Team:Evry/Interlab Study/08-22-2014

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<br/> A PCR was performed on 8 colonies for BBa_J23115 (K823012) following the protocol Table 3 and 4.
<br/> A PCR was performed on 8 colonies for BBa_J23115 (K823012) following the protocol Table 3 and 4.
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<br/> To verify PCR products, 10 µl was loaded on a 1% agarose gel with 2 µl of loading dye 6X.
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    <center>1% agarose gel of PCR products of the eight selected colonies</center>
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<br/> We expected to obtain a band between 300 and 400 bp.
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Revision as of 14:08, 23 August 2014

Picture


Transformation plate observation:
- BBa_J23101: nothing grown
- BBa_J23115 (K823012): 100-150 isolated colonies

A PCR was performed on 8 colonies for BBa_J23115 (K823012) following the protocol Table 3 and 4.
To verify PCR products, 10 µl was loaded on a 1% agarose gel with 2 µl of loading dye 6X.

IMAGE
Symbol
1% agarose gel of PCR products of the eight selected colonies

We expected to obtain a band between 300 and 400 bp.

Aug 22

Retrieved from "http://2014.igem.org/Team:Evry/Interlab_Study/08-22-2014"