Team:Evry/Notebook/Transformation/08-26-2014

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<u> <b> Amplification of pRhokHI-2 </b> </u> <br>
 
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The transformation of E.Coli with pRhokHi-2 still didn't works. We still have to amplify it so we re-tried to the tranformation <b>(LIEN PROTO)</b>. <br>
 
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This time, after a liquid culture of only one hours cells were plated on LB 1X + Kan (1:1000), LB 1X + Cam (1:1000), and LB only and let in incubator overnight.
 
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<u> <b> Transformation of Pseudovribrio with pBBR1MCS </b> </u> <br>
<u> <b> Transformation of Pseudovribrio with pBBR1MCS </b> </u> <br>

Revision as of 21:40, 28 August 2014

Picture

Transformation of Pseudovribrio with pBBR1MCS
We saw no colony on our plates. We decided to test our two replicates of transformation on MB 1X plates with other concentrations of antibiotic. We made three plates with Cam 1:5000, and 1:10 000, then Pseudovibrio were showed on them and let overnight in incubator.

Aug 25

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