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Team:Gifu/Notebook
From 2014.igem.org
(Difference between revisions)
| Line 64: | Line 64: | ||
<h3>Restriction Enzyme Digestion</h3> | <h3>Restriction Enzyme Digestion</h3> | ||
| - | |||
| - | |||
<tr> | <tr> | ||
<th>sample</th><th>restriction enzymes</th> | <th>sample</th><th>restriction enzymes</th> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>His-RFP with stop codon</td><td>SpeI,EcoRI</td> | + | <td>His-RFP(with stop codon)</td><td>SpeI,EcoRI</td> |
</tr> | </tr> | ||
<td>5'-intron M</td><td>SpeI,EcoRI</td> | <td>5'-intron M</td><td>SpeI,EcoRI</td> | ||
| Line 77: | Line 75: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>His-RFP without stop codon</td><td>XbaI,EcoRI</td> | + | <td>His-RFP(without stop codon)</td><td>XbaI,EcoRI</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 99: | Line 97: | ||
<h3>Densitometry</h3> | <h3>Densitometry</h3> | ||
<table> | <table> | ||
| - | |||
<tr> | <tr> | ||
<th>sample</th><th>concentration(ng/µL)</th> | <th>sample</th><th>concentration(ng/µL)</th> | ||
| Line 180: | Line 177: | ||
</ol> | </ol> | ||
<h3>Restriction Enzyme Digestion</h3> | <h3>Restriction Enzyme Digestion</h3> | ||
| - | + | <table> | |
| + | <tr> | ||
| + | <th>sample</th><th>restriction enzymes</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>His-RFP(with stop codon)-DT-Cm</td><td>EcoRI,SpeI</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>5'intron M-DT-Cm</td><td>EcoRI,SpeI</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <h3>Densitometry</h3> | ||
| + | <table> | ||
| + | <tr> | ||
| + | <th>sample</th><th>concentration(ng/µL)</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td> | ||
<h3>Ligation</h3> | <h3>Ligation</h3> | ||
| + | <ol class="list"> | ||
| + | <li> | ||
<h3>Transformation</h3> | <h3>Transformation</h3> | ||
<p align="right"><a href="#calender">back to calender</a></p> | <p align="right"><a href="#calender">back to calender</a></p> | ||
Revision as of 04:27, 1 September 2014
Calender
Click on a date to see our research activities on that day.
| August | September | |||||||||||||||
| Mon | Tue | Wed | Thr | Fri | Sat | Sun | Mon | Tue | Wed | Thr | Fri | Sat | Sun | |||
| 1 | 2 | 3 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | |||||||
| 4 | 5 | 6 | 7 | 8 | 9 | 10 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | |||
| 11 | 12 | 13 | 14 | 15 | 16 | 17 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | |||
| 18 | 19 | 20 | 21 | 22 | 23 | 24 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | |||
| 25 | 26 | 27 | 28 | 29 | 30 | 31 | 29 | 30 | > | |||||||
August
August 11th
Restriction Enzyme Digestion
Ligation
- EcoRI-His-RFP(with stop codon)-SpeI + XbaI-DT-Cm-EcoRI
- EcoRI-5'intron M-SpeI + XbaI-DT-Cm-EcoRI
- EcoRI-Lac-3'intron-RBS-SpeI + XbaI-His-RFP(without stop codon)-5'intron-DT-Cm-EcoRI
- EcoRI-His-RFP(without stop codon)-SpeI + SpeI-Amp-EcoRI
Transformation
- His-RFP(with stop codon)-DT-Cm
- 5'intron M-DT-Cm
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm
- His-RFP(without stop codon)-Amp
Densitometry
| sample | concentration(ng/µL) |
|---|---|
| His-RFP(with stop codon) EcoRI-SpeI | 162.6 |
| 5'intron M EcoRI-SpeI | 45.4 |
| Lac-3'intron-RBS EcoRI-SpeI | 120.9 |
| His-RFP(without stop codon) EcoRI-SpeI | 160.0 |
| His-RFP(without stop codon)-5'intron-DT-Cm XbaI-EcoRI | 174.0 |
| Amp EcoRI-SpeI | 101.8 |
August 12th
Colony PCR
- His-RFP(with stop codon)-DT-Cm
- 5'intron M-DT-Cm
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm:[1]turning red
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm:[2]turning pink
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm:[3]turning white
- His-RFP(without stop codon)-Amp
Electrophoresis
- His-RFP(with stop codon)-DT
- His-RFP
- 5'intron M-DT
- 5'intron M
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT:[1]
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT:[2]
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT:[3]
- His-RFP(without stop codon)-5'intron-DT
- His-RFP(without stop codon)
Liquid Culture
- His-RFP(with stop codon)-DT
- 5'intron M-DT
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT:[1]
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT:[3]
- His-RFP(without stop codon)
August 13th
Miniprep
PCR
By use of KAPA HiFi DNA Polymerase- His-RFP(with stop codon)-DT-Cm
- 5'intron M-DT-Cm
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm:[1]turning red
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm:[3]turning white
Electrophoresis
- His-RFP(with stop codon)-DT-Cm
- 5'intron M-DT-Cm
- DNA Ladder Markers DNA Ladder One(Broad Range)
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm:[1]turning red
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm:[3]turning white
Restriction Enzyme Digestion
| sample | restriction enzymes |
|---|---|
| His-RFP(with stop codon)-DT-Cm | EcoRI,SpeI |
| 5'intron M-DT-Cm | EcoRI,SpeI |
