Team:Paris Saclay/Notebook/July/31
From 2014.igem.org
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===C - Salicylate Inducible Suppressing System=== | ===C - Salicylate Inducible Suppressing System=== | ||
- | ====Transformation | + | ====Transformation of competent E.coli cells==== |
''by Fabio'' | ''by Fabio'' | ||
Revision as of 17:55, 6 August 2014
Contents |
Thursday 31st July
Lab work
C - Salicylate Inducible Suppressing System
Transformation of competent E.coli cells
by Fabio
We transformed in DH5α the Ligation's result made yesterday (BBa_J61051 and BBa_K228001).
Samples:
- Sample 2 μl
- Sample 1 μl
- DH5α Control
Results on 1st August morning:
- Success, grown very well (+- 100 colonies)
- Success, grown well (+- 50 colonies)
- Success, nothing has grown
D - Lemon scent
by Romain
2 Precultures of DY330 with pJBEI-6409 in 2ml LB and Cm.
Human Pratices
Reunion, Handled topics:
- Organisation of the Meeting with french teams
- Great discussion about Human Practices
- Discussion about the logo
Members present:
- Instructors and advisors: Alice and Solenne.
- Students: Arnaud, Fabio, Romain, Sean and Terry.