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Team:Evry/Notebook/Sensing/PCBs/week5
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<h2> Sensor construction bphR1/bphR2 </h2> | <h2> Sensor construction bphR1/bphR2 </h2> | ||
'''bphR1 promoter:''' | '''bphR1 promoter:''' | ||
| - | Culturing bphR1 promoter (BBa_K1155001), received from Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam) | + | <br/> Culturing bphR1 promoter (BBa_K1155001), received from Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam) |
| - | Incubation at 37°C overnight | + | <br/> Incubation at 37°C overnight |
| - | Plate of 1 colony on LB-agar-Cam (25 ml Lb-Agar + 25µL Cam) | + | <br/> Plate of 1 colony on LB-agar-Cam (25 ml Lb-Agar + 25µL Cam) |
| - | Incubation at 37°C overnight and glycerol stock | + | <br/> Incubation at 37°C overnight and glycerol stock |
</p> | </p> | ||
Revision as of 15:56, 18 August 2014
Week 5
Sensor construction bphR1/bphR2
'''bphR1 promoter:'''
Culturing bphR1 promoter (BBa_K1155001), received from Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam)
Incubation at 37°C overnight
Plate of 1 colony on LB-agar-Cam (25 ml Lb-Agar + 25µL Cam)
Incubation at 37°C overnight and glycerol stock
Sensor construction bphR1/bphR2
'''bphR1 promoter:'''Culturing bphR1 promoter (BBa_K1155001), received from Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam)
Incubation at 37°C overnight
Plate of 1 colony on LB-agar-Cam (25 ml Lb-Agar + 25µL Cam)
Incubation at 37°C overnight and glycerol stock
