"
Team:HIT-Harbin/Judging
From 2014.igem.org
(Difference between revisions)
| Line 58: | Line 58: | ||
</div> | </div> | ||
</div> | </div> | ||
| + | </div> | ||
| + | |||
| + | <!--content --> | ||
| + | <div id="content"> | ||
| + | <div id="subheader-bar"> | ||
| + | <div class="site-description"><h4><span class="colored"><cufon class="cufon cufon-canvas" alt="Parts" style="width: 54px; height: 20px;"><canvas width="60" height="27" style="width: 60px; height: 27px; top: -7px; left: 1px;"></canvas><cufontext>Parts</cufontext></cufon></span></h4></div> | ||
| + | |||
| + | </div> | ||
| + | <p>Sequence optimization was done using JCat(Java Codon Adaptation Tool)[1] developed by Andreas Grote </p> | ||
| + | <h5 class="hashed"><span>CODON OPTIMIZATION FOR HRPS AND HRPR GENE</span></h5> | ||
| + | <div align="center"> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/f/f8/Hrps_sequence_cai_before_optimization.png"> | ||
| + | <p>Figure1: Codon Adaptation of hrpS gene in E.coli before optimization</p> | ||
| + | </div> | ||
| + | <p></p>CAI-Value of the sequence before optimization:0.2736. GC-Content of the sequence before optimization:59.17% | ||
| + | <div align="center"> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/d/d3/Hrps_sequence_cai_after_optimization.png"> | ||
| + | <p>Figure2: Codon Adaptation of hrpS gene in E.coli after optimization</p> | ||
| + | </div> | ||
| + | <p></p>CAI-Value of the optimized sequence:0.9832 GC-Content of the optimized sequence:53.60% | ||
| + | <div align="center"> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/a/a8/Hrpr_sequence_cai_before_optimization.png"> | ||
| + | <p>Figure3: Codon Adaptation of hrpR gene in E.coli before optimization</p> | ||
| + | </div> | ||
| + | <p></p>CAI-Value of the sequence before optimization:0.2490. GC-Content of the sequence before optimization:57.70 | ||
| + | <div align="center"> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/8/8b/Hrpr_sequence_cai_after_optimization.png"> | ||
| + | <p>Figure4: Codon Adaptation of hrpR gene in E.coli after optimization</p> | ||
| + | </div> | ||
| + | <p></p>CAI-Value of the optimized sequence:0.9721. GC-Content of the optimized sequence:53.69% | ||
| + | <h4>reference</h4> | ||
| + | <p></p>[1] Andreas Grote et al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Research 2005 Vol.33 | ||
</div> | </div> | ||
<div id="bottomNav"><a href="#top">Back To Top</a></div> | <div id="bottomNav"><a href="#top">Back To Top</a></div> | ||
| Line 111: | Line 143: | ||
</div> | </div> | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
</div> | </div> | ||
<script>toolbar('nav')</script> | <script>toolbar('nav')</script> | ||
Revision as of 12:53, 16 August 2014
Parts
Sequence optimization was done using JCat(Java Codon Adaptation Tool)[1] developed by Andreas Grote
CODON OPTIMIZATION FOR HRPS AND HRPR GENE
Figure1: Codon Adaptation of hrpS gene in E.coli before optimization
Figure2: Codon Adaptation of hrpS gene in E.coli after optimization
Figure3: Codon Adaptation of hrpR gene in E.coli before optimization
Figure4: Codon Adaptation of hrpR gene in E.coli after optimization
