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Team:Evry/Notebook/Transformation/08-25-2014
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<u> <b> Transformation of Pseudovribrio with pBBR1MCS </b> </u> <br> | <u> <b> Transformation of Pseudovribrio with pBBR1MCS </b> </u> <br> | ||
| - | + | After made a MiniPrep purification of pBBR1MCS <b>(LIEN PROTO)</b>, electrocompetent Pseudovibrio cells were tranformed with the plasmid by electroporation <b>(LIEN PROTO)</b>, then they were incubated for three hours and showed on MB 1X+Cam (1:1000) plates. Plates are let in incubator overnight. | |
| - | + | ||
Revision as of 17:35, 28 August 2014
Amplification of pRhokHI-2
We re-tried to transform E.Coli with pRhokHI-2 to amplify it. This time, after a liquid culture of only one hours cells were plated on MB 1X + Kan (1:1000) and let in incubator overnight.
Transformation of Pseudovribrio with pBBR1MCS
After made a MiniPrep purification of pBBR1MCS (LIEN PROTO), electrocompetent Pseudovibrio cells were tranformed with the plasmid by electroporation (LIEN PROTO), then they were incubated for three hours and showed on MB 1X+Cam (1:1000) plates. Plates are let in incubator overnight.
