Team:HIT-Harbin/Judging

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Revision as of 12:38, 16 August 2014

Project
  • Abstract
  • Background
  • Design
  • Modeling
  • Results
  • Future Work
Judging
  • Biobrick
  • Achievement
Wetlab
  • Protocols
  • Notebook
Team
  • Members
  • Advisors
  • Instructors
  • Attribution
  • Acknowledge
Human  practic
  • Country Profile
  • Qualification Trials
  • Wechat Platform
  • Video
  • Presentation

Parts

Sequence optimization was done using JCat(Java Codon Adaptation Tool)[1] developed by Andreas Grote

CODON OPTIMIZATION FOR HRPS AND HRPR GENE

Figure1: Codon Adaptation of hrpS gene in E.coli before optimization

CAI-Value of the sequence before optimization:0.2736. GC-Content of the sequence before optimization:59.17%

Figure2: Codon Adaptation of hrpS gene in E.coli after optimization

CAI-Value of the optimized sequence:0.9832 GC-Content of the optimized sequence:53.60%

Figure3: Codon Adaptation of hrpR gene in E.coli before optimization

CAI-Value of the sequence before optimization:0.2490. GC-Content of the sequence before optimization:57.70

Figure4: Codon Adaptation of hrpR gene in E.coli after optimization

CAI-Value of the optimized sequence:0.9721. GC-Content of the optimized sequence:53.69%

reference

[1] Andreas Grote et al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Research 2005 Vol.33

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