Team:ETH Zurich/labblog/20140825meet
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- | *We have a Comsol simulation for | + | *We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length. |
[[File:ETH Zurich Comsol3.PNG|center|900px|thumb|Comsol simulation of diffusion experiments for 1mM and for 1 μM AHL initial concentration in sender wells. It explains why with 1mM all wells start glowing at the same time.]] | [[File:ETH Zurich Comsol3.PNG|center|900px|thumb|Comsol simulation of diffusion experiments for 1mM and for 1 μM AHL initial concentration in sender wells. It explains why with 1mM all wells start glowing at the same time.]] | ||
Revision as of 14:23, 1 September 2014
Week 14
Monday, August 25th
- We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths.
- We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length.
- We could fit degradation rates of external, internal AHL and GFP thanks to quorum sensing dynamic curves.
Fitting of quorum sensing dynamic parameters. Check the corresponding article for more details.