Team:UNIK Copenhagen/Labbook

From 2014.igem.org

(Difference between revisions)
 
(2 intermediate revisions not shown)
Line 311: Line 311:
</div>
</div>
</div>
</div>
-
</html>
 
-
<html>
 
-
<style type="text/css">
 
-
#wrapper{
 
-
height: 1290px;
 
-
}
 
-
#indent_content{
 
-
margin: 30px;
 
-
padding: 0px;
 
-
width: 900px;
 
-
}
 
-
#all_content p{
 
-
font-family: "Times New Roman", sans-serif;
 
-
font-size:14px;
 
-
}
 
-
#all_content h1{
 
-
font-family: "Lucinda sans unicode", sans-serif;
 
-
}
 
-
#all_content{
 
-
margin: 30px;
 
-
padding: 0px;
 
-
border: 1px solid black;
 
-
width: 900px;
 
-
height: 570px;
 
-
background-color: #173B0B;
 
-
-moz-border-radius: 8px;
 
-
-webkit-border-radius: 8px;
 
-
}
 
-
.content{
 
-
height: 546px;
 
-
width: 790px;
 
-
background-image: none;
 
-
background-color: #FBF8EF;
 
-
background-size: 50%;
 
-
border: 1px solid grey;
 
-
padding: 10px;
 
-
margin: 0px;
 
-
text-align: justify;
 
-
}
 
-
.content img{
 
-
float: left;
 
-
margin: 10px;
 
-
}
 
-
.content table{
 
-
float: left;
 
-
border: none;
 
-
font-family: "Courier New";
 
-
background-color: #FBF8EF;
 
-
font-size: 12px;
 
-
}
 
-
.content table td{
 
-
padding:0px 8px;
 
-
}
 
-
.left_page, .right_page{
 
-
float: left;
 
-
width: 368px;
 
-
height: 526px;
 
-
margin: 13px;
 
-
}
 
-
#items{
 
-
float: left;
 
-
padding: 0px;
 
-
margin: 1px -41px 1px 41px;
 
-
}
 
-
#tabs{
 
-
margin: 0px;
 
-
padding: 0px;
 
-
}
 
-
#tabs li{
 
-
display: block;
 
-
list-style: none;
 
-
padding: 0px;
 
-
margin: 0px;
 
-
width: 46px;
 
-
height: 42px;
 
-
}
 
-
#tabs li a{
 
-
-moz-border-radius-topright: 10px;
 
-
-webkit-border-top-right-radius: 10px;
 
-
-moz-border-radius-bottomright: 10px;
 
-
-webkit-border-bottom-right-radius: 10px;
 
-
text-align: center;
 
-
display: block;
 
-
font-family: "Courier New";
 
-
font-size: 25px;
 
-
color: black;
 
-
background-color: #FBF8EF;
 
-
text-decoration: none;
 
-
border: 1px solid gray;
 
-
border-left: none;
 
-
padding: 9px 14px 28px 14px;
 
-
}
 
-
#tabs li a:hover{
 
-
background-color: #e3e3e5;
 
-
border: 1px solid #666666;
 
-
border-left: none;
 
-
}
 
-
#tabs li a.active{
 
-
background-color: #e3e3e5;
 
-
color: black;
 
-
border: 1px solid gray;
 
-
border-left: none;
 
-
}
 
-
#content2, #content3, #content4, #content5, #content6, #content7, #content8, #content9, #content10{
 
-
display: none;
 
-
padding: 10px;
 
-
}
 
-
#content4 p, #content7 p{
 
-
font-size: 12px;
 
-
}
 
-
#under{
 
-
width: 880px;
 
-
height: 400px;
 
-
float:left;
 
-
margin-left: -50px;
 
-
}
 
-
#under_page{
 
-
margin: 10px;
 
-
width: 200px;
 
-
float: left;
 
-
}
 
-
#under_page2{
 
-
margin: 10px;
 
-
width: 180px;
 
-
float: left;
 
-
}
 
-
#confocals{
 
-
height: 170px;
 
-
width: 340px;
 
-
float: left;
 
-
}
 
-
#confocals img{
 
-
margin: 5px;
 
-
}
 
-
#confocal{
 
-
overflow: hidden;
 
-
height: 160px;
 
-
width: 160px;
 
-
float: left;
 
-
}
 
-
#confocal img:hover{
 
-
margin-top: -165px;
 
-
}
 
-
#middle{
 
-
margin: 80px 50px 50px 55px;
 
-
}
 
-
</style>
 
-
 
-
 
-
 
-
<script type="text/javascript">
 
-
function ThisTab(new_tab, new_content) { 
 
-
    for(var i=1;i<11;i++){document.getElementById('content'+i).style.display = 'none';}
 
-
    document.getElementById(new_content).style.display = 'block';
 
-
 
-
    for(var i=1;i<11;i++){document.getElementById('tab'+i).className = '';}           
 
-
    document.getElementById(new_tab).className = 'active';
 
-
}
 
-
</script>
 
-
 
-
 
-
 
-
<div id="all_content">
 
-
 
-
 
-
<div id="tabs">
 
-
<div id="items">
 
-
<div id="content1" class="content">
 
-
<div class="left_page">
 
-
<p>First page.</p>
 
-
 
-
</div>
 
-
<div class="right_page">
 
-
</div>
 
-
</div>
 
-
 
-
<div id="content2" class="content">
 
-
<div class="left_page">
 
-
<p>Content2 (left)</p>
 
-
</div>
 
-
<div class="right_page">
 
-
<p>Content2 (right)</p>
 
-
</div>
 
-
</div>
 
-
 
-
<div id="content3" class="content">
 
-
<div class="left_page">
 
-
<p>Content3 (left)</p>
 
-
</div>
 
-
<div class="right_page">
 
-
<p>Content3 (right)</p>
 
-
</div>
 
-
</div>
 
-
 
-
<div id="content4" class="content">
 
-
<div class="left_page">
 
-
<p>Content4 (left)</p>
 
-
</div>
 
-
<div class="right_page">
 
-
<p>Content4 (right)</p>
 
-
</div>
 
-
</div>
 
-
 
-
<div id="content5" class="content">
 
-
<div class="left_page">
 
-
<p>Content5 (left)</p>
 
-
</div>
 
-
<div class="right_page">
 
-
<p>Content5 (right)</p>
 
-
</div>
 
-
</div>
 
-
 
-
<div id="content6" class="content">
 
-
<div class="left_page">
 
-
<p>Content6 (left)</p>
 
-
</div>
 
-
<div class="right_page">
 
-
<p>Content6 (right)</p>
 
-
</div>
 
-
</div>
 
-
 
-
<div id="content7" class="content">
 
-
<div class="left_page">
 
-
<p>Content7 (left)</p>
 
-
 
-
</div>
 
-
<div class="right_page">
 
-
<p>Content7 (right)</p>
 
-
</div>
 
-
</div>
 
-
 
-
<div id="content8" class="content">
 
-
<div class="left_page">
 
-
<p>Content8 (left)</p>
 
-
</div>
 
-
<div class="right_page">
 
-
<p>Content8 (right)</p>
 
-
</div>
 
-
</div>
 
-
 
-
<div id="content9" class="content">
 
-
<div class="left_page">
 
-
<p>Content9 (left)</p>
 
-
</div>
 
-
<div class="right_page">
 
-
<p>Content9 (right)</p>
 
-
</div>
 
-
</div>
 
-
 
-
<div id="content10" class="content">
 
-
<div class="left_page">
 
-
<p>Content10 (left)</p>
 
-
</div>
 
-
<div class="right_page">
 
-
<p>Content10 (right)</p>
 
-
</div>
 
-
</div>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
 
-
 
-
<div id="items">
 
-
<ul id="tabs">
 
-
    <li><a href="javascript:ThisTab('tab1', 'content1');" id="tab1" class="active">1</a></li> 
 
-
    <li><a href="javascript:ThisTab('tab2', 'content2');" id="tab2">2</a></li> 
 
-
    <li><a href="javascript:ThisTab('tab3', 'content3');" id="tab3">3</a></li>
 
-
    <li><a href="javascript:ThisTab('tab4', 'content4');" id="tab4">4</a></li>
 
-
    <li><a href="javascript:ThisTab('tab5', 'content5');" id="tab5">5</a></li>
 
-
    <li><a href="javascript:ThisTab('tab6', 'content6');" id="tab6">6</a></li>
 
-
    <li><a href="javascript:ThisTab('tab7', 'content7');" id="tab7">7</a></li> 
 
-
    <li><a href="javascript:ThisTab('tab8', 'content8');" id="tab8">8</a></li>
 
-
    <li><a href="javascript:ThisTab('tab9', 'content9');" id="tab9">9</a></li>
 
-
    <li><a href="javascript:ThisTab('tab10', 'content10');" id="tab10">10</a></li>
 
-
 
-
</ul>
 
-
</div>
 
-
</div>
 
-
</div>
 
-
</html>
 
-
 
-
<html>
 
<div id="footer" style="position:absolute;bottom:0;">
<div id="footer" style="position:absolute;bottom:0;">
<hr><br>
<hr><br>

Latest revision as of 19:55, 27 August 2014




17th of July - amplification of pETDuet-1 (Q)
Transformation of Ecloni with pETDuet-1 vector and plated the bacteria. They were afterwards stored in the fridge.

23rd of July (Q)
We made three liquid cultures each from a single colony on the plate.

24th of July(Q)
Minipreps of the pETDuet-1 vector. The minipreps was nanodroped. The measurements were:

Colony: ng/µl 260/280 260/230
1 30.3 1.96 2.34
2 63.3 1.82 1.89
3 32.1 1.82 1.68





On basis of the measurements, we picked sample 2 for sequencing. The sequences confirmed by sequencing.

20st of August (Q)
Ecloni was transformed with pUC57 containing sequences for either Estradiol light chain (EL) or Estradiol heavy chain (EH). The same was done for Progesterone light chain (PL) and heavy chain (PH). We also ran a PCR on pUC57, EL, EH, PL and PH.

Content2 (left)

Content2 (right)

Content3 (left)

Content3 (right)

Content4 (left)

Content4 (right)

Content5 (left)

Content5 (right)

Content6 (left)

Content6 (right)

Content7 (left)

Content7 (right)

Content8 (left)

Content8 (right)

Content9 (left)

Content9 (right)

Content10 (left)

Content10 (right)

Retrieved from "http://2014.igem.org/Team:UNIK_Copenhagen/Labbook"