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Team:Paris Saclay/Notebook/August/12
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_clean-up Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_clean-up Protocol] | ||
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| + | ===Competents cells (CaCl2)=== | ||
| + | 'by Melanie' | ||
| + | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_competent_e.coli_by_CaCl2#Transformation protocol] | ||
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| + | stock of competents cells | ||
==Human Pratices== | ==Human Pratices== | ||
Revision as of 14:05, 12 August 2014
Contents |
Tuesday 11th August
Lab work
D - Lemon scent
PCR Clean-up of p cola and BBa_K517003
by Sean
PCR prepared on the 7th August.
Clean-up performed with the following protocol.
NB: in the present case we have 40 µl of each PCR result and we use 20 µl of elution buffer.
Competents cells (CaCl2)
'by Melanie' protocol
stock of competents cells
Human Pratices
Art & Design
by Terry
Resumption with the preculture made yesterday, expressing blue chromoprotein.
