Team:UT-Dallas/Notebook/8-19

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<h1 class="firstHeading" align="center">Wednesday, August 13, 2014</h1>
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<h1 class="firstHeading" align="center">Tuesday, August 19, 2014</h1>
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   Today is a short day. We are test digesting Tra's minipreped first batch of reporter vectors on Carb backbone in the weekend. Two clones failed so we picked 2 colonies from each failed clone plates and inoculate them. We will miniprep and test digesting them again tomorrow. We threw out the miniprep products and glycerol stocks for the failed one. We got some confusion with the gel because Tra loaded multiple wells with the same sample. (Sorry for the confusion! - Tra)</p>
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   Today is a short day. Yesterday, we picked 2 colonies from each failed clone plates and inoculate them; today, we minipreped and test digested them. </p>
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We are sending the gRNA from the third batch and some other reporter vectors for sequencing.</p>
We are sending the gRNA from the third batch and some other reporter vectors for sequencing.</p>
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We also digest RBS-Carb to get more Carb back bone for later use.</p>
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Yesterday, we digested more RBS-Carb; today, we ran it on gel and gel purified it. The concentration at the end was good.</p>
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We started the third batch last week with some gel digest testing of gRNA. Only one (tcpF) failed. We are building our reporter vectors today from PCR products.</p>
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Our clones on the plates transformed yesterday were good: many colonies on the plates and none on the negative.</p>
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<strike>We also had a lively discussion about bugs. Tra's new apartment has bed bugs. Ewww!</strike>
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<strike>Orientation! School is starting next week! (25/8) >.<</strike>
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Revision as of 18:27, 19 August 2014

Tuesday, August 19, 2014

Click here to edit the page

Today is a short day. Yesterday, we picked 2 colonies from each failed clone plates and inoculate them; today, we minipreped and test digested them.

We are sending the gRNA from the third batch and some other reporter vectors for sequencing.

Yesterday, we digested more RBS-Carb; today, we ran it on gel and gel purified it. The concentration at the end was good.

Our clones on the plates transformed yesterday were good: many colonies on the plates and none on the negative.

Orientation! School is starting next week! (25/8) >.<

Today's tasks:
  • Miniprep ctxA, ctxB.
  • Test digest and ran gel for ctxA, ctxB.
  • Inoculate 2 colonies from each plate transformed yesterday (second batch, reporter vectors on Carb).
  • Prepared for sequencing: diluted to the correct volume and concentration.
  • Purified RBS (digested overnight yesterday).
  • Transformed of overnight ligation yesterday (third batch, reporter vectors with promoters under Chlora).


Rishika sitting on the table.


Description of image from today

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