Team:Paris Saclay/Notebook/August/8

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(Created page with "=Friday 8th August= ==Lab work== ===D - Lemon Scent=== We have done PCR with pcola (geranyl synthase) and BBa517003: Green GotaqBuffer = 10µl MgCl2 = 4µl dNTP = 1µl Pri...")
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====stock of pcola plasmide====
====stock of pcola plasmide====
using the DNA kit extraction to extract pcola ()
using the DNA kit extraction to extract pcola ()
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==Human Pratices==
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===Art & Design===
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''by Terry''
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Sequel of [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7#Human_Pratices yesterday] molding attempt.
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The mold is cut delicately and the test piece removed.
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[[File:Photo agar test 003.jpg|950px]]
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Then, the mold is placed back in the becher and filled up with regular agar ( 20mg/ml )
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Result : FAILURE, the agar test piece has been ripped when unmolding. It was stuck to the mold.
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But the mold is still intact ! I cleaned it up and slightly covered the inward surface with liquid soap.
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The mold is filled up again and conserved in the refrigerator for 2 hours.
 +
 +
Result : SUCCESS, I slightly remoistened the gel with water for an easier unmolding. The agar test piece is perfectly removed.
 +
 +
[[File:Photo agar test 004.jpg|950px]]

Revision as of 15:32, 8 August 2014

Contents

Friday 8th August

Lab work

D - Lemon Scent

We have done PCR with pcola (geranyl synthase) and BBa517003:

Green GotaqBuffer = 10µl

MgCl2 = 4µl

dNTP = 1µl

Primer A = 1µM

Primer B = 1µM

DNA = 1µl

Gotaq DNA polymerase = 0.25µl


Oligo used : iPS81bis AND iPS82 (pcola) iPS69 and iPS68bis (BBa517003)

PCR cycle:

95° 2min

95° 30sec

49-55° 30 sec

72° 2min

71° 5min


stock of pcola plasmide

using the DNA kit extraction to extract pcola ()


Human Pratices

Art & Design

by Terry

Sequel of yesterday molding attempt.

The mold is cut delicately and the test piece removed.

Photo agar test 003.jpg

Then, the mold is placed back in the becher and filled up with regular agar ( 20mg/ml )

Result : FAILURE, the agar test piece has been ripped when unmolding. It was stuck to the mold.

But the mold is still intact ! I cleaned it up and slightly covered the inward surface with liquid soap. The mold is filled up again and conserved in the refrigerator for 2 hours.

Result : SUCCESS, I slightly remoistened the gel with water for an easier unmolding. The agar test piece is perfectly removed.

Photo agar test 004.jpg

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