Team:UNIK Copenhagen/Labbook

17^th of July - amplification of pETDuet-1 (Q)
Transformation of Ecloni with pETDuet-1 vector and plated the bacteria. They were afterwards stored in the fridge.

23^rd of July (Q)
We made three liquid cultures each from a single colony on the plate.

24^th of July(Q)
Minipreps of the pETDuet-1 vector. The minipreps was nanodroped. The measurements were:

On basis of the measurements, we picked sample 2 for sequencing. The sequences confirmed by sequencing.

20^st of August (Q)
Ecloni was transformed with pUC57 containing sequences for either Estradiol light chain (EL) or Estradiol heavy chain (EH). The same was done for Progesterone light chain (PL) and heavy chain (PH). We also ran a PCR on pUC57, EL, EH, PL and PH.

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