Team:Evry/Notebook/Sensing/PCBs/week5

From 2014.igem.org

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<div class="week">
<div class="week">
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   <h4>Week 5
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   <h4>Week 5</h4>
   <div class="Sensing/PCBs">
   <div class="Sensing/PCBs">
<p>
<p>
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<h2> Sensor construction bphR1/bphR2 </h2>
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<u><h3> Sensor construction bphR1/bphR2 </h3></u>
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'''bphR1 promoter:'''
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<h4>07/27/14:</h4>
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<br/> Culturing bphR1 promoter (BBa_K1155001), received from Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam)
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<u>bphR1 promoter:</u>
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<br/> Incubation at 37°C overnight
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<ol>
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<br/> Plate of 1 colony on LB-agar-Cam (25 ml Lb-Agar + 25µL Cam)
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<li> Culturing 1 colony bphR1 promoter <a href="http://parts.igem.org/Part:BBa_K1155001">BBa_K1155001</a>, received from Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam)
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<br/> Incubation at 37°C overnight and glycerol stock
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<li> Incubation at 37°C overnight
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</ol>
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<h4>07/28/14:</h4>
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<u>bphR1 promoter:</u>
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<ol>
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<li> Plate 20µL on LB-agar-Cam (25 mL LB-Agar + 25µL Cam)
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<li> Incubation of plate at 37°C overnight and the rest was stored in glycerol 50% at -80°C
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</p>
</p>

Latest revision as of 11:58, 19 August 2014

Week 5

Sensor construction bphR1/bphR2

07/27/14:

bphR1 promoter:
  1. Culturing 1 colony bphR1 promoter BBa_K1155001, received from Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam)
  2. Incubation at 37°C overnight

07/28/14:

bphR1 promoter:
  1. Plate 20µL on LB-agar-Cam (25 mL LB-Agar + 25µL Cam)
  2. Incubation of plate at 37°C overnight and the rest was stored in glycerol 50% at -80°C

Retrieved from "http://2014.igem.org/Team:Evry/Notebook/Sensing/PCBs/week5"