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Team:UChicago/Fluctuation Analysis
From 2014.igem.org
(Difference between revisions)
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| Line 8: | Line 8: | ||
margin: auto; | margin: auto; | ||
width: 60%; | width: 60%; | ||
| + | } | ||
| + | |||
| + | table { | ||
| + | width: 100%; | ||
} | } | ||
| Line 18: | Line 22: | ||
<div id="maincontent"> | <div id="maincontent"> | ||
| - | <input type="text" id="text" style="width: 150px;"> | + | <input type="text" id="text" style="width: 150px;"/> |
<br/> | <br/> | ||
| - | <input type="button" value="Click Me" id="button"> | + | <input type="button" value="Click Me" id="button"/> |
<br/> | <br/> | ||
<h2>Small FALCOR-like program for mutational analysis</h2> | <h2>Small FALCOR-like program for mutational analysis</h2> | ||
| + | <input type="checkbox" id="debugb"><label for="debugb">Debug mode</label></input> | ||
| + | <button type="button">Add sample data</button> | ||
<div id="inputs"> | <div id="inputs"> | ||
<h4>Mutants</h4> | <h4>Mutants</h4> | ||
| - | <textarea id="mutin" | + | <textarea id="mutin" placeholder="Paste data here"></textarea> |
<h4>Viable Cells</h4> | <h4>Viable Cells</h4> | ||
| - | <textarea id="tcellsin" | + | <textarea id="tcellsin" placeholder="Paste data here"></textarea> |
| + | <button id="calcb" type="button">Calculate</button> | ||
</div> | </div> | ||
<div id="results"> | <div id="results"> | ||
| Line 54: | Line 61: | ||
text.val(text.val() + ' after clicking'); | text.val(text.val() + ' after clicking'); | ||
}); | }); | ||
| + | }); | ||
| + | $(document).ready(function() { | ||
| + | $('#calcb').click(function() { | ||
| + | var mutcounts = $.map($('#mutin').val().split(), parseInt); | ||
| + | if (mutcounts.some(isNaN)) { | ||
| + | alert('invalid value of mutant amount given'); | ||
| + | return; | ||
| + | } | ||
| + | var viablescounts = $.map($('#tcellsin').val().split(),parseInt); | ||
| + | if (viablescounts.some(isNaN)) { | ||
| + | alert('invalid value of number of viable cells'); | ||
| + | return; | ||
| + | } | ||
| + | if (mutcounts.length < 1 || viablescounts.length < 1) { | ||
| + | alert('must have at least one data member in each input'); | ||
| + | return; | ||
| + | } | ||
| + | if (mutcounts.length != viablescounts.length) { | ||
| + | alert('must have same number of data items in all inputs'); | ||
| + | return; | ||
| + | } | ||
| + | var tmutplates = 0; | ||
| + | for (var i = 0; i < mutcounts.length; i++) { | ||
| + | if (mutcounts[i] > 0) tmutplates++; | ||
| + | } | ||
| + | var hitspercell = Math.log(mutcounts.length / tmutplates); | ||
| + | var mutrate = hitspercell / viablescounts[0]; | ||
| + | $('#mutrate').html(mutrate.toString()); | ||
| + | }); | ||
}); | }); | ||
</script> | </script> | ||
Latest revision as of 02:52, 26 August 2014
Small FALCOR-like program for mutational analysis
Mutants
Viable Cells
Results
| Mean Mutational Events | 95% CI | Mutation Rate | 95% CI |
|---|---|---|---|
| 1.4 | .1 | 10-3 | 10-4 |
