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Team:Gifu/Notebook
From 2014.igem.org
(Difference between revisions)
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<table> | <table> | ||
<h3>Electrophoresis</h3> | <h3>Electrophoresis</h3> | ||
| - | <h3>Shaking | + | <h3>Shaking Culture</h3> |
<p>To analysis sequence</p> | <p>To analysis sequence</p> | ||
<p align="right"><a href="#calender">back to calender</a></p> | <p align="right"><a href="#calender">back to calender</a></p> | ||
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<h2><a name="0818"><font color="black">August 18th</font></a></h2> | <h2><a name="0818"><font color="black">August 18th</font></a></h2> | ||
| - | <h3></h3> | + | <h3>Miniprep</h3> |
| + | <h3>Colony PCR</h3> | ||
| + | <h3>Densitometry</h3> | ||
| + | <h3>Ligation</h3> | ||
| + | <h3>Transformation</h3> | ||
| + | <h3>Analysis of DNA sequences</h3> | ||
<p align="right"><a href="#calender">back to calender</a></p> | <p align="right"><a href="#calender">back to calender</a></p> | ||
<h2><a name="0819"><font color="black">August 19th</font></a></h2> | <h2><a name="0819"><font color="black">August 19th</font></a></h2> | ||
| - | < | + | <h3>Colony PCR</h3> |
| + | <h3>Electrophoresis</h3> | ||
| + | <h3>Shaking Culture</h3> | ||
<p align="right"><a href="#calender">back to calender</a></p> | <p align="right"><a href="#calender">back to calender</a></p> | ||
<h2><a name="0820"><font color="black">August 20th</font></a></h2> | <h2><a name="0820"><font color="black">August 20th</font></a></h2> | ||
| - | <p></p> | + | <h3>Colony PCR</h3> |
| + | <p>Same as August 19th Colony PCR.</p> | ||
| + | <p align="right"><a href="#calender">back to calender</a></p> | ||
| + | |||
| + | <h2><a name="0821"><font color="black">August 21th</font></a></h2> | ||
| + | <h3>Reagent Preparation </h3> | ||
| + | <h3>Protein Extraction </h3> | ||
| + | <h3>SDS-PAGE</h3> | ||
| + | <p align="right"><a href="#calender">back to calender</a></p> | ||
| + | |||
| + | <h2><a name="0822"><font color="black">August 22th</font></a></h2> | ||
| + | <h3>Prorein Purification by use of Ni-NTA</h3> | ||
| + | <h3>SDS-PAGE</h3> | ||
| + | <p align="right"><a href="#calender">back to calender</a></p> | ||
| + | |||
| + | <h2><a name="0823"><font color="black">August 23th</font></a></h2> | ||
| + | <h3>Prorein Purification by use of Ni-NTA</h3> | ||
| + | <h3>SDS-PAGE</h3> | ||
| + | <p align="right"><a href="#calender">back to calender</a></p> | ||
| + | |||
| + | <h2><a name="0824"><font color="black">August 24th</font></a></h2> | ||
| + | <h3>Extraction of plasmid device</h3> | ||
| + | <p align="right"><a href="#calender">back to calender</a></p> | ||
| + | |||
| + | <h2><a name="0825"><font color="black">August 25th</font></a></h2> | ||
| + | <h3>Extraction of plasmid device</h3> | ||
| + | <h3>SDS-PAGE</h3> | ||
| + | <p align="right"><a href="#calender">back to calender</a></p> | ||
| + | |||
| + | <h2><a name="0826"><font color="black">August 26th</font></a></h2> | ||
| + | <h3>PCR</h3> | ||
| + | <h3>Restriction Enzyme Digestion</h3> | ||
| + | <h3>Densitometry</h3> | ||
| + | <h3>Ligation</h3> | ||
| + | <h3>Transformation</h3> | ||
| + | <p align="right"><a href="#calender">back to calender</a></p> | ||
| + | |||
| + | <h2><a name="0827"><font color="black">August 27th</font></a></h2> | ||
| + | <h3>Colony PCR</h3> | ||
| + | <h3>Shaking Culture</h3> | ||
| + | <p align="right"><a href="#calender">back to calender</a></p> | ||
| + | |||
| + | <h2><a name="0828"><font color="black">August 28th</font></a></h2> | ||
| + | <h3></h3> | ||
| + | <h3></h3> | ||
| + | <h3></h3> | ||
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Revision as of 07:02, 29 August 2014
Calender
Click on a date to see our research activities on that day.
| August | September | |||||||||||||||
| Mon | Tue | Wed | Thr | Fri | Sat | Sun | Mon | Tue | Wed | Thr | Fri | Sat | Sun | |||
| 1 | 2 | 3 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | |||||||
| 4 | 5 | 6 | 7 | 8 | 9 | 10 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | |||
| 11 | 12 | 13 | 14 | 15 | 16 | 17 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | |||
| 18 | 19 | 20 | 21 | 22 | 23 | 24 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | |||
| 25 | 26 | 27 | 28 | 29 | 30 | 31 | 29 | 30 | > | |||||||
August
August 11th
Restriction Enzyme Digestion
| sample | restriction enzymes |
|---|---|
| His-RFP with stop codon | SpeI,EcoRI | 5'-intron M | SpeI,EcoRI |
| Lac-3'intron-RBS | SpeI,EcoRI |
| His-RFP without stop codon | XbaI,EcoRI |
| RBS-Amp | SpeI,EcoRI |
Ligation
- EcoRI-His-RFP(with stop codon)-SpeI + XbaI-DT-Cm-EcoRI
- EcoRI-5'intron M-SpeI + XbaI-DT-Cm-EcoRI
- EcoRI-Lac-3'intron-RBS-SpeI + XbaI-His-RFP(without stop codon)-5'intron-DT-Cm-EcoRI
- EcoRI-His-RFP(without stop codon)-SpeI + SpeI-Amp-EcoRI
Transformation
- His-RFP(with stop codon)-DT-Cm
- 5'intron M-DT-Cm
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm
- His-RFP(without stop codon)-Amp
Densitometry
| sample | concentration(ng/µL) |
|---|---|
| His-RFP(with stop codon) EcoRI-SpeI | 162.6 |
| 5'intron M EcoRI-SpeI | 45.4 |
| Lac-3'intron-RBS EcoRI-SpeI | 120.9 |
| His-RFP(without stop codon) EcoRI-SpeI | 160.0 |
| His-RFP(without stop codon)-5'intron-DT-Cm XbaI-EcoRI | 174.0 |
| Amp EcoRI-SpeI | 101.8 |
