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Team:Paris Saclay/Notebook/August/4
From 2014.igem.org
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=Monday 4th August= | =Monday 4th August= | ||
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==Lab Work== | ==Lab Work== | ||
===A - The frame coli Odor free=== | ===A - The frame coli Odor free=== | ||
| - | |||
====Striate on Dishes==== | ====Striate on Dishes==== | ||
''by Juliette & Romain'' | ''by Juliette & Romain'' | ||
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We striated MG1655 and MG1655Z1 on LB and Kan dishes, from the striates prepared on LB dishes [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1 1st August] | We striated MG1655 and MG1655Z1 on LB and Kan dishes, from the striates prepared on LB dishes [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1 1st August] | ||
| - | === | + | ===C - Salicylate Inducible Suppressing System=== |
| + | ====Plasmid DNA Purification==== | ||
| + | ''by Eugene and Fabio'' | ||
| + | * Ligation of '''BBa_J61051''' and '''BBa_K228001''' made the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#Ligation 30th July] | ||
| + | From Liquid Culture made the [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#Liquid_Culture 1st August] | ||
| + | ====Digestion to check==== | ||
| + | ''In progress'' | ||
| + | |||
| + | ====Electrophoresis==== | ||
| + | ''In progress'' | ||
| + | |||
| + | ===D - Lemon scent=== | ||
====Gel electrophoresis of BBa_K762100==== | ====Gel electrophoresis of BBa_K762100==== | ||
''by Sean'' | ''by Sean'' | ||
Revision as of 10:40, 4 August 2014
Contents |
Monday 4th August
Lab Work
A - The frame coli Odor free
Striate on Dishes
by Juliette & Romain
We striated MG1655 and MG1655Z1 on LB and Kan dishes, from the striates prepared on LB dishes 1st August
C - Salicylate Inducible Suppressing System
Plasmid DNA Purification
by Eugene and Fabio
- Ligation of BBa_J61051 and BBa_K228001 made the 30th July
From Liquid Culture made the 1st August
Digestion to check
In progress
Electrophoresis
In progress
D - Lemon scent
Gel electrophoresis of BBa_K762100
by Sean
Samples used were prepared on the 30th July.
Results
From left to right: 5 µl of BBa_K762100 + "old" Phusion enzyme, 5 µl of BBa_K762100 + "new" Phusion enzyme
Electroporation
by Terry
Strain used: DY330 containing plasmid pJBEI6409.
We had to switch the Limonen Synthase cassette with the Apramycin resistance.
The culture has been placed at 42°C for 17 min then cooled 10 min before centrifugation.
Members there:
- Instructors and advisors: Alice, Solenne and Sylvie.
- Students: Eugene, Fabio, Hoang Vu, Juliette, Pierre, Romain, Sean and Terry.
