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Team:Paris Bettencourt/Notebook/Eliminate Smell
From 2014.igem.org
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| - | Goal: PCR agaA gBlock with oPB.001 and oPB.005 </p> | + | '''Goal: PCR agaA gBlock with oPB.001 and oPB.005''' </p> |
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| - | <p>Procedure:</p> | + | <p>'''Procedure:'''</p> |
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<p>We are going to PCR using the DreamTaq polymerase protocol the agaA gBlock with the forward primer oPB.001 and the reverse primer oPB.005. These two oligos include the BioBrick prefix and suffix. The aim is to add the prefix and suffix to the agaA sequence and amplify it.</p> | <p>We are going to PCR using the DreamTaq polymerase protocol the agaA gBlock with the forward primer oPB.001 and the reverse primer oPB.005. These two oligos include the BioBrick prefix and suffix. The aim is to add the prefix and suffix to the agaA sequence and amplify it.</p> | ||
Revision as of 11:00, 19 August 2014
Paris Bettencourt 2014
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Eliminate Smell
Notebook
June
June 23rd
'''Goal: PCR agaA gBlock with oPB.001 and oPB.005'''
'''Procedure:'''
We are going to PCR using the DreamTaq polymerase protocol the agaA gBlock with the forward primer oPB.001 and the reverse primer oPB.005. These two oligos include the BioBrick prefix and suffix. The aim is to add the prefix and suffix to the agaA sequence and amplify it.
We will set the parameters in the machine and run the reaction overnight.
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July
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August
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September
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October
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