
<br>
 +
Design of team uniform.
 +
                </font></p>
 +
</fieldset>
 +
                </td>
 +
                  <td width="10px">&nbsp;</td>
 +
                  <td>
 +
                    <fieldset>
 +
                      <legend><font face="Trebuchet MS" size="4" color="#fff">Photo & Video</font></legend>
 +
                      <p><font size="3" face="Verdana" color="#90B849">Sharon, Eating</font><font size="3" face="Verdana" color="#90B849"><br><br>
 +
                        </font><font size="3" face="Verdana" color="#333"> Take photo and video.
 +
  <br>Arrange all photos.
 +
                         
 +
                        </font></p>
 +
                      </fieldset>
 +
                  </td>
 +
                  </tr>
 +
                <tr>
 +
                  <td><fieldset>
 +
                      <legend><font face="Trebuchet MS" size="4" color="#fff">Administeration</font></legend>
 +
                      <p><font size="3" face="Verdana" color="#90B849">Eating</font><font size="3" face="Verdana" color="#90B849"><br><br>
 +
                        </font><font size="3" face="Verdana" color="#333">Apply for the allowances.<br>Arrange all files.</font></p>
 +
                      </fieldset></td>
 +
                  <td>&nbsp;</td>
 +
                  <td></td>
 +
                </tr>
 +
              </table></td>
 +
            </tr>
 +
            <tr>
 +
              <td width="50%" colspan="2"></td>
 +
            </tr>
 +
            <tr>
 +
              <td>
 +
             
 +
</td>
 +
            </tr>
 +
            <tr>
 +
              <td></td>
 +
            </tr>
 +
            <tr>
 +
              <td height="30px">&nbsp;</td>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
          </table>
 +
          <table width="100%" border="0" cellspacing="0" cellpadding="0">
 +
            <tr>
 +
              <td bgcolor="#FFF2B5"  height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">Video support</font></td>
 +
            </tr>
 +
            <tr>
 +
              <td height="20px" colspan="2">&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td colspan="2"><font size="3" face="Verdana" color="#0092C7">Wang, Yiru and Chen, Jiawei</font>
 +
</td>
 +
            </tr>
 +
            <tr>
 +
              <td colspan="2" height="20px">&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td colspan="2"><font face="Trebuchet MS" size="3" color="#90B849">we should be obliged to them for their assistance in this matter.</font></td>
 +
            </tr>
 +
            <tr>
 +
              <td colspan="2" height="10px">&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td width="50%" align="center"><p><img src="https://static.igem.org/mediawiki/2014/6/66/TCU_Yiru.jpg" width="50%"></p>
 +
              <p><font size="3" face="Verdana" color="#333">Wang, Yi-ru</font></p></td>
 +
              <td rowspan="3" align="center"><p><img src="https://static.igem.org/mediawiki/2014/7/75/TCU_Jiawei.jpg" width="50%"></p>
 +
              <p><font size="3" face="Verdana" color="#333">Chen, Jia-wei</font></p></td>
 +
            </tr>
 +
            <tr>
 +
              <td>
 +
             
 +
</td>
 +
            </tr>
 +
            <tr>
 +
              <td></td>
 +
            </tr>
 +
            <tr>
 +
              <td height="30px">&nbsp;</td>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
          </table>
 +
          <table width="100%" border="0" cellspacing="0" cellpadding="0">
 +
            <tr>
 +
              <td bgcolor="#FFF2B5"  height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">Special Thanks</font></td>
 +
            </tr>
 +
            <tr>
 +
              <td height="20px" colspan="2">&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td colspan="2"><font size="3" face="Verdana" color="#0092C7">Our partner : <a style="text-decoration: none; color:#0092C7" href="https://2014.igem.org/Team:NCTU_Formosa" target="_blank">NCTU_Formosa</a></font>
 +
</td>
 +
            </tr>
-
<tr>
+
            <tr>
-
<td > <h3> Why do we have this requirement?</h3></td>
+
              <td colspan="2" height="10px">&nbsp;</td>
-
<td></td>
+
            </tr>
-
<td > <h3>Attribution Template </h3></td>  
+
            <tr>
 +
              <td width="50%" valign="top"><font size="3" face="Verdana" color="#333">
 +
                <li>We are extremely appreciative of NCTU_Formosa that helped and support us.<br>
 +
                <li>The model1 courtesy of <a href="https://2014.igem.org/Team:NCTU_Formosa/team#CHAO-DI_CHANG" target="_blank">Chao-Di Chang.</a><img src="https://static.igem.org/mediawiki/2014/7/7f/TCU_HP_NJTU.jpg" width="70%"><br></font></td>
 +
              <td rowspan="3" align="center"><a href="https://2014.igem.org/Team:NCTU_Formosa" target="_blank" title="NCTU_Formosa Wiki"><img src="https://static.igem.org/mediawiki/2014/0/09/FINAL.gif" width="50%"></td>
 +
            </tr>
 +
            <tr>
 +
              <td>
 +
             
 +
</td>
 +
            </tr>
 +
            <tr>
 +
              <td></td>
 +
            </tr>
 +
            <tr>
 +
              <td height="30px">&nbsp;</td>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td bgcolor="#FFF2B5"  height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">Sponsors</font></td>
 +
            </tr>
 +
            <tr>
 +
              <td height="10px" colspan="2">&nbsp;</td>
 +
            </tr>
 +
          </table>
 +
         
 +
          <table width="100%" border="0" cellspacing="0" cellpadding="0">
 +
  <tr>
 +
    <td align="center">
 +
   
 +
    <!--3-->
 +
    <a href="http://english.moe.gov.tw/" title="Ministry of Education,Republic of China" target="_blank"><img src="https://static.igem.org/mediawiki/2014/8/83/TCU_Moe_logo_BLACK.png" height="100" onMouseMove="this.src=Image3.src" onMouseOut="this.src='https://static.igem.org/mediawiki/2014/8/83/TCU_Moe_logo_BLACK.png'"></a>&nbsp;&nbsp;&nbsp;
 +
   
 +
    <!--1-->
 +
    <a href="http://tec.tcu.edu.tw/100/" title="Center of Teaching Excellence,TCU" target="_blank"><img src="https://static.igem.org/mediawiki/2014/2/26/TCU_TEACH_BLACK.png" height="100" onMouseMove="this.src=Image1.src" onMouseOut="this.src='https://static.igem.org/mediawiki/2014/2/26/TCU_TEACH_BLACK.png'"></a>&nbsp;&nbsp;
 +
   
 +
    <!--5-->
 +
    <a href="http://www.tzuchi.org/" title="Buddhist Compassion Relief Tzu Chi Foundation" target="_blank"><img src="https://static.igem.org/mediawiki/2014/f/ff/TZUCHI_SUP_BLACK.png" height="90" onMouseMove="this.src=Image5.src" onMouseOut="this.src='https://static.igem.org/mediawiki/2014/f/ff/TZUCHI_SUP_BLACK.png'"></a>&nbsp;&nbsp;
 +
   
 +
    <!--4-->
 +
    <a href="http://eng.tcu.edu.tw/" title="Tzu Chi University" target="_blank"><img src="https://static.igem.org/mediawiki/2014/4/43/TCU_SUP_LOGO_BLACK.png" height="90" onMouseMove="this.src=Image4.src" onMouseOut="this.src='https://static.igem.org/mediawiki/2014/4/43/TCU_SUP_LOGO_BLACK.png'"></a>&nbsp;
 +
 
 +
    <!--2-->
 +
    <a href="https://2013.igem.org/Team:TzuChiU_Formosa" title="2013 TzuChiU_Formosa" target="_blank"><img src="https://static.igem.org/mediawiki/2014/7/75/2013_TCUIGEM_BLACK.png" height="130" onMouseMove="this.src=Image2.src" onMouseOut="this.src='https://static.igem.org/mediawiki/2014/7/75/2013_TCUIGEM_BLACK.png'"></a>
 +
   
 +
   
 +
   
 +
   
 +
   
 +
  </td>
   </tr>
   </tr>
 +
</table>
 +
<table width="100%" border="0" cellspacing="0" cellpadding="0">
 +
            <tr>
 +
              <td bgcolor="#FFF2B5"  height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">General support</font></td>
 +
            </tr>
 +
            <tr>
 +
              <td height="20px" colspan="2">&nbsp;</td>
 +
            </tr>
-
<tr>
+
            <tr>
 +
              <td colspan="2" height="10px">
 +
              <table width="600" height="90" border="0" cellspacing="0" cellpadding="0" align="center" style="background-color:#01395C">
 +
  <tr>
 +
    <td><a href="http://www.microbiology.tcu.edu.tw/english/home.html" title="Master program in Microbiology and Immunology" target="_blank"><img src="http://www.microbiology.tcu.edu.tw/images/logo/logo_v2.png" alt="Master program in Microbiology and Immunology" width="600" height="90"></a>
 +
    </td>
 +
  </tr>
 +
</table>
 +
</td>
 +
            </tr>
 +
            <tr>
 +
              <td colspan="2" height="15px">&nbsp;</td>
 +
            </tr>
 +
            <tr>
 +
              <td colspan="2" height="10px">
 +
              <table width="600" height="90" border="0" cellspacing="0" cellpadding="0" align="center" style="background-color:#000000">
 +
  <tr>
 +
    <td><a href="http://www.mi.tcu.edu.tw/english_web/introduction.htm" title="Department of Medical Informatics" target="_blank"><img src="http://www.mitest.tcu.edu.tw/images/logo/logo_v3.png" alt="Department of Medical Informatics" width="550" height="100"></a>
 +
    </td>
 +
  </tr>
 +
</table>
 +
</td>
 +
            </tr>
 +
              <td colspan="2" height="15px">&nbsp;</td>
 +
            </tr>
 +
          <tr>
 +
              <td colspan="2" height="10px">
 +
              <table width="600" height="90" border="0" cellspacing="0" cellpadding="0" align="center">
 +
  <tr>
 +
    <td><a href="http://www.dmbhg.tcu.edu.tw/dmbhg.html" title="Department of Molecular Biology and Human Genetics" target="_blank"><img src="http://www.dmbhg.tcu.edu.tw/image/dLogo.jpg" alt="Department of Molecular Biology and Human Genetics" width="100%"></a></td>
 +
  </tr>
 +
</table>
 +
</td>
 +
            </tr>
 +
            <tr>
 +
              <td width="50%" height="50px">&nbsp;</td>
 +
              <td>&nbsp;</td>
 +
            </tr>
 +
          </table>
 +
    </td>
 +
</tr>
 +
<tr> <td  height="5px"> </td></tr>
-
<td width="45%"  valign="top">
+
<!--Attributions content -->
-
<p>
+
</table>
-
We have this requirement to help the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, just be sure to report the work your team did and the work that was done by others.  
+
<table width="70%" border="0" cellspacing="0" cellpadding="0" align="center">
-
</p>
+
  <tr>
 +
    <td><p align="center">&nbsp;</p>
 +
      <p align="center"><font size="7" face="Verdana" color="#D92332">Acknowledgements</font></p></td>
 +
  </tr>
 +
</table>
 +
<table width="50%" border="0" cellspacing="0" cellpadding="0" align="center" height="200">
 +
  <tr>
 +
    <td valign="top"><p align="center">&nbsp;</p>
 +
      <p><font size="5" face="Verdana" color="#333">We have taken efforts in this project. However, it would not have been possible without the kind support and help of many individuals and organizations. We would like to extend our sincere thanks to all of them.</font></p></td>
 +
  </tr>
 +
</table>
-
<p>
+
<table width="100%" border="0" cellspacing="0" cellpadding="0">
-
For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is something that is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.  
+
  <tr>
-
</p>
+
    <td><img src="https://static.igem.org/mediawiki/2014/c/c1/TCU-Line.png" width="100%"></td>
 +
  </tr>
 +
</table>
 +
<table width="100%" border="0" align="center" style="background-color:#F2DE79" height="150px">
 +
  <tr align="center" bgcolor="#F2DE79" height="150px" style="width:150px">
 +
    <td width="15%" align="center"><div id="gotop">^</div>
 +
    <a href="https://2014.igem.org/Team:TCU_Taiwan/Contact" title="Contact Us"><img src="https://static.igem.org/mediawiki/2014/8/84/TCU-Contact-bot.png" width="80" onMouseMove="this.src=Image0.src;" onMouseOut="this.src='https://static.igem.org/mediawiki/2014/8/84/TCU-Contact-bot.png'"></a>
 +
    <br><br>
 +
    <a href="mailto: tcutaiwan@gmail.com" target="_blank" title="Gmail"><img src="https://static.igem.org/mediawiki/2014/c/c4/TCU%E3%84%A6Gmail.png" width="30"></a>&nbsp;
 +
    <a href="https://www.facebook.com/TCUiGEM?ref=ts&fref=ts"  target="_blank" title="Facebook"><img src="https://static.igem.org/mediawiki/2014/b/b9/TCU-Facebook.png" width="30"></a>&nbsp;<a href="https://www.youtube.com/channel/UCr40ZmaFNJGx7tUufCXK_OQ" target="_blank" title="YouTube"><img src="https://static.igem.org/mediawiki/2014/2/29/TCU_Youtube.png" width="30"></a>&nbsp;<a href="http://tcuigem.wordpress.com/" target="_blank" title="Wordpress"><img src="https://static.igem.org/mediawiki/2014/f/f0/TCU-Wordpress.png" width="30"></a>
 +
    </td>
 +
    <td width="0.5px" align="center" bgcolor="#A66B38"> </td>
 +
    <td>
-
<p>
+
      <table width="70%" border="0" cellspacing="0" cellpadding="0" align="center" style="background-color:#F2DE79">
-
A great example of complete attribution comes from the <a href="https://2011.igem.org/Team:Imperial_College_London/Team">Imperial College London 2011 team</a> (scroll down to the bottom of their team page to see attributions).
+
        <tr align="center">
-
</p>
+
          <td><font size="2" face="Verdana" color="#F2DE79" id="Members">Team Members</font></td>
-
 
+
          <td><font size="2" face="Verdana" color="#F2DE79" id="Project">Project</font></td>
-
  </td>
+
          <td><font size="2" face="Verdana" color="#F2DE79" id="Parts">Parts</font></td>
-
<td></td>
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          <td><font size="2" face="Verdana" color="#F2DE79" id="Pratics">Human Pratics</font></td>
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<td width="45%" valign="top">  
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          <td><font size="2" face="Verdana" color="#F2DE79" id="Modeling">Modeling</font></td>
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<p>
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          <td><font size="2" face="Verdana" color="#F2DE79" id="Safety">Safety</font></td>
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Here are some of the fields we recommend you have on this page. If there are other areas not listed below, but applicable to your team/project, please feel free to also list them on your attributions page. Please feel free to remove any areas not applicable to your project.  
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          <td><font size="2" face="Verdana" color="#F2DE79" id="Notebook">Notebook</font></td>
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</p>
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          <td><font size="2" face="Verdana" color="#F2DE79" id="Attributions">Attributions</font></td>
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        </tr>
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<ol>
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        <tr>
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<li>General Support</li>
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          <td><a href="https://2014.igem.org/Team:TCU_Taiwan/Team" title="Team Members"><img src="https://static.igem.org/mediawiki/2014/1/1a/TCU-Team-logo.png" width="100"onMouseOver="this.width=this.width*1.1;Members.color='#333'" onMouseOut="this.width=this.width/1.1;Members.color='#F2DE79'"></a></td>
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          <td><a href="https://2014.igem.org/Team:TCU_Taiwan/Project" title="Project"><img src="https://static.igem.org/mediawiki/2014/f/f4/TCU-Project-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Project.color='#333'" onMouseOut="this.width=this.width/1.1;Project.color='#F2DE79'"></a></td>
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<li>Project support and advice</li>
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          <td><a href="https://2014.igem.org/Team:TCU_Taiwan/Parts" title="Parts"><img src="https://static.igem.org/mediawiki/2014/e/e5/TCU-Part-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Parts.color='#333'" onMouseOut="this.width=this.width/1.1;Parts.color='#F2DE79'"></a></td>
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          <td><a href="https://2014.igem.org/Team:TCU_Taiwan/HumanPratics" title="Human Pratics"><img src="https://static.igem.org/mediawiki/2014/d/dd/TCU-Human-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Pratics.color='#333'" onMouseOut="this.width=this.width/1.1;Pratics.color='#F2DE79'"></a></td>
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<li>Fundraising help and advice</li>
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          <td><a href="https://2014.igem.org/Team:TCU_Taiwan/Modeling" title="Modeling"><img src="https://static.igem.org/mediawiki/2014/3/3f/TCU-Modeling-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Modeling.color='#333'" onMouseOut="this.width=this.width/1.1;Modeling.color='#F2DE79'"></a></td>
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          <td><a href="https://2014.igem.org/Team:TCU_Taiwan/Safety" title="Safety"><img src="https://static.igem.org/mediawiki/2014/2/21/TCU-Safety-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Safety.color='#333'" onMouseOut="this.width=this.width/1.1;Safety.color='#F2DE79'"></a></td>
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<li>Lab support</li>
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          <td><a href="https://2014.igem.org/Team:TCU_Taiwan/Notebook" title="Notebook"><img src="https://static.igem.org/mediawiki/2014/2/25/TCU-Notebook-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Notebook.color='#333'" onMouseOut="this.width=this.width/1.1;Notebook.color='#F2DE79'"></a></td>
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          <td><a href="https://2014.igem.org/Team:TCU_Taiwan/Attributions" title="Attributions"><img src="https://static.igem.org/mediawiki/2014/5/51/TCU-Attributions-logo.png" width="100" onMouseOver="this.width=this.width*1.1;Attributions.color='#333'" onMouseOut="this.width=this.width/1.1;Attributions.color='#F2DE79'"></a></td>
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<li>Difficult technique support</li>
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        </tr>
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      </table>
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<li>Project advisor support</li>
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      <p><font size="2" face="Verdana" color="#333" id="tip">Lost the way? Use it to help you if you're lost</font><font color="#333" size="2" face="Verdana">.</font></p>
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    </td>
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<li>Wiki support</li>
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    <td width="1px" align="center" bgcolor="#A66B38"> </td>
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    <td width="15%" align="center"><p><a href="https://2014.igem.org/Team:TCU_Taiwan" title="Home"><img src="https://static.igem.org/mediawiki/2014/c/c3/TCU-Logo-d.png" width="100"></a></p>
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<li>Presentation coaching</li>
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    <p><img src="http://i.creativecommons.org/l/by/3.0/88x31.png" width="100"></p>      <p></p></td>
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  </tr>
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<li>Policy & Practices support</li>
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<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team.</li>
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</ol>
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</td>
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</table>
</table>
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Latest revision as of 22:08, 17 October 2014


 
Attributions
 
We would like to thank all the people who came to our progress presentations and gave useful feedback.
 
Instructors support
 
Lin,Guang-Huey

Professor Lin teaches knowledge about microbiology for TCU iGEM team each year, and she also guides our experimental design. Besides, she provides her lab for us to working on our experiment.

 
Lin,Nien-Tsung

Prof. Lin, a gracious teacher, is the guiders of our experiment on M13 phage display. She provides us a lot knowledge about M13 phage and DNA cloning.

     
Ching,Yung-Hao

Prof. Ching is one of the leader teachers of our team. She is a kind teacher who always encourages us, and she also gives us much advice and direction on safety concerns.

 
Woon,Peng-Yeong

Prof. Woon is one of the leader teachers for this year. He helps us the basic concept of iGEM and reminds us many iGEM rules or requests we nearly missed. He is a lively professor.

   
Experiment
 
Bobo, Regina, Denise, Eating
 
Contributions
 
  • Transformation ( Cas9 in pSB1C3 into DH5α)
  • Extract plasmid with pSB1C3 from DH5α.
  • Extract plasmid pSB1C3 with BBa_I13521、BBa_ K914003、BBa_ K1218011.
  • Use Enzyme digestion (PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.
  • Purification of RFP sequence from pSB1C3
  • Prepare Cm. plate、LB. plate.
  • Ligation of RFP and pBluescript SK(-).
  • Transformation clone RFP/pBluescript SK(-) to DH5α.
  • Extract plasmid clone RFP/pBluescript SK(-).
  • Experiment Design

    Ted and Bobo

    Design gRNA for AmpR.

    Biobricks Design

    Ted

    Design the gRNA for different resistance gene.

       
    Modeling
     
    House, Bobo, Chao-Di Chang(National Chiao Tung University)
     
    Contributions
     
  • Infectional efficiency ratio of M13KO7.
  • Release titer.(Releasing titer of phagemid which is E.coli infected by helper phage)
  • Special Thanks

    Modeling Advisor

    Chang,Chao-Di


    Thanks for Chang,Chao-Di strong support on our modeling.

       
    Human Practice
     
    Eating
     
    Contributions
     
  • Held the 2014 Synthetic Biology information meeting of local senior high school and Tzu Chi University as a chief convener.
  • Contact with National Chiao Tung University for cooperation.
  •    
    Wiki
     
    House
     
    Contributions
     
  • Construct the wiki page.
  • Coding,coding and coding the wiki page.
  • Study all about Synthetic Biology from wetlab to drylab.
  •  
    And last but not least
    Art Design

    Denise, Regina, Eating, House

    Design of poster and PPT.
    Design of team uniform.

     
    Photo & Video

    Sharon, Eating

    Take photo and video.
    Arrange all photos.

    Administeration

    Eating

    Apply for the allowances.
    Arrange all files.

     
       
    Video support
     
    Wang, Yiru and Chen, Jiawei
     
    we should be obliged to them for their assistance in this matter.
     

    Wang, Yi-ru

    Chen, Jia-wei

       
    Special Thanks
     
    Our partner : NCTU_Formosa
     
  • We are extremely appreciative of NCTU_Formosa that helped and support us.
  • The model1 courtesy of Chao-Di Chang.
  •    
    Sponsors
     
               
    General support
     
    Master program in Microbiology and Immunology
     
    Department of Medical Informatics
     
    Department of Molecular Biology and Human Genetics
       

     

    Acknowledgements

     

    We have taken efforts in this project. However, it would not have been possible without the kind support and help of many individuals and organizations. We would like to extend our sincere thanks to all of them.

    ^


        
    Team Members Project Parts Human Pratics Modeling Safety Notebook Attributions

    Lost the way? Use it to help you if you're lost.

    ^