Team:Evry/Notebook/Sensing/PCBs/08-07-2014

From 2014.igem.org

(Difference between revisions)
(Created page with " <html> <div class="cd-timeline-block"> </html> {{:Team:Evry/Notebook/Biology/TopImage}} <html> <div class="cd-timeline-content"> <p align="justify"> <u>Part Extract...")
Line 1: Line 1:
-
 
<html>
<html>
   <div class="cd-timeline-block">
   <div class="cd-timeline-block">
Line 8: Line 7:
       <p align="justify">
       <p align="justify">
-
<u>Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:</u>
+
<p>
-
<br/> Culturing of 1 colony in 5mL of LB + 5µL Amp and incubation at 37°C, 200rpm
+
<u><h4>Sensor construction HbpR/PhbpC:</h4></u>
 +
<ol>
 +
<li> Culturing of a colony DH5a with HbpR-PhbpC sequence in 5mL of LB + 5µL Amp
 +
<li> Incubation at 37°C, 200rpm
 +
</ol>
 +
 
</p>
</p>

Revision as of 10:08, 25 August 2014

Picture

Sensor construction HbpR/PhbpC:

  1. Culturing of a colony DH5a with HbpR-PhbpC sequence in 5mL of LB + 5µL Amp
  2. Incubation at 37°C, 200rpm

Aug 7