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Team:Evry/Notebook/Transformation/08-25-2014
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| - | <u> <b> Amplification of pRhokHI-2 </b> </u> <br> | + | <u> <b> Amplification of pRhokHI-2 in E.Coli Bl21 </b> </u> <br> |
We re-tried to transform E.Coli with pRhokHI-2 to amplify it <b>(LIEN PROTO)</b>. This time, after a liquid culture of only one hours cells were plated on LB 1X + Kan (1:1000) and let in incubator overnight. | We re-tried to transform E.Coli with pRhokHI-2 to amplify it <b>(LIEN PROTO)</b>. This time, after a liquid culture of only one hours cells were plated on LB 1X + Kan (1:1000) and let in incubator overnight. | ||
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Revision as of 14:30, 29 August 2014
Amplification of pRhokHI-2 in E.Coli Bl21
We re-tried to transform E.Coli with pRhokHI-2 to amplify it (LIEN PROTO). This time, after a liquid culture of only one hours cells were plated on LB 1X + Kan (1:1000) and let in incubator overnight.
Transformation of Pseudovribrio with pBBR1MCS
After made a MiniPrep purification of pBBR1MCS (LIEN PROTO), electrocompetent Pseudovibrio cells were tranformed with the plasmid by electroporation (LIEN PROTO), then they were incubated for three hours and showed on MB 1X+Cam (1:1000) plates. Plates are let in incubator overnight.
