Team:Evry/Notebook/CellCharacterization/Antibiotic test/08-17-2014

From 2014.igem.org

(Difference between revisions)
(Created page with "<html> <div class="cd-timeline-block"> </html> {{:Team:Evry/Notebook/CellCharacterization/Antibiotic/TopImage}} <html> <div class="cd-timeline-content"> <p align="justify">...")
Line 32: Line 32:
There was a problem with the stock of Kanamycine so we made a new one at 25mg/mL.
There was a problem with the stock of Kanamycine so we made a new one at 25mg/mL.
-
For the erythrompycine, we learn that E. Coli is not really sensitive to this antibiotic and that it's better to use it  with a dilution at 1:100
+
For the erythrompycine, we learn that E. Coli is not really sensitive to this antibiotic and that it's better to use it  with a dilution at 1:100.<br>
<u>Survivability tests - Results</u> <br>
<u>Survivability tests - Results</u> <br>

Revision as of 17:47, 25 August 2014

Picture

Tests of antibiotics' stocks - Results

Plates LB Agar only LB+Cam LB+Kan LB+Amp LB+Erm LB+Tet
E.coli X 0 X 0 X 0
There was a problem with the stock of Kanamycine so we made a new one at 25mg/mL. For the erythrompycine, we learn that E. Coli is not really sensitive to this antibiotic and that it's better to use it with a dilution at 1:100.
Survivability tests - Results
liquid cultures/Plates MB M9
MB ++++ ++
M9 ++ +
Any liquid culture can be plated and will grow up. Of course, it works better on MB medium, wich is a rich medium than on M9.

Aug 17

Retrieved from "http://2014.igem.org/Team:Evry/Notebook/CellCharacterization/Antibiotic_test/08-17-2014"