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Team:Evry/Notebook/Sensing/PCBs/08-15-2014
From 2014.igem.org
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| - | <u | + | <u>Sensor construction HbpR/PhbpC/HbpC:</u> |
Purification of pHYB130 plasmid with NucleoSpin plasmid protocol => we obtained 91,8ng/µL | Purification of pHYB130 plasmid with NucleoSpin plasmid protocol => we obtained 91,8ng/µL | ||
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| - | <u | + | <u>Survival test on E.coli BL21:</u> |
A lab stock of 2 hydroxy-3',4'dichlorobiphenyl at 10(-2)mol/L was made by dissolving 10mg in DMSO | A lab stock of 2 hydroxy-3',4'dichlorobiphenyl at 10(-2)mol/L was made by dissolving 10mg in DMSO | ||
<ol> | <ol> | ||
Revision as of 10:25, 25 August 2014
Sensor construction HbpR/PhbpC/HbpC:
Purification of pHYB130 plasmid with NucleoSpin plasmid protocol => we obtained 91,8ng/µL
Survival test on E.coli BL21:
A lab stock of 2 hydroxy-3',4'dichlorobiphenyl at 10(-2)mol/L was made by dissolving 10mg in DMSO
- Serial dilution of compound was made from 10(-2) mol/L to 10(-8) mol/L
- 300µL of E.coli BL21 was added in each eppendorf tube.
We had 2 control tubes:- A negative control: 2,7mL of M9 medium + 300µL of compound
- A positive control: 2,7mL of M9 medium + 300µL of E.coli BL21
- Incubation of tubes at 37°C overnight, 200rpm Aug 15
