Revision as of 18:21, 19 August 2014

Wednesday, August 13, 2014

Today is a short day. We are test digesting Tra's minipreped first batch of reporter vectors on Carb backbone in the weekend. Two clones failed so we picked 2 colonies from each failed clone plates and inoculate them. We will miniprep and test digesting them again tomorrow. We threw out the miniprep products and glycerol stocks for the failed one. We got some confusion with the gel because Tra loaded multiple wells with the same sample. (Sorry for the confusion! - Tra)

We are sending the gRNA from the third batch and some other reporter vectors for sequencing.

We also digest RBS-Carb to get more Carb back bone for later use.

We started the third batch last week with some gel digest testing of gRNA. Only one (tcpF) failed. We are building our reporter vectors today from PCR products.

~~We also had a lively discussion about bugs. Tra's new apartment has bed bugs. Ewww!~~

Today's tasks:
Miniprep ctxA, ctxB. Test digest and ran gel for ctxA, ctxB. Inoculate 2 colonies from each plate transformed yesterday (second batch, reporter vectors on Carb). Prepared for sequencing: diluted to the correct volume and concentration. Purified RBS (digested overnight yesterday). Transformed of overnight ligation yesterday (third batch, reporter vectors with promoters under Chlora).

Rishika sitting on the table.

Description of image from today

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 <p class="tab">
-   Today is a long day. We are test digesting Tra's minipreped first batch of reporter vectors on Carb backbone in the weekend. Two clones failed so we picked 2 colonies from each failed clone plates and inoculate them. We will miniprep and test digesting them again tomorrow. We threw out the miniprep products and glycerol stocks for the failed one. We got some confusion with the gel because Tra loaded multiple wells with the same sample. (Sorry for the confusion! - Tra)</p>
+   Today is a short day. We are test digesting Tra's minipreped first batch of reporter vectors on Carb backbone in the weekend. Two clones failed so we picked 2 colonies from each failed clone plates and inoculate them. We will miniprep and test digesting them again tomorrow. We threw out the miniprep products and glycerol stocks for the failed one. We got some confusion with the gel because Tra loaded multiple wells with the same sample. (Sorry for the confusion! - Tra)</p>
 <p class="tab">
-We are digesting second batch of reporter vectors, ligate on Carb and transform them. Hopefully tomorrow there will be colonies on our plates. We will pick the colonies, inoculate. The day after tomorrow we will glycerol stock them, miniprep and test digest our product. Hopefully we will have good product to complete parts of our BioBricks, (and we won't have to redo everything again) and we will keep them for later experiments.</p>
+We are sending the gRNA from the third batch and some other reporter vectors for sequencing.</p>
 <p class="tab">
 We also digest RBS-Carb to get more Carb back bone for later use.</p>