Team:NCTU Formosa/Test

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===Researcher Safety===
 
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===團隊交流===
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1.Everyone must wear lab coat, trousers, gloves, surgery masks and shoes when carrying out experiments.
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2.Before the commencement of our project,our team instructors and advisers have guided us to go through every expiriment to make sure everybody is well trained and fit for this project.
 
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3.Everybody has to sterilize his/her hands and all the equipment platforms with alcohol solution(70%) before and after each expiriment.
 
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4.Emergency equipments are well prepared and all the researchers understand how to use these equipments and their exact position.
 
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5.It is not allowed that researcher carries experiment alone.There must be at least two members to conduct the experiment.
 
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6.Food and beverages are not allowed to appear in the lab.
 
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<div id="more1">111</div><div class="doo1">222</div>
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  {{:Team:NCTU Formosa/source/Test3}}
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<p>[[File:NCTU_Formosa_2014_HP_1.jpg|300px|thumb|right]]</p>
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{{:Team:NCTU Formosa/source/introo}}
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We invited Mingdao High school iGEM team to exchange ideas with us. We also held a pizza party to enhance more fun in this event.
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<div style="clear:both"></div>
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[[File:NCTU_Formosa_2014_HP_2.jpg|300px|thumb|right]]
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We asked Mingdao high school to give a brief introduction to their project－Odor Let It Die. It’s really an interesting and creative idea. Although they are just high school students, their English presenters clearly explained what problems they want to solve. In the discussion section, we gave them several feedback and suggestions to perfect their project.
 +
<div style="clear:both"></div>
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[[File:NCTU_Formosa_2014_HP_3.jpg|300px|thumb|right]]
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As for our presenting section, we successfully caught their eyes with our creative project－Operation Debug, which is to attract pests in the farm with PBAN.
 +
After our amazing presentation, we believed they not only learned more knowledge about synthetic biology but also impressed by the future development potential of this field.
 +
<div style="clear:both"></div>
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[[File:NCTU_Formosa_2014_HP_4.jpg|300px|thumb|right]]
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Because some high school students are pretty curious about our laboratory, so we guided them to pay a visit to lab at the end of this event. We introduce the equipment we usually use in the process of DNA synthesis.
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<div style="clear:both">
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====Video conferencing with Xiamen University ====
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<p></p>
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We had a video conferencing with the iGEM team of Xiamen University, China, in May. As both teams just started their own research projects at that time, the main topics of this video conferencing only consisted of a brief description or introduction of the protocols for each other’s project, how the research team was established and organized. According to this short conferencing, we knew the Xiamen team mainly consists of sophomores or juniors, whereas they were surprised to learn that almost 90% of our team members are freshmen.
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===Public safety===
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1.All collected lab waste are sterilized, packaged and executed.<br>
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<p id="para1">Me too!</p>
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<p>And me!</p>
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2.E.coli used in our lab is less competitive than wild type, because they are unable to form biofilms and to thrive in the intestine.<br>
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3.Liquid waste that contains E.coli is disinfected by adding peroxides and cleaners before it is disposed.<br>
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===Instruments in our lab===
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===戶外考察===
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'''1.Centrifuge'''<P>
 
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[[File:NCTU_Formosa_2014_Safty1.jpg|160px|thumb|center|]]
 
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A centrifuge is a piece of equipment, generally driven by an electric motor, that puts an object in rotation around a fixed axis, applying a force perpendicular to the axis. A centrifuge is also used to separate the components in a mixture. The centrifuge works using the sedimentation principle, where the centripetal acceleration causes denser substances to separate out along the radial direction (the bottom of the tube). By the same token lighter objects will tend to move to the top.<sup>1</sup> </p>
 
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'''2.incubator'''<p>
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====Dinning Together in Feng Wu====
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[[File:NCTU_Formosa_2014_Safty2.jpg|160px|thumb|center|]]
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The orbital shaker incubator is widely used in microbiology labs, cell biology labs, etc. It provides a stationary temperature to cultivate bacteria and cells. Some incubator also have functions to maintain the humility level.
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In our lab, the incubator is maintaining the temperature at 37 degrees Celsius, which is the optical temperature of E.coli growing.
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In this year, our team was authorized to work with an non-pathogenic bacterial strain, the Escherichia coli DH5. The strain ais regularly employed in research, industry and study. Biosafety Level 1 standard regulations were strictly followed while using these strains.</p>
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'''3.Autoclave'''<p>
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'''Experience Sharing of Capturing Bloodsucking Flies in the farm：'''<br>
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[[File:NCTU_Formosa_2014_Safty3.jpg|160px|thumb|center|]]
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　Dr. Tzeng told us the livestock farmers used to borrow the Insect light trap from them to capture the Bloodsucking Flies. To our surprise, he said the light trap can capture thousands of insects at just one night. Aditionally, they also had some cooperation program with livestock farmers. At that time, they used many different interesting approaches, like colors & light to test the behavior of Bloodsucking Flies. This experience sharing made us learn some basic methods of testing insects’ behavior.
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The autoclave is used for the safety issue. When sterilizing, the pressure and temperature inside reached high level, forming steam and superheat water. Cells will be killed and proteins will be destroyed or denatured. Thus, the safety is ensured.  
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<p></p>
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</p><p>In our lab, every equipment may contact bacteria will be sterilized  before and after use to guarantee the safety.</p>
+
'''The Art of Insect Rearing for Them：'''<br>
 +
　Dr. Tzeng told us insect rearing is not only a branch of knowledge, but a kind of art. For example, when malaria was a pandemic disease all over the world, some related research had difficulty in obvious progression, because Anopheles gambiae had not been reared successfully at that time. However, since a researcher from United Kingdom had success in rearing Anopheles gambiae in lab, the malaria disease had been completely researched, and the health life of human had been improved. This concept raised a great inspiration for how to do the research of our project.
 +
<p></p>
 +
'''Suggestion of Our Harmful Insects--Bactrocera dorsalis：'''<br>
 +
　Dr. Tzeng told us the bactrocera dorsalis is a serious problem for Taiwanese agriculture, which causes 17 billions dollors of loss every year. Our government had used many methods ( like：air drop many non-fertility bactrocera dorsalis to the farm ), trying to solve this problem, but finally failed. Dr. Tzeng suggested Bactrocera dorsalis as the target harmful insect of our project to solve the main problem of Taiwanese agricultural insects damage.
 +
<p></p>
 +
'''Discussion about Ecological Equilibrium Problem of Our Project：'''<br>
 +
　As for the ecological equilibrium problem of our project, Dr. Tzeng thought foundamentally we human farm in the area, having caused harm to nature. If you persist in the ecological equilibrium problem, then you have to ask yourself why you do farming and cause harm to the environment？Dr. Tzeng gave us a special view point, highlighting in the beginning, we human who do farming to survive have caused harm to the environment, so we shouldn’t inspect the ecological equilibrium problem of capturing insects.
 +
<p></p>
 +
'''Experience Sharing of Dengue Fever Prevention and Control：'''<br>
 +
　Dr. Tzeng shared the interesting experience of his presentation about Dengue Fever Prevention and Control at many town offices and also introduced there are two kinds of dengue fever vector mosquito, one is Aedes albopictus outdoors and the other is aedes aegypti indoors. We not only learned simple knowledge of dengue fever but also realized the feeling of helping people with professional knowledge.
-
'''4.Laminar flow'''<p>
 
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[[File:NCTU_Formosa_2014_Safty4.jpg|160px|thumb|center|]]
 
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A laminar flow is an enclosed, ventilated laboratory workspace for safely working with materials contaminated with (or potentially contaminated with) pathogens requiring a defined biosafety level. Several different types of BSC exist, differentiated by the degree of Laminar flow required.<sup>5</sup>
 
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We strictly rule that all researcher should conduct experiments inside the laminar flow.</p>
 
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'''5.Balance'''<p>
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====Visitation of their Department Building====
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[[File:NCTU_Formosa_2014_Safty5.jpg|160px|thumb|center|]]
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An analytical balance is a class of balance designed to measure small mass in the sub-milligram range. The measuring pan of an analytical balance (0.1 mg or better) is inside a transparent enclosure with doors so that dust does not collect and so any air currents in the room do not affect the balance's operation.<sup>3</sup>
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</p><p>In our lab, we have two models of balance, one is accurate to 0.1 g, another is accurate to 0.1 mg.
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</p>
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'''6.Water purify system'''<p>
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[[File:NCTU_Formosa_2014_Safty6.jpg|160px|thumb|center|]]
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In this system, Water is purified in a first step using unique Jetpore ion-exchange resin, synthetic activated carbon and UV lamp emitting at 185 and 254 nm to reach a resistivity of 18.2 MΩ.cm at 25 °C and a TOC value below 5 ppb, both monitored by advanced analytical techniques.<sup>4</sup>
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<td align=center>[[File:NCTU_Formosa_2014_HP_5.jpg|300px|thumb|center]]</td>
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The water purify system is used to produce ultrapure water, which is for experimental use in our lab.</p>
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<td align=center>[[File:NCTU_Formosa_2014_HP_6.jpg|300px|thumb|center]]</td>
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</table>
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</div>
 +
'''Explanation of the Real Function of Mosquito PBAN：'''<br>
 +
　In fact, the mosquito PBAN is activated during spreading wings when the mosquito emerges from its pupa. Initially, the mosquito wings are all living cells and when its PBAN regulates some hormones to make some cells apoptosis, just leaving few cells alive on its bone of wings, it finally forms transparent wings. This explanation about mosquito PBAN gave us a deeper realization about PBAN.
 +
<p></p>
-
'''7.Thermal cycler'''<P>
+
'''Take a Look at Their Mosquito Breeding Cage and Insect Light Trap ：'''<br>
-
[[File:NCTU_Formosa_2014_Safty7.jpg|160px|thumb|center|]]
+
　Dr. Tzeng let us take a look at Culex pipiens molestus (the underground house mosquito) breeding cage, and also introduced the behavior of many kinds of mosquito. This mosquito breeding cage inspired us many new ideas about our insects testing box. In fact, we mimicked the same concept of mosquito breeding cage to test our PBAN effect simply.
-
The thermal cycler (also known as a thermocycler, PCR machine or DNA amplifier) is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR). Thermal cyclers may also be used in laboratories to facilitate other temperature-sensitive reactions, including but not limited to restriction enzyme digestion or rapid diagnostics. The device has a thermal block with holes where tubes holding the reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps.<sup>2</sup> </p>
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<p></p>
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<div class=BOX>　　 
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<b>Reference</b>
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<table border=0  width=90% >
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<p><i>1.Centrifuge, Wikipedia,http://en.wikipedia.org/wiki/Centrifuge</i></p>
+
<td align=center>[[File:NCTU_Formosa_2014_HP_7.jpg|300px|thumb|center]]</td>
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<p><i>2.Thermal cycler ,Wikipedia,http://en.wikipedia.org/wiki/Thermal_cycler</i></p>
+
<td align=center>[[File:NCTU_Formosa_2014_HP_8.jpg|300px|thumb|center]]</td>
-
<p><i>3.Analytical balance, Wikipedia,http://en.wikipedia.org/wiki/Analytical_balance</i></p>
+
</table>
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<p><i>4.Milli-Q Direct, Millipore,http://www.millipore.com/catalogue/module/C85358</i></p>
+
</div>
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<p><i>5.Laminar flow (Biosafety cabinet), Wikipedia,http://en.wikipedia.org/wiki/Biosafety_cabinet</i></p>
+
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===2014 Safety Form===
+
<p></p>
 +
'''Further Discussion about our project：''' <br>
 +
[[File:NCTU_Formosa_2014_HP_10.jpg|300px|thumb|center]]
 +
　Dr. Tzeng have enthusiasm on discussing our project. To prevent any problem from happening, Dr. Tzeng and we inspected all the probability which may cause our project failed like PBAN degeadation in insects’ intestine ( whether we have to use little thuricide to cause some holes on insects’ intestine to make it absorb our PBAN easily ) and the restriction of our project ( we have to capture the first “female” insect in our device, and actually our project work. ). In the discussing process, we inspected many problems again, and also get many suggestion from Dr. Tzeng. We hoped we can overcome all the problems, and improve our project.
 +
<p></p>
-
<body>
+
'''Advantages Discussion of Our Device：'''<br>
 +
　Referring to our project, we use PBAN to make the harmful insects produce pheromone for us to help us attract many same kinds of insects. Dr. Tzeng was very interested in our project, and thought compared to the common use (Pheromone Trap & Pesticide ) in the market, our main idea have no dug resistance problem ( PBAN is insects’ physiologically natural substance ) and have no worry about the problem of running off our PBAN ( PBAN can be mass produced from our E.coli. ). It is a very user-friendly、eco-friendly and perfect project.
 +
<p></p>
 +
　In briefly conclusion, our team and Dr. Tzeng had deep discussion in this visitation. Not only experience sharing but also project inspection made us learn much and got many new ideas which let us realize how to make use of our project to achieve the best effect.
 +
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<a class="gallery slidea" href="#nogo" title="Fishes" tabindex="1"><span><img src=" https://static.igem.org/mediawiki/2014/0/08/NCTU_Formosa_2014_Safty8.jpg" alt="Fishes" title="Fishes" /><br />NCTU_Formosa_2014_Safty11.JPG<br />Photographs courtesy of morgueFile.com</span></a>
 
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<a class="gallery slidea" href="#nogo" title="Fishes" tabindex="1"><span><img src=" https://static.igem.org/mediawiki/2014/8/8e/NCTU_Formosa_2014_Safty9.jpg " alt="Fishes" title="Fishes" /><br />NCTU_Formosa_2014_Safty11.JPG<br />Photographs courtesy of morgueFile.com</span></a>
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===Futurework===
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<p></p>
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===Surveys===
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<p></p>
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<a class="gallery slidea" href="#nogo" title="Fishes" tabindex="1"><span><img src=" https://static.igem.org/mediawiki/2014/d/d8/NCTU_Formosa_2014_Safty10.JPG " alt="Fishes" title="Fishes" /><br />NCTU_Formosa_2014_Safty11.JPG<br />Photographs courtesy of morgueFile.com</span></a>
 
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{{:Team:NCTU Formosa/source/card-end}}
{{:Team:NCTU Formosa/source/card-end}}
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     <p>Cover image credit: <a href="http://www.dvq.co.nz/" target="_blank">DVQ</a></p>
     <p>Cover image credit: <a href="http://www.dvq.co.nz/" target="_blank">DVQ</a></p>
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Latest revision as of 15:05, 17 October 2014

Human Practice

Change the font size right here

Contents

團隊交流

This is an animated dialog which is useful for displaying information. The dialog window can be moved, resized and closed with the 'x' icon.

Open Dialog


111
222

This is an animated dialog which is useful for displaying information. The dialog window can be moved, resized and closed with the 'x' icon.

Open Dialog

NCTU Formosa 2014 HP 1.jpg

We invited Mingdao High school iGEM team to exchange ideas with us. We also held a pizza party to enhance more fun in this event.

NCTU Formosa 2014 HP 2.jpg

We asked Mingdao high school to give a brief introduction to their project－Odor Let It Die. It’s really an interesting and creative idea. Although they are just high school students, their English presenters clearly explained what problems they want to solve. In the discussion section, we gave them several feedback and suggestions to perfect their project.

NCTU Formosa 2014 HP 3.jpg

As for our presenting section, we successfully caught their eyes with our creative project－Operation Debug, which is to attract pests in the farm with PBAN. After our amazing presentation, we believed they not only learned more knowledge about synthetic biology but also impressed by the future development potential of this field.

NCTU Formosa 2014 HP 4.jpg

Because some high school students are pretty curious about our laboratory, so we guided them to pay a visit to lab at the end of this event. We introduce the equipment we usually use in the process of DNA synthesis.

Video conferencing with Xiamen University

We had a video conferencing with the iGEM team of Xiamen University, China, in May. As both teams just started their own research projects at that time, the main topics of this video conferencing only consisted of a brief description or introduction of the protocols for each other’s project, how the research team was established and organized. According to this short conferencing, we knew the Xiamen team mainly consists of sophomores or juniors, whereas they were surprised to learn that almost 90% of our team members are freshmen.


Every paragraph will be affected by the style.

Me too!

And me!

<style> body { 

   background: #ffffff url("NCTU_Formosa_2014_HP_1.jpg") no-repeat right top;
   margin-right: 200px;

} </style> </html>

戶外考察

Dinning Together in Feng Wu

Experience Sharing of Capturing Bloodsucking Flies in the farm：
　Dr. Tzeng told us the livestock farmers used to borrow the Insect light trap from them to capture the Bloodsucking Flies. To our surprise, he said the light trap can capture thousands of insects at just one night. Aditionally, they also had some cooperation program with livestock farmers. At that time, they used many different interesting approaches, like colors & light to test the behavior of Bloodsucking Flies. This experience sharing made us learn some basic methods of testing insects’ behavior.

The Art of Insect Rearing for Them：
　Dr. Tzeng told us insect rearing is not only a branch of knowledge, but a kind of art. For example, when malaria was a pandemic disease all over the world, some related research had difficulty in obvious progression, because Anopheles gambiae had not been reared successfully at that time. However, since a researcher from United Kingdom had success in rearing Anopheles gambiae in lab, the malaria disease had been completely researched, and the health life of human had been improved. This concept raised a great inspiration for how to do the research of our project.

Suggestion of Our Harmful Insects--Bactrocera dorsalis：
　Dr. Tzeng told us the bactrocera dorsalis is a serious problem for Taiwanese agriculture, which causes 17 billions dollors of loss every year. Our government had used many methods ( like：air drop many non-fertility bactrocera dorsalis to the farm ), trying to solve this problem, but finally failed. Dr. Tzeng suggested Bactrocera dorsalis as the target harmful insect of our project to solve the main problem of Taiwanese agricultural insects damage.

Discussion about Ecological Equilibrium Problem of Our Project：
　As for the ecological equilibrium problem of our project, Dr. Tzeng thought foundamentally we human farm in the area, having caused harm to nature. If you persist in the ecological equilibrium problem, then you have to ask yourself why you do farming and cause harm to the environment？Dr. Tzeng gave us a special view point, highlighting in the beginning, we human who do farming to survive have caused harm to the environment, so we shouldn’t inspect the ecological equilibrium problem of capturing insects.

Experience Sharing of Dengue Fever Prevention and Control：
　Dr. Tzeng shared the interesting experience of his presentation about Dengue Fever Prevention and Control at many town offices and also introduced there are two kinds of dengue fever vector mosquito, one is Aedes albopictus outdoors and the other is aedes aegypti indoors. We not only learned simple knowledge of dengue fever but also realized the feeling of helping people with professional knowledge.


Visitation of their Department Building

　　　　　　　　
NCTU Formosa 2014 HP 5.jpg
NCTU Formosa 2014 HP 6.jpg

Explanation of the Real Function of Mosquito PBAN：
　In fact, the mosquito PBAN is activated during spreading wings when the mosquito emerges from its pupa. Initially, the mosquito wings are all living cells and when its PBAN regulates some hormones to make some cells apoptosis, just leaving few cells alive on its bone of wings, it finally forms transparent wings. This explanation about mosquito PBAN gave us a deeper realization about PBAN.

Take a Look at Their Mosquito Breeding Cage and Insect Light Trap ：
　Dr. Tzeng let us take a look at Culex pipiens molestus (the underground house mosquito) breeding cage, and also introduced the behavior of many kinds of mosquito. This mosquito breeding cage inspired us many new ideas about our insects testing box. In fact, we mimicked the same concept of mosquito breeding cage to test our PBAN effect simply.

　　
NCTU Formosa 2014 HP 7.jpg
NCTU Formosa 2014 HP 8.jpg

Further Discussion about our project：

NCTU Formosa 2014 HP 10.jpg

　Dr. Tzeng have enthusiasm on discussing our project. To prevent any problem from happening, Dr. Tzeng and we inspected all the probability which may cause our project failed like PBAN degeadation in insects’ intestine ( whether we have to use little thuricide to cause some holes on insects’ intestine to make it absorb our PBAN easily ) and the restriction of our project ( we have to capture the first “female” insect in our device, and actually our project work. ). In the discussing process, we inspected many problems again, and also get many suggestion from Dr. Tzeng. We hoped we can overcome all the problems, and improve our project.

Advantages Discussion of Our Device：
　Referring to our project, we use PBAN to make the harmful insects produce pheromone for us to help us attract many same kinds of insects. Dr. Tzeng was very interested in our project, and thought compared to the common use (Pheromone Trap & Pesticide ) in the market, our main idea have no dug resistance problem ( PBAN is insects’ physiologically natural substance ) and have no worry about the problem of running off our PBAN ( PBAN can be mass produced from our E.coli. ). It is a very user-friendly、eco-friendly and perfect project.

　In briefly conclusion, our team and Dr. Tzeng had deep discussion in this visitation. Not only experience sharing but also project inspection made us learn much and got many new ideas which let us realize how to make use of our project to achieve the best effect.



Futurework


Surveys


Retrieved from "http://2014.igem.org/Team:NCTU_Formosa/Test"