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Team:Paris Saclay/Notebook/August/4
From 2014.igem.org
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Terry.Mara (Talk | contribs) (→Electroporation) |
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| - | Strain used: DY330 | + | Strain used: DY330 containing plasmid pJBEI6409. |
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| + | We had to switch the Limonen Synthase cassette with the Apramycin resistance. | ||
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The culture has been placed at 42°C for 17 min then cooled 10 min before centrifugation. | The culture has been placed at 42°C for 17 min then cooled 10 min before centrifugation. | ||
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar] | [https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar] | ||
Revision as of 10:10, 4 August 2014
Contents |
Monday 4th August
Lab Work
A - The frame coli Odor free
Striate on Dishes
by Juliette & Romain
We striated MG1655 and MG1655Z1 on LB and Kan dishes, from the striates prepared on LB dishes 1st August
D - Lemon scent
Gel electrophoresis of BBa_K762100
by Sean
Samples used were prepared on the 30th July.
Results
From left to right: 5 µl of BBa_K762100 + "old" Phusion enzyme, 5 µl of BBa_K762100 + "new" Phusion enzyme
Electroporation
by Terry
Strain used: DY330 containing plasmid pJBEI6409.
We had to switch the Limonen Synthase cassette with the Apramycin resistance.
The culture has been placed at 42°C for 17 min then cooled 10 min before centrifugation.
