Team:UT-Dallas/Notebook/8-19

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       <br> RBS-Carb Gel was good.  
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      <br> Updating the wiki notebook from the slowest computer in the building because currently we can't log into any other computer. Rishika is gel-ing the ctx-s.
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Revision as of 18:35, 19 August 2014

Tuesday, August 19, 2014

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Today is a short day. Yesterday, we picked 2 colonies from each failed clone plates and inoculate them; today, we minipreped and test digested them.

We are sending the gRNA from the third batch and some other reporter vectors for sequencing.

Yesterday, we digested more RBS-Carb; today, we ran it on gel and gel purified it. The concentration at the end was good.

Our clones on the plates transformed yesterday were good: many colonies on the plates and none on the negative.

Orientation! School is starting next week! (25/8) >.<

Today's tasks:
  • Miniprep ctxA, ctxB.
  • Test digest and ran gel for ctxA, ctxB.
  • Inoculate 2 colonies from each plate transformed yesterday (second batch, reporter vectors on Carb).
  • Prepared for sequencing: diluted to the correct volume and concentration.
  • Purified RBS (digested overnight yesterday).
  • Transformed of overnight ligation yesterday (third batch, reporter vectors with promoters under Chlora).


RBS-Carb Gel was good.


Updating the wiki notebook from the slowest computer in the building because currently we can't log into any other computer. Rishika is gel-ing the ctx-s.

Retrieved from "http://2014.igem.org/Team:UT-Dallas/Notebook/8-19"