Team:Braunschweig

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        <title>E.&nbsp;cowli - Fighting Climate Change - iGEM 2014 Team Braunschweig</title>
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<img src="http://de.igem-braunschweig.de/wp-content/uploads/2014/06/cropped-iGEM-2014-Header-4-Logo.jpg">
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<h1>WELCOME TO THE "iGEM BRAUNSCHWEIG"-WIKI!</h1>
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Our Wiki is currently under construction.
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In the meantime visit our <a href="http://de.igem-braunschweig.de/" target="_blank"><b>homepage</b></a> or our <a href="http://www.facebook.com/iGEMBraunschweig" target="_blank">Facebook page</a> for further information on our team and our project.   
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            <ul>
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                <li>
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                    <a href="https://2014.igem.org/Team:Braunschweig">Home</a>
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                <li>
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                     <a href="https://2014.igem.org/Team:Braunschweig/Team">Team</a>
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                    <ul>
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                         <li><a href="#">Team</a></li>
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                        <li><a href="https://igem.org/Team.cgi?year=2014&team_name=Braunschweig">Official Team Profile</a>
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                        <li><a href="#">Collaboration</a></li>
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                        <li><a href="#">Gallery</a></li>
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<li><a href="https://2014.igem.org/Team:Braunschweig/Attributions">Attributions</a></li>
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                    <a href="https://2014.igem.org/Team:Braunschweig/Project">Project</a>
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                    <ul>
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                        <li><a href="#">Summary</a></li>
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                        <li><a href="#">Problem</a></li>
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                        <li><a href="#">Idea</a></li>
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                        <li><a href="#">Solution</a></li>
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<li><a href="#">Results</a></li>
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<li><a href="#">Potential</a></li>
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<li><a href="#">Engineering Principles</a></li>
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                </li>
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                <li class = "cat4">
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                    <a href="https://2014.igem.org/Team:Braunschweig/Parts">Parts</a>
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                        <li><a href="#">New Parts</a></li>
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                        <li><a href="#">Improved Parts</a></li>
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                        <li><a href="#">Part Collection</a></li>
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                    </ul>
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                <li class = "cat5">
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                    <a href="https://2014.igem.org/Team:Braunschweig/Modeling">Modeling</a>
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                    <ul>
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                        <li><a href="#">Modeling Approach</a></li>
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                        <li><a href="#">Mathematical Model</a></li>
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                        <li><a href="#">Code</a></li>
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                    </ul>
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                </li>
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<li class = "cat6">
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                    <a href="https://2014.igem.org/Team:Braunschweig/Notebook">Notebook</a>
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                        <li><a href="#">Protocols</a></li>
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                        <li><a href="#">MMO</a></li>
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                        <li><a href="#">His-Tag</a></li>
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                    <a href="https://2014.igem.org/Team:Braunschweig/Achievements">Achievements</a>
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                        <li><a href="#">Human Practices</a></li>
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                        <li><a href="#">Medal Requirements</a></li>
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                        <li><a href="#">Judging</a></li>
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                    <a href="https://2014.igem.org/Team:Braunschweig/Safety">Safety</a>
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                      <li><a href="#">Safety</a></li>
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<p> Please be sure to keep these links, your audience will want to find your: </p>
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<li><a href="https://2014.igem.org/Team:Braunschweig">Home</a> </li>
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            <li id="navbarhome"><a href="https://2014.igem.org/Team:Braunschweig">Home</a></li>
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<li><a href="https://2014.igem.org/Team:Braunschweig/Team">Team</a> </li>
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=Braunschweig">Official Team Profile</a> </li>
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<li><a href="https://2014.igem.org/Team:Braunschweig/Project">Project</a> </li>
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                  <li><a href="https://2014.igem.org/Team:Braunschweig/Team#profiles">Profiles</a></li>
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<li><a href="https://2014.igem.org/Team:Braunschweig/Parts">Parts</a> </li>
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                    <li><a href="https://2014.igem.org/Team:Braunschweig/Team-content#Collaborations">Collaboration</a></li>
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<li><a href="https://2014.igem.org/Team:Braunschweig/Modeling">Modeling</a> </li>
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                    <li><a href="https://2014.igem.org/Team:Braunschweig/Gallery">Gallery</a></li>
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<li><a href="https://2014.igem.org/Team:Braunschweig/Notebook">Notebook</a> </li>
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                    <li><a href="https://2014.igem.org/Team:Braunschweig/Team-content#Attributions">Attributions</a></li>
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<li><a href="https://2014.igem.org/Team:Braunschweig/Safety">Safety</a> </li>
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<li><a href="https://2014.igem.org/Team:Braunschweig/Attributions">Attributions</a> </li>
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                  <li><a target="_blank" href="https://igem.org/Team.cgi?year=2014&team_name=Braunschweig">Official Team Profile</a></li>  
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                </ul>
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            </li>
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            <li id='navbarproject'><a href="https://2014.igem.org/Team:Braunschweig/Project">Project</a>                   
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<ul id="nav-dd" style="margin-left:-105px">
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                    <li><a href="https://2014.igem.org/Team:Braunschweig/Project">Summary</a></li>
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                    <li><a href="https://2014.igem.org/Team:Braunschweig/Project-content#Problem">Problem</a></li>
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                    <li><a href="https://2014.igem.org/Team:Braunschweig/Project-content#Idea">Idea</a></li>
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                    <li><a href="https://2014.igem.org/Team:Braunschweig/Project-content#Approach">Approach</a></li>
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    <li><a href="https://2014.igem.org/Team:Braunschweig/Results">Results</a></li>
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    <li><a href="https://2014.igem.org/Team:Braunschweig/Project-content#Potential Impact">Impact</a></li>
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    <li><a href="https://2014.igem.org/Team:Braunschweig/Project-content#Engineering Principles">Engineering Principles</a></li>
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                </ul>
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            </li>
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            <li id="navbarparts"><a href="https://2014.igem.org/Team:Braunschweig/HP">Policy & Practices</a>                   
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                    <li><a href="https://2014.igem.org/Team:Braunschweig/HP#spreading">Spreading the Word</a></li>
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                    <li><a href="https://2014.igem.org/Team:Braunschweig/HP#teaching">Teaching</a></li>
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                    <li><a href="https://2014.igem.org/Team:Braunschweig/HP#opinions">Opinions</a></li>
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                </ul>
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            </li>
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            <li id="navbarmodeling"><a href="https://2014.igem.org/Team:Braunschweig/Modeling">Modeling</a>
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                <ul id="nav-dd" style="margin-left:-80px">
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                    <li><a href="https://2014.igem.org/Team:Braunschweig/Modeling-content#Modeling Approach">Modeling Approach</a></li>
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                    <li><a href="https://2014.igem.org/Team:Braunschweig/Modeling-content#MM">Mathematical Model</a></li>
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                    <li><a href="https://2014.igem.org/Team:Braunschweig/Modeling-content#Code">Code</a></li>
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</ul>
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            </li>
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            <li id="navbarnotebook"><a href="https://2014.igem.org/Team:Braunschweig/Notebook">Notebook</a>
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                <ul id="nav-dd" style="margin-left:-80px">
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                    <li><a href="https://2014.igem.org/Team:Braunschweig/Notebook-content#experiments">Experiments</a></li>
 +
                    <li><a href="https://2014.igem.org/Team:Braunschweig/Notebook-content#protocols">Protocols</a></li>
 +
                   
 +
                </ul>
 +
            </li>
 +
            <li id="navbarachievements"><a href="https://2014.igem.org/Team:Braunschweig/Achievements">Achievements</a>                 
 +
                <ul id="nav-dd"  style="margin-left:-130px">
 +
                    <li><a href="https://2014.igem.org/Team:Braunschweig/Achievements-content#parts">Parts</a></li>
 +
                    <li><a href="https://2014.igem.org/Team:Braunschweig/Achievements-content#mr">Medal Requirements</a></li>
 +
                    <li><a target="_blank" href="https://igem.org/2014_Judging_Form?id=1390">Judging</a></li>
 +
</ul>
 +
  </li>
 +
            <li id="navbarsafety"><a href="https://2014.igem.org/Team:Braunschweig/Safety">Safety</a>
 +
            </li>
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        </ul>
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</ul>
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                        </div><!--/.nav-collapse -->
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                    </div><!--/.container-fluid -->
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                </div><!--/.navbar -->
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            </div> <!-- /container -->
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        </div>
 +
<!-------- Slider -------------------->
 +
        <div>
 +
            <!-- Carousel -->
 +
            <div id="templatemo-carousel" class="carousel slide" data-ride="carousel">
 +
                <!-- Indicators -->
 +
                <ol class="carousel-indicators">
 +
                    <li data-target="#templatemo-carousel" data-slide-to="0" class="active"></li>
 +
                    <li data-target="#templatemo-carousel" data-slide-to="1"></li>
 +
                    <li data-target="#templatemo-carousel" data-slide-to="2"></li>
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                </ol>
 +
                <div class="carousel-inner">
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                    <div class="item active">
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                        <div class="container">
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                            <div class="carousel-caption">
 +
                                <h1><i style="font-size:50px">E.&nbsp;cowli</i></h1>
 +
                                <p style="font-size:47px">Fighting Climate Change at the Source</p>
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</td>
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                            </div>
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<td > </td>
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                        </div>
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<td width="45%">
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                    </div>
 +
                   
 +
                    <div class="item">
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                        <div class="container">
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                                    <div class="col-sm-6 col-md-6" style="margin-top:50px">
 +
                                    <h1><i style="font-size:38px">Kofi Annan</i></h1>
 +
<p style="font-size:30px">"On climate change, we often don't fully appreciate that it is a problem. We think it is a problem waiting to happen."</p>
 +
                                    </div>
 +
                                    <div class="col-sm-6 col-md-6" style="margin-top:50px">
 +
                                    <h1><i style="font-size:38px">Bill Gates</i></h1>
 +
                                    <p style="font-size:30px">"Climate change is a terrible problem, and it absolutely needs to be solved. It deserves to be a huge priority."</p>
 +
                                    </div>
 +
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 +
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                            <div class="container">
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                                <div class="carousel-caption">
 +
                                <h1><i style="font-size:45px">Bill Nye</i></h1>
 +
<p style="font-size:43px">"Climate change is happening, humans are causing it, and I think this is perhaps the most serious environmental issue facing us."</p>
 +
                                </div>
 +
                            </div>
 +
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                <a class="left carousel-control" href="#templatemo-carousel" data-slide="prev"><span class="glyphicon glyphicon-chevron-left"></span></a>
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        </div>
-
<p>There are a few wiki requirements teams must follow:</p>
 
-
<ul>
 
-
<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
 
-
<li>All pages must be created under the team’s name space.</li>
 
-
<li>As part of your documentation, keep the links from the menu to the left. </li>
 
-
<li>Do not use flash in wiki code. </li>
 
-
<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
 
-
</ul>
 
-
<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
 
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</td>
+
<!---------- CONTENT ------->
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</tr>
+
<div class="templatemo-welcome" id="templatemo-welcome">
 +
</div>
 +
<div class="container">
 +
<div class="pagesummary">
 +
<h1><i>E.&nbsp;cowli</i> - Fighting climate change at the source </h1>
 +
<p class="mr" style="text-align:justify;">Dairy and beef production are important to our daily lives and consumption needs. However, there are serious climate change issues regarding the continual expansion of these industries. The natural microbiota inside the cow’s rumen releases dangerous amounts of greenhouse gases as they help digest the animal's food. Therefore, finding ways to decrease the production of greenhouse gases of dairy cows and cattle is essential in fighting global warming. </p>
 +
<h3 align="center">In this year’s iGEM project, we fight global warming right at the source - the cow's rumen.</h3><br>
 +
<p class="mr" style="text-align:justify;">Using the powerful tools of synthetic biology we make it possible to reduce the methane levels through a genetically engineered bacterium named <i>E.&nbsp;cowli</i>, using the soluble form of the enzyme complex methane monooxygenase (sMMO) for hydroxylation of methane to methanol. We successfully expressed all the subunits in soluble form and, furthermore, were able to prove the activity of the whole enzyme complex. Moreover, the effectivity, feasibility as well as the safety of this project was evaluated using a mathematical model. <br><br>
 +
Besides the laboratory-related topics, we also initialized many more activities to spread the idea of synthetic biology and iGEM. On various occasions we had the chance to present ourselves and our project to the broad public. We taught synthetic biology not only in a lecture but also 1-on-1 in a hands-on internship with a pupil and we also used these events for evaluation of the people's opinions on synthetic biology and consumption of animal products. In many discussions with other teams we took the chance to debate iGEM-related topics such as ethics, safety and intellectual property.<br>
 +
Get started and find out more about us and our project!</p>
 +
                </div>
 +
            </div>
 +
        </div>
 +
       
 +
 
 +
<!------------------------->
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<tr> <td colspan="3"  height="15px"> </td></tr>
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<div class="container section">
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<tr><td bgColor="#be1b3c" colspan="3" height="1"> </tr>
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<div class="row">
 +
  <div class="col-md-4">
 +
  <div class="cbox">
 +
    <div>
 +
    <a href="https://2014.igem.org/Team:Braunschweig/Team">
 +
    <img class="box_img" src="https://static.igem.org/mediawiki/2014/3/36/TU-BS_Home_Team.jpg" alt="gallery1"/></a>
 +
    </div>
 +
    <div>
 +
    <span class="box_header">Team</span><br/>
 +
   
 +
    <p class="box_text">
 +
    We are this year's iGEM Team of the Technische Universität Braunschweig. Get to know us, our motivations and much more on our team profiles!
 +
    </p>
 +
   
 +
    </div>
 +
  </div>
 +
  </div> <!-- /.blog_post 1 TU-Day-->
 +
  <div class="col-md-4">
 +
  <div class="cbox">
 +
    <div>
 +
    <a href="https://2014.igem.org/Team:Braunschweig/HP">
 +
    <img class="box_img" src="https://static.igem.org/mediawiki/2014/1/11/TU-BS_Home_HP.jpg" alt="Oli and Maren"/>
 +
    </a>
 +
    </div>
 +
    <div>
 +
    <span class="box_header">Policy & Practices</span><br/>
 +
    <p class="box_text">
 +
iGEM is more than only working in a lab - it's spreading the word. Find out more about our events and actions on our Policy and Practices page.
 +
    </p>
 +
    </div>
 +
  </div>
 +
</div> <!-- /.blog_post 2-->
 +
<div class="col-md-4">
 +
  <div class="cbox">
 +
    <div><a href="https://2014.igem.org/Team:Braunschweig/Modeling">
 +
    <img class="box_img" src="https://static.igem.org/mediawiki/2014/3/3a/TU-BS_Home_Modeling.jpg" alt="gallery1"/>
 +
    </a>
 +
    </div>
 +
    <div>
 +
    <span class="box_header">Modeling</span><br/>
 +
    <p class="box_text">
 +
To evaluate the feasibilty of our project before has become reality we generated
 +
generated a mathematical model based on laboratory and literature data.
 +
</p>
 +
    </div>
 +
  </div>
 +
  </div> <!-- /.blog_post 3-->
 +
</div>
 +
</div>
-
<!--tips  -->
+
<div class="container section">
-
<tr><td colspan="3" > <h3> Tips </h3></td></tr>
+
<div class="cbox">
 +
<div class="row" style="
 +
    width: 1100px;">
 +
  <div class="col-md-5"><a href="https://2014.igem.org/Team:Braunschweig/Project"><img class="box_img" style="width: 400px; margin-left: 5px;"
 +
src="https://static.igem.org/mediawiki/2014/a/a9/TU-BS_Home_Project.jpg" alt="Kuh"/></a>
 +
  </div>
 +
  <div class="col-md-7" style="
 +
    width: 630px;">
 +
<h1>Project</h1></a>
 +
  <p class="box_text">The iGEM Team Braunschweig presents a novel approach towards the reduction of greenhouse gas emissions: We are going to equip the model organism <i>Escherichia coli</i> with the soluble methane monooxygenase (sMMO), an enzyme complex enabling methanotrophs to use the greenhouse gas methane as sole source of carbon and energy, hence creating our methane degrading <i>E.&nbsp;cowli</i>.
 +
  </p>
 +
  </div>
 +
  </div>
 +
</div>
 +
</div>
-
<tr>
 
-
<td width="45%" valign="top">
 
-
<p>We are currently working on providing teams with some easy to use design templates.
 
-
<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
 
-
<ul>
+
<div class="container section">
-
<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
+
<div class="row">
-
<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
+
<div class="col-md-4">
-
<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
+
  <div class="cbox">
-
<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
+
    <div>
-
<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
+
    <a href="https://2014.igem.org/Team:Braunschweig/Safety">
-
</ul>
+
    <img class="box_img" src="https://static.igem.org/mediawiki/2014/4/48/TU-BS_Home_Safety.JPG" alt="gallery1"/>
 +
    </a>
 +
    </div>
 +
    <div>
 +
    <span class="box_header">Safety</span><br/>
 +
    <p class="box_text">
 +
While designing our project, safety naturally was a very important issue for this year’s iGEM project. Issues in biosafety as well as gas health and safety were addressed.
 +
    </p>
 +
    </div>
 +
  </div>
 +
  </div> <!-- /.blog_post 1 TU-Day-->
 +
<div class="col-md-4">
 +
  <div class="cbox">
 +
    <div>
 +
    <a href="https://2014.igem.org/Team:Braunschweig/Notebook">
 +
    <img class="box_img" src="https://static.igem.org/mediawiki/2014/5/50/TU-BS_Home_Notebook.jpg" alt="Oli and Maren"/>
 +
    </a>
 +
    </div>
 +
    <div>
 +
    <span class="box_header">Notebook</span><br/>
 +
    <p class="box_text">
 +
Everyone who is interested in how we spent our iGEM summer, feel free to have a look at our Notebook, where we put together a documentation of our lab work.
 +
    </p>
 +
    </div>
 +
  </div>
 +
</div> <!-- /.blog_post 2-->
 +
<div class="col-md-4">
 +
  <div class="cbox">
 +
    <div class="img_circle">
 +
    <a href="https://2014.igem.org/Team:Braunschweig/Achievements">
 +
    <img class="box_img" src="https://static.igem.org/mediawiki/2014/1/13/TU-BS_Home_Achievements.jpg" alt="gallery1"/>
 +
    </a>
 +
    </div>
 +
    <div>
 +
    <span class="box_header">Achievements</span><br/>
 +
    <p class="box_text">
 +
By contributing BioBricks to the iGEM Registry of Standard Biological Parts so that other teams can use them in the future, we live up to the basic ideas of the iGEM competition.
-
<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
+
    </p>
-
</td>
+
    </div>
 +
  </div>
 +
  </div> <!-- /.blog_post 3-->
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</div>
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</div>
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<td> </td>
 
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<td width="45%">
 
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
        <div class="templatemo-footer" >
 +
            <div class="container">
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                <div class="row">
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                    <div class="text-center">
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                            <div class="height30"></div>
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                            <a class="btn" href="#">Back To Top</a>
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<div class="height30"></div>
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                        <div class="footer_bottom_content">Copyright © 2014 iGEM Team Braunschweig<br>
 +
<a href="mailto:igem.braunschweig@gmail.com">Contact us</a></div>
 +
                       
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                    </div>
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                </div>
 +
            </div>
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        </div>
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<ul>
+
    </body>
-
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
</html>
-
<li>Be clear about what you are doing and what you plan to do.</li>
+
-
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
-
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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Latest revision as of 20:24, 17 October 2014

E. cowli - Fighting Climate Change - iGEM 2014 Team Braunschweig

E. cowli - Fighting climate change at the source

Dairy and beef production are important to our daily lives and consumption needs. However, there are serious climate change issues regarding the continual expansion of these industries. The natural microbiota inside the cow’s rumen releases dangerous amounts of greenhouse gases as they help digest the animal's food. Therefore, finding ways to decrease the production of greenhouse gases of dairy cows and cattle is essential in fighting global warming.

In this year’s iGEM project, we fight global warming right at the source - the cow's rumen.


Using the powerful tools of synthetic biology we make it possible to reduce the methane levels through a genetically engineered bacterium named E. cowli, using the soluble form of the enzyme complex methane monooxygenase (sMMO) for hydroxylation of methane to methanol. We successfully expressed all the subunits in soluble form and, furthermore, were able to prove the activity of the whole enzyme complex. Moreover, the effectivity, feasibility as well as the safety of this project was evaluated using a mathematical model.

Besides the laboratory-related topics, we also initialized many more activities to spread the idea of synthetic biology and iGEM. On various occasions we had the chance to present ourselves and our project to the broad public. We taught synthetic biology not only in a lecture but also 1-on-1 in a hands-on internship with a pupil and we also used these events for evaluation of the people's opinions on synthetic biology and consumption of animal products. In many discussions with other teams we took the chance to debate iGEM-related topics such as ethics, safety and intellectual property.
Get started and find out more about us and our project!

Team

We are this year's iGEM Team of the Technische Universität Braunschweig. Get to know us, our motivations and much more on our team profiles!

Policy & Practices

iGEM is more than only working in a lab - it's spreading the word. Find out more about our events and actions on our Policy and Practices page.

Modeling

To evaluate the feasibilty of our project before has become reality we generated generated a mathematical model based on laboratory and literature data.

Kuh

Project

The iGEM Team Braunschweig presents a novel approach towards the reduction of greenhouse gas emissions: We are going to equip the model organism Escherichia coli with the soluble methane monooxygenase (sMMO), an enzyme complex enabling methanotrophs to use the greenhouse gas methane as sole source of carbon and energy, hence creating our methane degrading E. cowli.

Safety

While designing our project, safety naturally was a very important issue for this year’s iGEM project. Issues in biosafety as well as gas health and safety were addressed.

Notebook

Everyone who is interested in how we spent our iGEM summer, feel free to have a look at our Notebook, where we put together a documentation of our lab work.

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Achievements

By contributing BioBricks to the iGEM Registry of Standard Biological Parts so that other teams can use them in the future, we live up to the basic ideas of the iGEM competition.

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