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Team:NTNU Trondheim/Protocols.html
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Revision as of 13:14, 6 August 2014
Team:NTNU Trondheim/Notebook
From 2014.igem.org
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Team:Cornell/notebook
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Media
- Liquid Broth (LB)
- Super Optimal Broth (S.O.C.)
- yB medium
- Synechocystis medium
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Techniques
- Gibson assembly
- DNA isolation and cleaning
- DNA digestion
- PCR
- NanoDrop
- 3A assembly
- Ligation
- Transformation (Escherichia coli)
- Transformation (Synechocystis sp. PCC 6803)
- Gel electrophoresis
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Plasmids
- Right flank
- Left flank
- Kanamycin
- Lac inducible promoter
- Glucose oxidase
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Organisms
- Escherichia coli DH5α
- Synechocystis sp. PCC 6803
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Calculations
- Transformation efficiency
- Enzyme amount
- DNA concentration
Notebook
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Week 23
(02/06 - 06/06)
June 3rd
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testtesttesttest test
Prepared solutions for future lab work.
June 4th
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{{{tech}}}
Made LB plates with ampicillin and ampicillin + kanamycin.
Week 19
(10/21 - 10/28)
