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Team:Gifu/Notebook
From 2014.igem.org
(Difference between revisions)
| Line 99: | Line 99: | ||
<h3>Ligation</h3> | <h3>Ligation</h3> | ||
<ul> | <ul> | ||
| - | <li> | + | <li>/EcoRI-His-RFP(with stop codon)-SpeI/ + /XbaI-DT-Cm-EcoRI/</li> |
| - | <li> | + | <li>/EcoRI-5'intron M-SpeI/ + /XbaI-DT-Cm-EcoRI/</li> |
| - | <li> | + | <li>/EcoRI-Lac-3'intron-RBS-SpeI/ + /XbaI-His-RFP(without stop codon)-5'intron-DT-Cm-EcoRI/</li> |
| - | <li> | + | <li>/EcoRI-His-RFP(without stop codon)-SpeI/ + /SpeI-Amp-EcoRI/</li> |
</ul> | </ul> | ||
<h3>Transformation</h3> | <h3>Transformation</h3> | ||
Revision as of 03:10, 22 August 2014
Calender
Click on a date to see our research activities on that day.
| August | September | |||||||||||||||
| Mon | Tue | Wed | Thr | Fri | Sat | Sun | Mon | Tue | Wed | Thr | Fri | Sat | Sun | |||
| 1 | 2 | 3 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | |||||||
| 4 | 5 | 6 | 7 | 8 | 9 | 10 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | |||
| 11 | 12 | 13 | 14 | 15 | 16 | 17 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | |||
| 18 | 19 | 20 | 21 | 22 | 23 | 24 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | |||
| 25 | 26 | 27 | 28 | 29 | 30 | 31 | 29 | 30 | ||||||||
August
August 11th
Restriction Digests
| object | restriction enzymes |
|---|---|
| His-RFP with stop codon | SpeI,EcoRI | 5'-intron M | SpeI,EcoRI |
| Lac-3'intron-RBS | SpeI,EcoRI |
| His-RFP without stop codon | XbaI,EcoRI |
| RBS-Amp | SpeI,EcoRI |
Ligation
- /EcoRI-His-RFP(with stop codon)-SpeI/ + /XbaI-DT-Cm-EcoRI/
- /EcoRI-5'intron M-SpeI/ + /XbaI-DT-Cm-EcoRI/
- /EcoRI-Lac-3'intron-RBS-SpeI/ + /XbaI-His-RFP(without stop codon)-5'intron-DT-Cm-EcoRI/
- /EcoRI-His-RFP(without stop codon)-SpeI/ + /SpeI-Amp-EcoRI/
Transformation
August 12
sasisuseso
August 13
sasisuseso
