"
Team:Evry/Notebook/CellCharacterization/Transformation/08-23-2014
From 2014.igem.org
(Difference between revisions)
| Line 7: | Line 7: | ||
<p align="justify"> | <p align="justify"> | ||
| - | <u> <b> Amplification of plasmids pBBR1MCS and | + | <u> <b> Amplification of plasmids pBBR1MCS and pRhokHI-2 </b> </u> <br> |
| - | Two plasmids pBBR1MCS and | + | Two plasmids pBBR1MCS and pRhokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology) |
<br> | <br> | ||
<div align="center"> | <div align="center"> | ||
<img src="" alt="pBBR1MCS"/> | <img src="" alt="pBBR1MCS"/> | ||
| - | <img src="" alt=" | + | <img src="" alt="pRhokH1-2"/> |
</div> | </div> | ||
<br> | <br> | ||
Revision as of 13:09, 28 August 2014
Amplification of plasmids pBBR1MCS and pRhokHI-2
Two plasmids pBBR1MCS and pRhokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology)
Bowth were transfered into Bl21 electro-competent cells by electroporation (LIEN PROTO)(two replicate). After a liquid culture of three hours, cells were plated on MB 1X + Cam (1:1000) and let in incubator overnight. Aug 23
