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| - | <style type="text/css">.conc { text-align: center } .title2 { text-align: center; font-family: calibri } .title4 { font-family: calibri }</style>
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| | <div class="week"> | | <div class="week"> |
| - | <h2 class=title2> <b> Antibiotic test </b></h2> | + | <h2 class=title2> Title</h2> |
| | <div class="CellCharacterization"> | | <div class="CellCharacterization"> |
| - | <h4 class=title4> <u> Preparation of antibiotic stocks </u> </h4>
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| | <p> | | <p> |
| - | <table>
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| - | <tr class = conc>
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| - | <td> <b>Antibiotic </b> </td>
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| - | <td> <b>Stock concentration </b> </td>
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| - | <td> <b>Protocol </b> </td>
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| - | </tr>
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| - | <tr>
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| - | <td>Kanamycin </td>
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| - | <td class= conc> 25 mg/mL </td>
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| - | <td> Weight 0.56g of Kanamycin powder, solubilization into 20mL of miliQ water. <br>Vortex 5min and filtration with a 200nm filter. </br> Stock : -20°C (aliquots of 1mL)</td>
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| - | </tr>
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| - | <tr>
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| - | <td> Streptomycin </td>
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| - | <td class= conc> 100 mg/mL </td>
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| - | <td> Weight 1g of Streptomycin powder, solubilization into 20mL of miliQ water.<br> Vortex 5min and filtration with a 200nm filter. </br> Stock : -20°C (aliquots of 1mL) </td>
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| - | </tr>
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| - | <tr>
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| - | <td> Amplicilin </td>
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| - | <td class= conc> 100 mg/mL </td>
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| - | <td> Weight 1g of Amplicilin powder, solubilization into 40mL of miliQ water. <br>Vortex 5min and filtration with a 200nm filter. </br> Stock : -20°C (aliquots of 1mL) </td>
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| - | </tr>
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| - | <tr>
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| - | <td> Tetracyclin</td>
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| - | <td class= conc> 15 mg/mL </td>
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| - | <td> Weight 0.6g of Tetracyclin powder, solubilization into 20mL of ethanol 50%. <br>Vortex 5min and filtration with a 200nm filter. </br> Stock : -20°C and hiden from light(aliquots of 1mL) </td>
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| - | </tr>
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| - | <tr>
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| - | <td> Chloramphenicol </td>
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| - | <td class= conc> 34 mg/mL </td>
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| - | <td> Weight 0.34g of Chloramphenicol powder, solubilization into 10mL of ethanol 100%. <br> Vortex 5min and filtration with a 200nm filter. </br> Stock : -20°C and hiden from light(aliquots of 1mL) </td>
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| - | </tr>
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| | | | |
| - | </table>
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