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| - | Previous page: [https://2014.igem.org/Team:ULB-Brussels/Project]
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| - | Next page: [https://2014.igem.org/Team:ULB-Brussels/Project/Results]
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| - | <!-- /** Design for https://2014.igem.org/Team:ULB-Brussels
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| - | Université Libre de Bruxelles **/ -->
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| | <!--requirements section --> | | <!--requirements section --> |
| - | | + | <tr style="background-color:rgb(245,245,245); box-shadow: 1px 1px 12px #555;"><td colspan="2"><p class="title"> An introduction to MightyColi</p></td></tr> |
| - | <tr style="background-color:rgb(245,245,245);"><td> | + | </table> |
| - | | + | </br> |
| | <table style="background-color:#ebebeb;" width="90%" align="center"> | | <table style="background-color:#ebebeb;" width="90%" align="center"> |
| | <tr style="background-color:rgb(245,245,245);"><td> | | <tr style="background-color:rgb(245,245,245);"><td> |
| - | <section style="text-align: center; margin: 25px"><p>
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| - | Some technical material used during our genetical experiments will be emphasized hereabouts. </p>
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| - | Different kinds of experimental methods have been applied in our Lab too. </p></section></table>
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| - | <section style="text-align: right; margin: -15px"><p>
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| - | ¨ </section>
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| - | <section style="text-align: right; margin: 50px">
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| - | <i>A selection of results obtained by our methods will be shown at the next section</i>.</p> </section>
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| - | <section style="text-align: right; margin: -35px"><p>
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| - | ¨ </section>
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| | <section style="text-align: justify; margin: 50px"> | | <section style="text-align: justify; margin: 50px"> |
| | + | A faire |
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| | + | </td> |
| | + | </tr> |
| | + | <tr><td><br/><br/></td></tr> |
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| - | <section style="text-align: center; margin: 30px"> | + | </table></th></tr> |
| - | <h3>Material</h3></section>
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| - | <section style="text-align: justify; margin: 0px">
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| - | A list of the Material is not yet accessible, but we hope this will be soon written. The usual equipment was:
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| - | gloves, glasses and coat (especially because of UV emission & Ethidium Bromide during electrophoresis).
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| - | There's more information in the page related with Safety.</p>
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| - | ________________________________________________________________________________________________________________
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| - | </p> </section>
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| - | <section style="text-align: center; margin: 30px">
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| - | <h3>Methods</h3></section>
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| - | <section style="text-align: justify; margin: 0px">
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| - | <p>First, birth and growing of bacteria populations (Centrifugation, Dilution).</p>
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| - | | + | |
| - | <p>Secondly, insertion of the biobricks and plasmids chosen with E. Coli (Electroporation method, PCR Amplification, Electrophoresis).
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| - | Just after this step, bacteria selection (in function of the quantities od oligopeptids or phoA+prolin) and inclusion of a toxin-antitoxin (TA: ccdB-ccdA) system in E. Coli.</p>
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| - | | + | |
| - | <p>Then, addiction of fluorescent proteins (GFP, RFP) and determination of the quantities and the principal properties of our bacteria
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| - | (including Emission Spectroscopy) and analize of their genetical sequences.
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| - | <p>Finally, conservation of bacteria populations producting the desired molecules or proteins in good quantities, at cold temperature in petri boxes and containers.</p></section>
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| - | </section>
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| - | <section style="text-align: right; margin: -20px"><p>
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| - | ¨ </section>
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| - | <tr><td><br/><br/></td></tr>
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| | </table> | | </table> |
| | </div> | | </div> |
$~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
\newcommand{\MyColi}{{\small Mighty\hspace{0.12cm}Coli}}
\newcommand{\Stabi}{\small Stabi}$
$\newcommand{\EColi}{\small E.coli}
\newcommand{\SCere}{\small S.cerevisae}\\[0cm]
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
\newcommand{\PI}{\small PI}$
$\newcommand{\Igo}{\Large\mathcal{I}}
\newcommand{\Tgo}{\Large\mathcal{T}}
\newcommand{\Ogo}{\Large\mathcal{O}}
~$
"
- Université Libre de Bruxelles -
