Team:Evry/Notebook/Sensing/PCBs/07-27-2014
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<u>Sensor construction bphR1/bphR2:</u> | <u>Sensor construction bphR1/bphR2:</u> | ||
<ol> | <ol> | ||
- | <li>Culturing a colony bphR1 promoter <a href="http://parts.igem.org/Part:BBa_K1155001">(BBa_K1155001)</a>, received from Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam) | + | <li>Culturing a colony bphR1 promoter <a href="http://parts.igem.org/Part:BBa_K1155001">(BBa_K1155001)</a>, received from Paris-Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam) |
<li>Incubation at 37°C overnight, 200rpm | <li>Incubation at 37°C overnight, 200rpm | ||
</ol> | </ol> |
Revision as of 16:28, 25 August 2014
Sensor construction bphR1/bphR2:
- Culturing a colony bphR1 promoter (BBa_K1155001), received from Paris-Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam)
- Incubation at 37°C overnight, 200rpm