|
|
| Line 56: |
Line 56: |
| | <!--main content --> | | <!--main content --> |
| | <table width="70%" align="center"> | | <table width="70%" align="center"> |
| | + | This year’s project is a continuation from the previous year, using the pGAPZ(alpha) vector system we are modifying the vector to iGEM standards to allow for successful purification and secretion of recombinant DNA. Thus far, pGAPZ(alpha) A and C have been modified and we have been actively working to modify pGAPZ(alpha) B. The glyceraldehydes-3-phosphate dehydrogenase expression vector has shown high levels of protein expression in methylotrophic yeast, Pichia Pastoris. The protein is enhanced by eukaryotic posttranslational modifications that the Pichia Pastoris is capable of through methanol inducible expression. Additionally, the pGAPZ(alpha) expression system allows for controlled secretion of a protein, making the purification of a protein relatively simple. |
| | + | <br /><br /> |
| | + | Using this method, the GSU iGEM team has been working to successfully insert and purify mambalgin, a protein component of the venom of Dendroaspis Polylepis, better known as Black Mamba. The mambalgin peptide is a powerful analgesic that directly blocks pain transmission in the peripheral nervous system (Diochot et al, 2012) by targeting acid sensing ion channels within nociceptors beneath the epidermis. Furthermore, recombinant purification of mambalgin could assist in developing anti-venom without the attendant risk of harvesting venom directly from snakes. |
| | | | |
| - |
| |
| - | <!--welcome box -->
| |
| - | <tr>
| |
| - | <td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
| |
| - | <h1 >WELCOME TO iGEM 2014! </h1>
| |
| - | <p>Your team has been approved and you are ready to start the iGEM season!
| |
| - | <br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
| |
| - | <br>
| |
| - | <p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Georgia_State&action=edit"style="color:#FFFFFF"> Click here to edit this page!</a> </p>
| |
| - | </td>
| |
| - | </tr>
| |
| - |
| |
| - | <tr> <td colspan="3" height="5px"> </td></tr>
| |
| - | <!-- end welcome box -->
| |
| - | <tr>
| |
| - |
| |
| - | <!--navigation menu -->
| |
| - | <td align="center" colspan="3">
| |
| - |
| |
| - | <table width="100%">
| |
| - | <tr heigth="15px"></tr>
| |
| - | <tr heigth="75px">
| |
| - |
| |
| - |
| |
| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
| |
| - | <a href="https://2014.igem.org/Team:Georgia_State"style="color:#000000">Home </a> </td>
| |
| - |
| |
| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
| |
| - | <a href="https://2014.igem.org/Team:Georgia_State/Team"style="color:#000000"> Team </a> </td>
| |
| - |
| |
| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
| |
| - | <a href="https://igem.org/Team.cgi?year=2014&team_name=Georgia_State"style="color:#000000"> Official Team Profile </a></td>
| |
| - |
| |
| - | <td style="border:1px solid black" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
| |
| - | <a href="https://2014.igem.org/Team:Georgia_State/Project"style="color:#000000"> Project</a></td>
| |
| - |
| |
| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
| |
| - | <a href="https://2014.igem.org/Team:Georgia_State/Parts"style="color:#000000"> Parts</a></td>
| |
| - |
| |
| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
| |
| - | <a href="https://2014.igem.org/Team:Georgia_State/Modeling"style="color:#000000"> Modeling</a></td>
| |
| - |
| |
| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
| |
| - | <a href="https://2014.igem.org/Team:Georgia_State/Notebook"style="color:#000000"> Notebook</a></td>
| |
| - |
| |
| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
| |
| - | <a href="https://2014.igem.org/Team:Georgia_State/Safety"style=" color:#000000"> Safety </a></td>
| |
| - |
| |
| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
| |
| - | <a href="https://2014.igem.org/Team:Georgia_State/Attributions"style="color:#000000"> Attributions </a></td>
| |
| - |
| |
| - |
| |
| - | <td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - |
| |
| - | <!--end navigation menu -->
| |
| - | </tr>
| |
| - |
| |
| - |
| |
| - | </tr>
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - |
| |
| - | <tr> <td colspan="3" height="15px"> </td></tr>
| |
| - | <tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
| |
| - | <tr> <td colspan="3" height="5px"> </td></tr>
| |
| - |
| |
| - |
| |
| - | <!--requirements section -->
| |
| - | <tr><td colspan="3"> <h3> Requirements </h3></td></tr>
| |
| - | <tr>
| |
| - | <td width="45%" valign="top">
| |
| - |
| |
| - | <p> Please be sure to keep these links, your audience will want to find your: </p>
| |
| - |
| |
| - | <!-- Links to other team pages -->
| |
| - | <ul>
| |
| - | <li><a href="https://2014.igem.org/Team:Georgia_State">Home</a> </li>
| |
| - | <li><a href="https://2014.igem.org/Team:Georgia_State/Team">Team</a> </li>
| |
| - | <li><a href="https://igem.org/Team.cgi?year=2013&team_name=Georgia_State">Official Team Profile</a> </li>
| |
| - | <li><a href="https://2014.igem.org/Team:Georgia_State/Project">Project</a> </li>
| |
| - | <li><a href="https://2014.igem.org/Team:Georgia_State/Parts">Parts</a> </li>
| |
| - | <li><a href="https://2014.igem.org/Team:Georgia_State/Modeling">Modeling</a> </li>
| |
| - | <li><a href="https://2014.igem.org/Team:Georgia_State/Notebook">Notebook</a> </li>
| |
| - | <li><a href="https://2014.igem.org/Team:Georgia_State/Safety">Safety</a> </li>
| |
| - | <li><a href="https://2014.igem.org/Team:Georgia_State/Attributions">Attributions</a> </li>
| |
| - |
| |
| - | </ul>
| |
| - |
| |
| - | </td>
| |
| - |
| |
| - | <td > </td>
| |
| - | <td width="45%">
| |
| - |
| |
| - | <p>There are a few wiki requirements teams must follow:</p>
| |
| - | <ul>
| |
| - | <li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload"> 2014.igem.org server</a>. </li>
| |
| - | <li>All pages must be created under the team’s name space.</li>
| |
| - | <li>As part of your documentation, keep the links from the menu to the left. </li>
| |
| - | <li>Do not use flash in wiki code. </li>
| |
| - | <li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
| |
| - | </ul>
| |
| - | <p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - |
| |
| - |
| |
| - | <tr> <td colspan="3" height="15px"> </td></tr>
| |
| - | <tr><td bgColor="#e7e7e7" colspan="3" height="1"> </tr>
| |
| - |
| |
| - |
| |
| - | <!--tips -->
| |
| - | <tr><td colspan="3" > <h3> Tips </h3></td></tr>
| |
| - |
| |
| - | <tr>
| |
| - | <td width="45%" valign="top">
| |
| - | <p>We are currently working on providing teams with some easy to use design templates.
| |
| - | <br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
| |
| - |
| |
| - | <ul>
| |
| - | <li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
| |
| - | <li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
| |
| - | <li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
| |
| - | <li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
| |
| - | <li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
| |
| - | </ul>
| |
| - |
| |
| - | <p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a> lists. </p>
| |
| - | </td>
| |
| - |
| |
| - | <td> </td>
| |
| - | <td width="45%">
| |
| - |
| |
| - | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
| |
| - |
| |
| - | <ul>
| |
| - | <li>We made the pgap vector standardized. </li>
| |
| - | <li>Be clear about what you are doing and what you plan to do.</li>
| |
| - | <li>You have a global audience! Consider the different backgrounds that your users come from.</li>
| |
| - | <li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
| |
| - | <li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
| |
| - | <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
| |
| - | <li>Have lots of fun! </li>
| |
| - | </ul>
| |
| - | </br>
| |
| - | </td>
| |
| - | </tr>
| |
| | </table> | | </table> |
"
