"
Team:Gifu/Notebook
From 2014.igem.org
(Difference between revisions)
| Line 111: | Line 111: | ||
<li>His-RFP(without stop codon)-Amp</li> | <li>His-RFP(without stop codon)-Amp</li> | ||
</ul> | </ul> | ||
| - | <h3>Nano drop | + | <h3>Nano drop</h3> |
<p align="right"><a href="#calender">back to calender</a></p> | <p align="right"><a href="#calender">back to calender</a></p> | ||
Revision as of 04:33, 22 August 2014
Calender
Click on a date to see our research activities on that day.
| August | September | |||||||||||||||
| Mon | Tue | Wed | Thr | Fri | Sat | Sun | Mon | Tue | Wed | Thr | Fri | Sat | Sun | |||
| 1 | 2 | 3 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | |||||||
| 4 | 5 | 6 | 7 | 8 | 9 | 10 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | |||
| 11 | 12 | 13 | 14 | 15 | 16 | 17 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | |||
| 18 | 19 | 20 | 21 | 22 | 23 | 24 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | |||
| 25 | 26 | 27 | 28 | 29 | 30 | 31 | 29 | 30 | ||||||||
August
August 11th
Restriction Digests
| object | restriction enzymes |
|---|---|
| His-RFP with stop codon | SpeI,EcoRI | 5'-intron M | SpeI,EcoRI |
| Lac-3'intron-RBS | SpeI,EcoRI |
| His-RFP without stop codon | XbaI,EcoRI |
| RBS-Amp | SpeI,EcoRI |
Ligation
- EcoRI-His-RFP(with stop codon)-SpeI + XbaI-DT-Cm-EcoRI
- EcoRI-5'intron M-SpeI + XbaI-DT-Cm-EcoRI
- EcoRI-Lac-3'intron-RBS-SpeI + XbaI-His-RFP(without stop codon)-5'intron-DT-Cm-EcoRI
- EcoRI-His-RFP(without stop codon)-SpeI + SpeI-Amp-EcoRI
Transformation
- His-RFP(with stop codon)-DT-Cm
- 5'intron M-DT-Cm
- Lac-3'intron-RBS-His-RFP(without stop codon)-5'intron-DT-Cm
- His-RFP(without stop codon)-Amp
Nano drop
August 12
sasisuseso
August 13
sasisuseso
