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Team:Paris Saclay/Notebook/July/9
From 2014.igem.org
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'''Romain & Sean''' | '''Romain & Sean''' | ||
| - | Plasmids used: DH5αZ1 | + | Plasmids used: DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A and B. |
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin®_Tissue Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin®_Tissue Protocol] | ||
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Plasmids used: | Plasmids used: | ||
| - | *DH5αZ1 | + | *DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A. |
| - | *DH5αZ1 | + | *DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, B. |
*XL1-Blue pCola-ges Kan | *XL1-Blue pCola-ges Kan | ||
*pJBEI-6409 Cm | *pJBEI-6409 Cm | ||
| Line 29: | Line 29: | ||
New liquid cultures of: | New liquid cultures of: | ||
*FNR-RBS-amilCp-Ter DH5αZ1 P5B1C3 Cm | *FNR-RBS-amilCp-Ter DH5αZ1 P5B1C3 Cm | ||
| - | *BBa B0015 dble ter | + | *BBa B0015 dble ter pSB1C3 Cm |
4x 5ml LB + 20μl Chloramphenicol + a little of bacteria. We incubate cultures at 37°C. | 4x 5ml LB + 20μl Chloramphenicol + a little of bacteria. We incubate cultures at 37°C. | ||
Revision as of 16:33, 6 August 2014
Contents |
Wednesday 9th July
Lab work
1 - Plasmid DNA Extraction:
Romain & Sean
Plasmids used: DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A and B.
2 - Electrophoresis:
Arnaud & Romain & Sean
Plasmids used:
- DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A.
- DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, B.
- XL1-Blue pCola-ges Kan
- pJBEI-6409 Cm
3 - New culture:
Romain
New liquid cultures of:
- FNR-RBS-amilCp-Ter DH5αZ1 P5B1C3 Cm
- BBa B0015 dble ter pSB1C3 Cm
4x 5ml LB + 20μl Chloramphenicol + a little of bacteria. We incubate cultures at 37°C.
Reunion
Conference with the team to discuss about the project. The handled topics were:
- Definition of Biobricks to make
- Planning of internships
- Definition of Biobricks to render
- Understanding the construction of consecutive genes
- Organisation and planning of experiences to do
- Sponsoring
- Wiki's progress
- Modelling's progress
Members present:
- Instructors and advisors: Alice, Olivier, Solenne and Sylvie.
- Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
