Team:Gifu/Notebook
From 2014.igem.org
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<tr align="center"><td>4</td><td>5</td><td>6</td><td>7</td><td>8</td><td><font color="blue">9</td><td><font color="red">10</td> | <tr align="center"><td>4</td><td>5</td><td>6</td><td>7</td><td>8</td><td><font color="blue">9</td><td><font color="red">10</td> | ||
<td colspan="3"> </td><td><a href="#0908" style="color: black ;"><u>8</td><td><a href="#0909" style="color: black ;"><u>9</td><td><a href="#0910" style="color: black ;"><u>10</td><td><a href="#0911" style="color: black ;"><u>11</td><td><a href="#0912" style="color: black ;"><u>12</td><td><font color="blue"><a href="#0913" style="color: blue ;"><u>13</td><td><font color="blue"><a href="#0914" style="color: red ;"><u>14</td></tr> | <td colspan="3"> </td><td><a href="#0908" style="color: black ;"><u>8</td><td><a href="#0909" style="color: black ;"><u>9</td><td><a href="#0910" style="color: black ;"><u>10</td><td><a href="#0911" style="color: black ;"><u>11</td><td><a href="#0912" style="color: black ;"><u>12</td><td><font color="blue"><a href="#0913" style="color: blue ;"><u>13</td><td><font color="blue"><a href="#0914" style="color: red ;"><u>14</td></tr> | ||
- | <tr align="center"><td><a href="#0811" style="color: black ;"><u>11</u></td><td><a href="#0812" style="color: black ;"><u>12</u></a></td><td><a href="#0813" style="color: black ;"><u>13</u></a></td><td>14</u></a></td><td>15</u></a></td><td><font color="blue"><a href="#0816" style="color: blue;" ><u>16</u></td><td><font color="red"><u>17</u | + | <tr align="center"><td><a href="#0811" style="color: black ;"><u>11</u></td><td><a href="#0812" style="color: black ;"><u>12</u></a></td><td><a href="#0813" style="color: black ;"><u>13</u></a></td><td>14</u></a></td><td>15</u></a></td><td><font color="blue"><a href="#0816" style="color: blue;" ><u>16</u></td><td><font color="red"><u>17</u></td> |
<td colspan="3"> </td><td><a href="#0915" style="color: black ;"><u>15</td><td><a href="#0916" style="color: black ;"><u>16</td><td><a href="#0917" style="color: black ;"><u>17</td><td><a href="#0918" style="color: black ;"><u>18</td><td><a href="#0919" style="color: black ;"><u>19</td><td><font color="blue"><a href="#0920" style="color: blue;" ><u>20</td><td><font color="red"><a href="#0921" style="color: red;" ><u>21</td></tr> | <td colspan="3"> </td><td><a href="#0915" style="color: black ;"><u>15</td><td><a href="#0916" style="color: black ;"><u>16</td><td><a href="#0917" style="color: black ;"><u>17</td><td><a href="#0918" style="color: black ;"><u>18</td><td><a href="#0919" style="color: black ;"><u>19</td><td><font color="blue"><a href="#0920" style="color: blue;" ><u>20</td><td><font color="red"><a href="#0921" style="color: red;" ><u>21</td></tr> | ||
<tr align="center"><td><a href="#0818" style="color: black ;"><u>18</u></a></td><td><a href="#0819" style="color: black ;"><u>19</td><td><a href="#0820" style="color: black ;"><u>20</td><td><a href="#0821" style="color: black ;"><u>21</td><td><a href="#0822" style="color: black ;"><u>22</td><td><font color="blue"><a href="#0823" style="color: blue ;"><u>23</td><td><font color="red"><a href="#0824" style="color: red ;"><u>24</td> | <tr align="center"><td><a href="#0818" style="color: black ;"><u>18</u></a></td><td><a href="#0819" style="color: black ;"><u>19</td><td><a href="#0820" style="color: black ;"><u>20</td><td><a href="#0821" style="color: black ;"><u>21</td><td><a href="#0822" style="color: black ;"><u>22</td><td><font color="blue"><a href="#0823" style="color: blue ;"><u>23</td><td><font color="red"><a href="#0824" style="color: red ;"><u>24</td> |
Revision as of 07:13, 29 August 2014
Calender
Click on a date to see our research activities on that day.
August | September | |||||||||||||||
Mon | Tue | Wed | Thr | Fri | Sat | Sun | Mon | Tue | Wed | Thr | Fri | Sat | Sun | |||
1 | 2 | 3 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | |||||||
4 | 5 | 6 | 7 | 8 | 9 | 10 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | |||
11 | 12 | 13 | 14 | 15 | 16 | 17 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | |||
18 | 19 | 20 | 21 | 22 | 23 | 24 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | |||
25 | 26 | 27 | 28 | 29 | 30 | 31 | 29 | 30 | > |
August
August 11th
Restriction Enzyme Digestion
sample | restriction enzymes |
---|---|
His-RFP with stop codon | SpeI,EcoRI | 5'-intron M | SpeI,EcoRI |
Lac-3'intron-RBS | SpeI,EcoRI |
His-RFP without stop codon | XbaI,EcoRI |
RBS-Amp | SpeI,EcoRI |
Ligation
- EcoRI-His-RFP(with stop codon)-SpeI + XbaI-DT-Cm-EcoRI
- EcoRI-5'intron M-SpeI + XbaI-DT-Cm-EcoRI
- EcoRI-Lac-3'intron-RBS-SpeI + XbaI-His-RFP(without stop codon)-5'intron-DT-Cm-EcoRI
- EcoRI-His-RFP(without stop codon)-SpeI + SpeI-Amp-EcoRI
Transformation
- His-RFP(with stop codon)-DT-Cm
- 5'intron M-DT-Cm
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm
- His-RFP(without stop codon)-Amp
Densitometry
sample | concentration(ng/µL) |
---|---|
His-RFP(with stop codon) EcoRI-SpeI | 162.6 |
5'intron M EcoRI-SpeI | 45.4 |
Lac-3'intron-RBS EcoRI-SpeI | 120.9 |
His-RFP(without stop codon) EcoRI-SpeI | 160.0 |
His-RFP(without stop codon)-5'intron-DT-Cm XbaI-EcoRI | 174.0 |
Amp EcoRI-SpeI | 101.8 |