"
Team:Gifu/Notebook
From 2014.igem.org
(Difference between revisions)
| Line 60: | Line 60: | ||
<h1 style="font-family:Comic Sans MS;font-size:x-large">August</h1> | <h1 style="font-family:Comic Sans MS;font-size:x-large">August</h1> | ||
| - | <h2 | + | <h2><a name="0811"> <font color="black">August 11th</font></a></h2> |
<h3>Restriction Digests</h3> | <h3>Restriction Digests</h3> | ||
| Line 128: | Line 128: | ||
<p align="right"><a href="#calender">back to calender</a></p> | <p align="right"><a href="#calender">back to calender</a></p> | ||
| - | <h2 | + | <h2><a name="0812"> <font color="black">August 12th</font></a></h2> |
<h3>Colony PCR</h3> | <h3>Colony PCR</h3> | ||
<h3>Electrophoresis</h3> | <h3>Electrophoresis</h3> | ||
Revision as of 10:26, 28 August 2014
Calender
Click on a date to see our research activities on that day.
| August | September | |||||||||||||||
| Mon | Tue | Wed | Thr | Fri | Sat | Sun | Mon | Tue | Wed | Thr | Fri | Sat | Sun | |||
| 1 | 2 | 3 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | |||||||
| 4 | 5 | 6 | 7 | 8 | 9 | 10 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | |||
| 11 | 12 | 13 | 14 | 15 | 16 | 17 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | |||
| 18 | 19 | 20 | 21 | 22 | 23 | 24 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | |||
| 25 | 26 | 27 | 28 | 29 | 30 | 31 | 29 | 30 | > | |||||||
August
August 11th
Restriction Digests
| object | restriction enzymes |
|---|---|
| His-RFP with stop codon | SpeI,EcoRI | 5'-intron M | SpeI,EcoRI |
| Lac-3'intron-RBS | SpeI,EcoRI |
| His-RFP without stop codon | XbaI,EcoRI |
| RBS-Amp | SpeI,EcoRI |
Ligation
- EcoRI-His-RFP(with stop codon)-SpeI + XbaI-DT-Cm-EcoRI
- EcoRI-5'intron M-SpeI + XbaI-DT-Cm-EcoRI
- EcoRI-Lac-3'intron-RBS-SpeI + XbaI-His-RFP(without stop codon)-5'intron-DT-Cm-EcoRI
- EcoRI-His-RFP(without stop codon)-SpeI + SpeI-Amp-EcoRI
Transformation
- His-RFP(with stop codon)-DT-Cm
- 5'intron M-DT-Cm
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm
- His-RFP(without stop codon)-Amp
Nano drop
| object | concentration(ng/µL) |
|---|---|
| His-RFP(with stop codon) EcoRI-SpeI | 162.6 |
| 5'intron M EcoRI-SpeI | 45.4 |
| Lac-3'intron-RBS EcoRI-SpeI | 120.9 |
| His-RFP(without stop codon) EcoRI-SpeI | 160.0 |
| His-RFP(without stop codon)-5'intron-DT-Cm XbaI-EcoRI | 174.0 |
| Amp EcoRI-SpeI | 101.8 |
| Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT |
August 12th
Colony PCR
Electrophoresis
Shaking culture
To analysis sequence
August 13th
sasisuseso
