Team:UT-Dallas/Notebook/8-04

From 2014.igem.org

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<h1 class="firstHeading" align="center"><-----------Day, Month Numerical Day, Year--------> </h1>
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<h1 class="firstHeading" align="center">Monday, August 4th, 2014</h1>
<center><p><a href="https://2014.igem.org/wiki/index.php?title=Team:UT-Dallas/Notebook/8-04&action=edit "style="color:#87A96B" >Click here to edit the page </a> </p></center>
<center><p><a href="https://2014.igem.org/wiki/index.php?title=Team:UT-Dallas/Notebook/8-04&action=edit "style="color:#87A96B" >Click here to edit the page </a> </p></center>
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   <p> Short coloquial description of what we did today and why. </p>
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   <p> We assembling the plasmid for the chromoproteins for bio-arts and potential outreach program later. We also assemble 7 of Kortne's reporter vectors that we didn't do before(Promoter+target-sequence+RBS+eYFP), 5 reporter vectors that we are sending for sequencing.</p>
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  <p> Tentative plan for tomorrow </P>
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       <th><font size="3">Today's tasks:</font>
       <th><font size="3">Today's tasks:</font>
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<p>Ligation. (over-night)</p>
 
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<p>Digest more promoters.</p>
 
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<p>Gel & gel purify reporter vectors for stuffs that Sam picked.</p>
 
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<LI> Digest more promoter vectors (we ran out).
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<LI> List item two
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<LI> Test digest gRNA and reporter vectors for ctxB.
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        <LI> Overnight ligation of chromoprotein with promoter, reporter vectors with promoter.
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Revision as of 18:32, 8 August 2014

Monday, August 4th, 2014

Click here to edit the page

We assembling the plasmid for the chromoproteins for bio-arts and potential outreach program later. We also assemble 7 of Kortne's reporter vectors that we didn't do before(Promoter+target-sequence+RBS+eYFP), 5 reporter vectors that we are sending for sequencing.
Today's tasks:
  • Digest more promoter vectors (we ran out).
  • Test digest gRNA and reporter vectors for ctxB.
  • Overnight ligation of chromoprotein with promoter, reporter vectors with promoter.

Retrieved from "http://2014.igem.org/Team:UT-Dallas/Notebook/8-04"