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Team:Evry/Notebook/TransformationProto/08-05-2014
From 2014.igem.org
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| - | <u> <b> Tests for development of the electroporation's protocol </b> </u> <br> | + | <u> <b><big><FONT COLOR=#003333> Tests for development of the electroporation's protocol</font> </big></b> </u> <br> |
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<li>Re-launch of 50mL of Pseudovribrio in MB and M9 from pre-culture of 4th August. <br> | <li>Re-launch of 50mL of Pseudovribrio in MB and M9 from pre-culture of 4th August. <br> | ||
<li>Incubation at 30°C and waiting for the exponential growth phase.<br> | <li>Incubation at 30°C and waiting for the exponential growth phase.<br> | ||
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<li>Resuspend cells 1:100 of the initial volume with <u>cold sorbitol</u>, <u>cold MiliQ H2O</u> or <u>cold glycerol 10%</u> | <li>Resuspend cells 1:100 of the initial volume with <u>cold sorbitol</u>, <u>cold MiliQ H2O</u> or <u>cold glycerol 10%</u> | ||
<li>Stock cells in aliquots of 50µL at -80°C | <li>Stock cells in aliquots of 50µL at -80°C | ||
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Latest revision as of 15:08, 12 October 2014
Tests for development of the electroporation's protocol
> OD(600nm) in M9 = 0.15
> OD(600nm) in MB = 1.5
WORK IN ICE
