Team:ZJU-China/Project

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<p>   The assembly of genetic circuits is a big gap between theory and achievement in synthetic biology. Many well-known methods, from restriction endonuclease, 3A assembly to Gibson assembly, aim to overcome the difficulties. This year, ZJU-CHINA hope to build a gene-insertion system in bacterial chromosome, "Gene Socket". Different from the in vivo constructing methods mentioned before, Gene Socket can be used to constructing genetic circuits in chromosome directly as well as be combined with existing in vivo methods, which can make gene expression more accurate, stable and controllable.</p>
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<p>   The assembly of genetic circuits is a huge obstacle between designs and achievements in synthetic biology. Many well-known methods, from traditional restriction digestion & ligation, 3A assembly to Gibson assembly, aim to overcome the difficulties but unfortunately get respective defects. This year, ZJU-CHINA seeks to build a gene-insertion system in bacterial chromosome, "GeneSocket". Clearly different from the in vitro constructing methods mentioned before, GeneSocket, which can be easily combined with existing in vitro methods, makes gene expression more accurate, stable and controllable by assembling genetic elements in chromosome directly.</p>
<p>    Two core methods, lambda red recombination and recombinase-based bistable switch are applied to build our Gene Socket. Lambda red recombination is a widely used, efficient recombination system in prokaryotes. Recombinase-based bistable switch is relatively more stable and easier than transcription factor regulated bistable switch modules. Both the two are the best choices for achieving the characteristics of Gene Socket.</p>
<p>    Two core methods, lambda red recombination and recombinase-based bistable switch are applied to build our Gene Socket. Lambda red recombination is a widely used, efficient recombination system in prokaryotes. Recombinase-based bistable switch is relatively more stable and easier than transcription factor regulated bistable switch modules. Both the two are the best choices for achieving the characteristics of Gene Socket.</p>
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<p>    We hope that by using standardized Gene Socket, synthetic biologists can turn their theoretical design into reality faster and better, concentrating more on the designing and improvement of genetic circuits.</p>
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<p>    We hope that by using GeneSocket, synthetic biologists can turn their theoretical design into reality faster and better. We want to lead to the revolution in techniques of synthetic biology!</p>
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<h3>References </h3>
<h3>References </h3>

Revision as of 21:01, 29 August 2014


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Project Description

Content

The assembly of genetic circuits is a huge obstacle between designs and achievements in synthetic biology. Many well-known methods, from traditional restriction digestion & ligation, 3A assembly to Gibson assembly, aim to overcome the difficulties but unfortunately get respective defects. This year, ZJU-CHINA seeks to build a gene-insertion system in bacterial chromosome, "GeneSocket". Clearly different from the in vitro constructing methods mentioned before, GeneSocket, which can be easily combined with existing in vitro methods, makes gene expression more accurate, stable and controllable by assembling genetic elements in chromosome directly.

Two core methods, lambda red recombination and recombinase-based bistable switch are applied to build our Gene Socket. Lambda red recombination is a widely used, efficient recombination system in prokaryotes. Recombinase-based bistable switch is relatively more stable and easier than transcription factor regulated bistable switch modules. Both the two are the best choices for achieving the characteristics of Gene Socket.

We hope that by using GeneSocket, synthetic biologists can turn their theoretical design into reality faster and better. We want to lead to the revolution in techniques of synthetic biology!


References

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  2. Project Details
  3. Materials and Methods
  4. The Experiments
  5. Results
  6. Data analysis
  7. Conclusions

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