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Team:Evry/Notebook/Sensing/PCBs/08-14-2014
From 2014.igem.org
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<u>Survival test on E.coli BL21:</u> | <u>Survival test on E.coli BL21:</u> | ||
| - | This test allowed to know if E.coli can survive at different concentrations of 2 hydroxy-3',4'dichlorobiphenyl | + | <br/>This test allowed to know if E.coli can survive at different concentrations of 2 hydroxy-3',4'dichlorobiphenyl |
<ol> | <ol> | ||
<li>Culturing of 200µL BL21 in 4mL of LB | <li>Culturing of 200µL BL21 in 4mL of LB | ||
Revision as of 16:32, 25 August 2014
Sensor construction HbpR/PhbpC/HbpC:
- Culturing DH5a (pHYB130 plasmid) in 3mL LB + 3µL Amp
- Incubation at 37°C, 200rpm
Survival test on E.coli BL21:
This test allowed to know if E.coli can survive at different concentrations of 2 hydroxy-3',4'dichlorobiphenyl
- Culturing of 200µL BL21 in 4mL of LB
- Incubation overnight at 37°C, 200rpm
