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Team:Gifu/Notebook
From 2014.igem.org
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<td colspan="3"> </td><td><a href="#0922" style="color: black ;"><u>22</td><td><a href="#0923" style="color: black ;"><u>23</td><td><a href="#0924" style="color: black ;"><u>24</td><td><a href="#0925" style="color: black ;"><u>25</td><td><a href="#0926" style="color: black ;"><u>26</td><td><font color="blue"><a href="#0927" style="color: blue;" ><u>27</td><td><font color="red"><a href="#0928" style="color: red;" ><u>28</td></tr> | <td colspan="3"> </td><td><a href="#0922" style="color: black ;"><u>22</td><td><a href="#0923" style="color: black ;"><u>23</td><td><a href="#0924" style="color: black ;"><u>24</td><td><a href="#0925" style="color: black ;"><u>25</td><td><a href="#0926" style="color: black ;"><u>26</td><td><font color="blue"><a href="#0927" style="color: blue;" ><u>27</td><td><font color="red"><a href="#0928" style="color: red;" ><u>28</td></tr> | ||
<tr align="center"><td><a href="#0825" style="color: black ;"><u>25</td><td><a href="#0826" style="color: black ;"><u>26</td><td><a href="#0827" style="color: black ;"><u>27</td><td><a href="#0828" style="color: black ;"><u>28</td><td><a href="#0829" style="color: black ;"><u>29</td><td><font color="blue"><a href="#0830" style="color: blue ;"><u>30</td><td><font color="red"><a href="#08" style="color: red ;"><u>31</td> | <tr align="center"><td><a href="#0825" style="color: black ;"><u>25</td><td><a href="#0826" style="color: black ;"><u>26</td><td><a href="#0827" style="color: black ;"><u>27</td><td><a href="#0828" style="color: black ;"><u>28</td><td><a href="#0829" style="color: black ;"><u>29</td><td><font color="blue"><a href="#0830" style="color: blue ;"><u>30</td><td><font color="red"><a href="#08" style="color: red ;"><u>31</td> | ||
| - | <td colspan="3"> </td><td><a href="#0929" style="color: black ;"><u>29</td><td><a href="#0930" style="color: black ;"><u>30</td><td></td><td></td>><td></td><td></td></tr> | + | <td colspan="3"> </td><td><a href="#0929" style="color: black ;"><u>29</td><td><a href="#0930" style="color: black ;"><u>30</td><td></td><td></td>><td></td><td></td><td></td></tr> |
</tbody></table> | </tbody></table> | ||
Revision as of 03:10, 23 August 2014
Calender
Click on a date to see our research activities on that day.
| August | September | |||||||||||||||
| Mon | Tue | Wed | Thr | Fri | Sat | Sun | Mon | Tue | Wed | Thr | Fri | Sat | Sun | |||
| 1 | 2 | 3 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | |||||||
| 4 | 5 | 6 | 7 | 8 | 9 | 10 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | |||
| 11 | 12 | 13 | 14 | 15 | 16 | 17 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | |||
| 18 | 19 | 20 | 21 | 22 | 23 | 24 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | |||
| 25 | 26 | 27 | 28 | 29 | 30 | 31 | 29 | 30 | > | |||||||
August
August 11th
Restriction Digests
| object | restriction enzymes |
|---|---|
| His-RFP with stop codon | SpeI,EcoRI | 5'-intron M | SpeI,EcoRI |
| Lac-3'intron-RBS | SpeI,EcoRI |
| His-RFP without stop codon | XbaI,EcoRI |
| RBS-Amp | SpeI,EcoRI |
Ligation
- EcoRI-His-RFP(with stop codon)-SpeI + XbaI-DT-Cm-EcoRI
- EcoRI-5'intron M-SpeI + XbaI-DT-Cm-EcoRI
- EcoRI-Lac-3'intron-RBS-SpeI + XbaI-His-RFP(without stop codon)-5'intron-DT-Cm-EcoRI
- EcoRI-His-RFP(without stop codon)-SpeI + SpeI-Amp-EcoRI
Transformation
- His-RFP(with stop codon)-DT-Cm
- 5'intron M-DT-Cm
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm
- His-RFP(without stop codon)-Amp
Nano drop
| object | concentration(ng/µL) |
|---|---|
| His-RFP(with stop codon) EcoRI-SpeI | 162.6 |
| 5'intron M EcoRI-SpeI | 45.4 |
| Lac-3'intron-RBS EcoRI-SpeI | 120.9 |
| His-RFP(without stop codon) EcoRI-SpeI | 160.0 |
| His-RFP(without stop codon)-5'intron-DT-Cm XbaI-EcoRI | 174.0 |
| Amp EcoRI-SpeI | 101.8 |
| Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT |
August 12th
Colony PCR
Electrophoresis
Shaking culture
To analysis sequence
August 13th
sasisuseso
