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Team:Evry/Notebook/Sensing/PCBs/week5
From 2014.igem.org
(Difference between revisions)
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<u>bphR1 promoter:</u> | <u>bphR1 promoter:</u> | ||
<ol> | <ol> | ||
| - | <li> Culturing bphR1 promoter <a href="http://parts.igem.org/Part:BBa_K1155001">BBa_K1155001</a>, received from Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam) | + | <li> Culturing 1 colony bphR1 promoter <a href="http://parts.igem.org/Part:BBa_K1155001">BBa_K1155001</a>, received from Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam) |
<li> Incubation at 37°C overnight | <li> Incubation at 37°C overnight | ||
</ol> | </ol> | ||
| Line 20: | Line 20: | ||
<u>bphR1 promoter:</u> | <u>bphR1 promoter:</u> | ||
<ol> | <ol> | ||
| - | <li> Plate | + | <li> Plate 20µL on LB-agar-Cam (25 mL LB-Agar + 25µL Cam) |
| - | <li> Incubation at 37°C overnight and glycerol | + | <li> Incubation of plate at 37°C overnight and the rest was stored in glycerol 50% at -80°C |
| + | |||
</p> | </p> | ||
Latest revision as of 11:58, 19 August 2014
Week 5
Sensor construction bphR1/bphR2
07/27/14:
bphR1 promoter:- Culturing 1 colony bphR1 promoter BBa_K1155001, received from Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam)
- Incubation at 37°C overnight
07/28/14:
bphR1 promoter:- Plate 20µL on LB-agar-Cam (25 mL LB-Agar + 25µL Cam)
- Incubation of plate at 37°C overnight and the rest was stored in glycerol 50% at -80°C
